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The map label for this gene is mscL

Identifier: 16080689

GI number: 16080689

Start: 3743267

End: 3743659

Strand: Reverse

Name: mscL

Synonym: BSU36360

Alternate gene names: 16080689

Gene position: 3743659-3743267 (Counterclockwise)

Preceding gene: 255767783

Following gene: 16080687

Centisome position: 88.8

GC content: 46.06

Gene sequence:

>393_bases
ATGTGGAATGAATTTAAAGCCTTTGCCATGCGGGGGAATATCGTAGACCTTGCGATTGGTGTTGTGATAGGCGGCGCTTT
CGGAAAGATCGTCACATCTCTAGTAAATGATATCATTATGCCTTTGGTCGGTTTGCTGCTGGGAGGGCTTGACTTTTCAG
GCCTGTCGTTTACGTTTGGCGATGCTGTAGTGAAATATGGCAGCTTTATACAGACGATTGTGAACTTCTTAATTATATCG
TTCTCCATTTTTATCGTCATTCGGACTTTGAATGGGTTAAGGCGCAAAAAAGAAGCAGAAGAAGAGGCTGCGGAGGAAGC
GGTGGATGCGCAGGAGGAGCTGCTGAAAGAAATCAGAGACCTGCTGAAGCAGCAAGCAAAGTCGCCGGAATAA

Upstream 100 bases:

>100_bases
AGAACTGACTTTTGCCCTCGGAGAATAATTGAAAAAAGGCAGATGGCCAGCGGCACATTTGTCTTTTTTTCTTTACAAAT
ATAGAAAAGCAGGTGATTGT

Downstream 100 bases:

>100_bases
AAAAGATGCCGTTAGAAACGGCGTCTTTTTTTATCTCAATTTTCGTTTAGGTTCGTTCTGTGTCCTTCAGTTCTTTAGGG
CTTCCTGTTGTTTCAGCGAG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MWNEFKAFAMRGNIVDLAIGVVIGGAFGKIVTSLVNDIIMPLVGLLLGGLDFSGLSFTFGDAVVKYGSFIQTIVNFLIIS
FSIFIVIRTLNGLRRKKEAEEEAAEEAVDAQEELLKEIRDLLKQQAKSPE

Sequences:

>Translated_130_residues
MWNEFKAFAMRGNIVDLAIGVVIGGAFGKIVTSLVNDIIMPLVGLLLGGLDFSGLSFTFGDAVVKYGSFIQTIVNFLIIS
FSIFIVIRTLNGLRRKKEAEEEAAEEAVDAQEELLKEIRDLLKQQAKSPE
>Mature_130_residues
MWNEFKAFAMRGNIVDLAIGVVIGGAFGKIVTSLVNDIIMPLVGLLLGGLDFSGLSFTFGDAVVKYGSFIQTIVNFLIIS
FSIFIVIRTLNGLRRKKEAEEEAAEEAVDAQEELLKEIRDLLKQQAKSPE

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=129, Percent_Identity=57.3643410852713, Blast_Score=140, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BACSU (P94585)

Other databases:

- EMBL:   Z83337
- EMBL:   AL009126
- PIR:   E70065
- RefSeq:   NP_391517.1
- ProteinModelPortal:   P94585
- SMR:   P94585
- EnsemblBacteria:   EBBACT00000001553
- GeneID:   936909
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36360
- NMPDR:   fig|224308.1.peg.3643
- GenoList:   BSU36360
- GeneTree:   EBGT00050000002096
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   P94585
- ProtClustDB:   PRK13955
- BioCyc:   BSUB:BSU36360-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14280; Mature: 14280

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x129fdcbc)-; HASH(0xfec7378)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNEFKAFAMRGNIVDLAIGVVIGGAFGKIVTSLVNDIIMPLVGLLLGGLDFSGLSFTFG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DAVVKYGSFIQTIVNFLIISFSIFIVIRTLNGLRRKKEAEEEAAEEAVDAQEELLKEIRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKQQAKSPE
HHHHHHCCCH
>Mature Secondary Structure
MWNEFKAFAMRGNIVDLAIGVVIGGAFGKIVTSLVNDIIMPLVGLLLGGLDFSGLSFTFG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DAVVKYGSFIQTIVNFLIISFSIFIVIRTLNGLRRKKEAEEEAAEEAVDAQEELLKEIRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKQQAKSPE
HHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377