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The map label for this gene is ywoD

Identifier: 16080701

GI number: 16080701

Start: 3753933

End: 3755291

Strand: Reverse

Name: ywoD

Synonym: BSU36480

Alternate gene names: 16080701

Gene position: 3755291-3753933 (Counterclockwise)

Preceding gene: 255767785

Following gene: 16080700

Centisome position: 89.08

GC content: 47.46

Gene sequence:

>1359_bases
ATGTTATCGAAAAAAAGCCGAGCTGTTTTTTTAACAGCTGTGGCTTCGGGAACAATGCTGAATCCGCTGAACTCGTCTAT
GATCTCTCTTGCGCTCCATAGCATCCAGCATGAGTTTCACCTCTCGTTTACAACGGTTTCCTGGCTGATTTCCTCGTTTT
ACTTGGCGAGTGCCGTAGCGCAGCCCGTAACGGGAAAGCTCGGTGATTTGATTGGGCGGAAACGCTTGTTTTTATTCGGG
CTTATTCTTGTTGCGGTTTCTGCCATCGGGGCGCCGTTTGCGCCGACGTTTATGACATTATTGGTCATGCGGCTGTTTCA
ATCGGTGGGGAGCAGTGCGATTTATCCGTCAGGTGTCGGCTTGATTCGGAATCATATTCATGAGCGCCAAGCTTCAGCCT
TGGCTGTACTCTCTATTTTTGCGTCCGCCATGACGGCGCTGGGGCCGACAGCCGGCGGGTTTTTGATTGTATGGGGAGGC
TGGCCGGCGATCTTTATCGTTAATTTGCCGTTTATTATCCTCAGCTTCTTATTGGGCCTGTATATGTTCCCGAAAGATCA
GAAAAAAGGGGCAGGAATCAAAACCATTATTCGGCAGCTCGATATTCTTGGAATTGTTTTATTTGCCGGGGGAATCATTT
TCCTTCTATCCTTTTTGCTGTCGTTCAGCACGTCGCCGCATGCGGTTGAAGGTGTGCTTGGCTTGCTTCTGCTCTGTGCG
TTTGTCTGGCGCGAGCTGAAAACGGATAAGCCGTTTATTGATGTCCGTCTGTTTAAAACACAGCGAAACCTGTCAGCCGT
GTATGTTCAGTTTATTTTATTGAATGTTTTCTTCTATTGTTTATTTTTTGGTTTGCCAAGTTACTTTCAGGATGAAATGC
ATCTTTCTGTTCAAACGAGCGGCCTGTTTATGCTGTTTATGTCTGGAATGAGCATCGTTGTGTCACCTCTAACCGGCAAA
TGGATCGACCGATCTGGTGTCGTCAAGCCGATCTTTGCGGGAGCGCTTTTGATGACGGCTGGAGCCGTGCTCTTAACCAT
CTTTTTTATCAATGTCCAGACCATCGGTAAAGGCCTCATTCTGTCGCTCTTGGGTATAGGCTACGGACTGGGCAATGTCG
CGCTTCAGGCAGCGATGCTGGAAACGAGTCCTTCCAACATGGTAGGAACGACTTCAGGCCTGTTTCAAACCTGCCGTTAC
CTAGGATCGATTTTGTCTTCGGTGATCCTTGGCATTCTTTTTGGTAAAGAAATCACGGCAGCCCATTTTGATATGATGGG
AATCATTATGATTATAGCGGGCGGAGCAAGCCTGTTAATGGCTGTGAGATTTGCTGCATTAATGAAAACTGCATCTTAA

Upstream 100 bases:

>100_bases
CCACATTTAGCGATGAAGAGCATGAAGCCACACTGCGCTTTATTTTCCCGAGAATCGGTAAATCGCGGACAACGGAAGAG
TTTCTGGAACAAGTGAAATG

Downstream 100 bases:

>100_bases
GAAAGAGACGGGCTCTGAGGTCTGTCTCTTTTTCTTGTTTAAGCTGTTCAAATACAAACGGGAAAATTGTATATCATAGC
CAGTGTTATTGTCTGACTCT

Product: efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLSKKSRAVFLTAVASGTMLNPLNSSMISLALHSIQHEFHLSFTTVSWLISSFYLASAVAQPVTGKLGDLIGRKRLFLFG
LILVAVSAIGAPFAPTFMTLLVMRLFQSVGSSAIYPSGVGLIRNHIHERQASALAVLSIFASAMTALGPTAGGFLIVWGG
WPAIFIVNLPFIILSFLLGLYMFPKDQKKGAGIKTIIRQLDILGIVLFAGGIIFLLSFLLSFSTSPHAVEGVLGLLLLCA
FVWRELKTDKPFIDVRLFKTQRNLSAVYVQFILLNVFFYCLFFGLPSYFQDEMHLSVQTSGLFMLFMSGMSIVVSPLTGK
WIDRSGVVKPIFAGALLMTAGAVLLTIFFINVQTIGKGLILSLLGIGYGLGNVALQAAMLETSPSNMVGTTSGLFQTCRY
LGSILSSVILGILFGKEITAAHFDMMGIIMIIAGGASLLMAVRFAALMKTAS

Sequences:

>Translated_452_residues
MLSKKSRAVFLTAVASGTMLNPLNSSMISLALHSIQHEFHLSFTTVSWLISSFYLASAVAQPVTGKLGDLIGRKRLFLFG
LILVAVSAIGAPFAPTFMTLLVMRLFQSVGSSAIYPSGVGLIRNHIHERQASALAVLSIFASAMTALGPTAGGFLIVWGG
WPAIFIVNLPFIILSFLLGLYMFPKDQKKGAGIKTIIRQLDILGIVLFAGGIIFLLSFLLSFSTSPHAVEGVLGLLLLCA
FVWRELKTDKPFIDVRLFKTQRNLSAVYVQFILLNVFFYCLFFGLPSYFQDEMHLSVQTSGLFMLFMSGMSIVVSPLTGK
WIDRSGVVKPIFAGALLMTAGAVLLTIFFINVQTIGKGLILSLLGIGYGLGNVALQAAMLETSPSNMVGTTSGLFQTCRY
LGSILSSVILGILFGKEITAAHFDMMGIIMIIAGGASLLMAVRFAALMKTAS
>Mature_452_residues
MLSKKSRAVFLTAVASGTMLNPLNSSMISLALHSIQHEFHLSFTTVSWLISSFYLASAVAQPVTGKLGDLIGRKRLFLFG
LILVAVSAIGAPFAPTFMTLLVMRLFQSVGSSAIYPSGVGLIRNHIHERQASALAVLSIFASAMTALGPTAGGFLIVWGG
WPAIFIVNLPFIILSFLLGLYMFPKDQKKGAGIKTIIRQLDILGIVLFAGGIIFLLSFLLSFSTSPHAVEGVLGLLLLCA
FVWRELKTDKPFIDVRLFKTQRNLSAVYVQFILLNVFFYCLFFGLPSYFQDEMHLSVQTSGLFMLFMSGMSIVVSPLTGK
WIDRSGVVKPIFAGALLMTAGAVLLTIFFINVQTIGKGLILSLLGIGYGLGNVALQAAMLETSPSNMVGTTSGLFQTCRY
LGSILSSVILGILFGKEITAAHFDMMGIIMIIAGGASLLMAVRFAALMKTAS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1788710, Length=316, Percent_Identity=26.8987341772152, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081983, Length=392, Percent_Identity=22.4489795918367, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790195, Length=296, Percent_Identity=26.0135135135135, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789042, Length=319, Percent_Identity=25.705329153605, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWOD_BACSU (P94574)

Other databases:

- EMBL:   Z82987
- EMBL:   AL009126
- PIR:   G70064
- RefSeq:   NP_391529.1
- ProteinModelPortal:   P94574
- EnsemblBacteria:   EBBACT00000003451
- GeneID:   938533
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36480
- NMPDR:   fig|224308.1.peg.3655
- GenoList:   BSU36480
- GeneTree:   EBGT00050000000114
- HOGENOM:   HBG679341
- OMA:   FLIVWGG
- ProtClustDB:   CLSK888026
- BioCyc:   BSUB:BSU36480-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 48655; Mature: 48655

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12e6985c)-; HASH(0x1255840c)-; HASH(0x12802344)-; HASH(0x11e394ac)-; HASH(0x12e7c864)-; HASH(0x1290444c)-; HASH(0x124c9284)-; HASH(0x1245b934)-; HASH(0x12a6ad38)-; HASH(0x12e7c600)-; HASH(0x12e3b750)-; HASH(0x12791c54)-; HASH(0x12e2b07c)-; HASH(0x12a96940)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKKSRAVFLTAVASGTMLNPLNSSMISLALHSIQHEFHLSFTTVSWLISSFYLASAVA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPVTGKLGDLIGRKRLFLFGLILVAVSAIGAPFAPTFMTLLVMRLFQSVGSSAIYPSGVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
LIRNHIHERQASALAVLSIFASAMTALGPTAGGFLIVWGGWPAIFIVNLPFIILSFLLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
YMFPKDQKKGAGIKTIIRQLDILGIVLFAGGIIFLLSFLLSFSTSPHAVEGVLGLLLLCA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FVWRELKTDKPFIDVRLFKTQRNLSAVYVQFILLNVFFYCLFFGLPSYFQDEMHLSVQTS
HHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECC
GLFMLFMSGMSIVVSPLTGKWIDRSGVVKPIFAGALLMTAGAVLLTIFFINVQTIGKGLI
HHHHHHHCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLLGIGYGLGNVALQAAMLETSPSNMVGTTSGLFQTCRYLGSILSSVILGILFGKEITA
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AHFDMMGIIMIIAGGASLLMAVRFAALMKTAS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLSKKSRAVFLTAVASGTMLNPLNSSMISLALHSIQHEFHLSFTTVSWLISSFYLASAVA
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QPVTGKLGDLIGRKRLFLFGLILVAVSAIGAPFAPTFMTLLVMRLFQSVGSSAIYPSGVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
LIRNHIHERQASALAVLSIFASAMTALGPTAGGFLIVWGGWPAIFIVNLPFIILSFLLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
YMFPKDQKKGAGIKTIIRQLDILGIVLFAGGIIFLLSFLLSFSTSPHAVEGVLGLLLLCA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FVWRELKTDKPFIDVRLFKTQRNLSAVYVQFILLNVFFYCLFFGLPSYFQDEMHLSVQTS
HHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECC
GLFMLFMSGMSIVVSPLTGKWIDRSGVVKPIFAGALLMTAGAVLLTIFFINVQTIGKGLI
HHHHHHHCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLLGIGYGLGNVALQAAMLETSPSNMVGTTSGLFQTCRYLGSILSSVILGILFGKEITA
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AHFDMMGIIMIIAGGASLLMAVRFAALMKTAS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377