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The map label for this gene is rbsB

Identifier: 16080649

GI number: 16080649

Start: 3706145

End: 3707062

Strand: Direct

Name: rbsB

Synonym: BSU35960

Alternate gene names: 16080649

Gene position: 3706145-3707062 (Clockwise)

Preceding gene: 16080648

Following gene: 16080650

Centisome position: 87.91

GC content: 45.53

Gene sequence:

>918_bases
ATGAAAAAGGCTGTATCCGTCATTTTAACGTTATCATTATTTTTGTTAACCGCCTGTTCGCTTGAGCCTCCCCAATGGGC
AAAGCCATCAAACTCGGGGAACAAAAAGGAATTCACCATTGGCTTGTCCGTCTCAACGCTTAATAATCCTTTTTTTGTCT
CATTAAAAAAGGGTATCGAAAAAGAAGCTAAAAAACGGGGAATGAAAGTCATCATTGTTGATGCACAAAATGATTCATCG
AAACAGACGAGTGACGTGGAAGATTTAATTCAGCAAGGTGTTGATGCATTATTAATCAACCCGACTGATTCTTCGGCGAT
CTCAACGGCAGTAGAATCTGCAAACGCAGTCGGTGTGCCCGTCGTAACAATCGATCGATCTGCGGAACAAGGAAAAGTTG
AAACCCTCGTTGCTTCCGATAATGTAAAAGGCGGTGAAATGGCCGCGGCGTTTATTGCCGACAAACTTGGAAAAGGAGCA
AAGGTGGCAGAGCTTGAAGGCGTCCCCGGCGCATCTGCCACACGGGAACGCGGCTCAGGATTCCATAACATCGCAGACCA
AAAGCTCCAAGTTGTCACAAAACAATCAGCTGACTTTGACCGCACGAAAGGCCTGACTGTCATGGAAAACCTGCTGCAGG
GACATCCTGATATCCAAGCTGTTTTTGCTCACAACGATGAAATGGCGCTCGGTGCTCTCGAGGCAATTAACAGCTCGGGG
AAAGACATATTGGTGATCGGTTTTGACGGCAATAAAGATGCGCTCGCTTCCATTAAAGACAGAAAGCTGTCAGCCACCGT
CGCTCAGCAGCCTGAATTAATCGGTAAATTGGCTACAGAAGCAGCAGATGATATTTTGCACGGGAAAAAAGTGCAAAAAA
CAATATCCGCGCCTCTCAAACTAGAAACACAAAAATAA

Upstream 100 bases:

>100_bases
TGCTTGGCGTCTCATCATTTTATCAGCTGGTTGTCAAAGGGATTGTTATCTTAATTGCGGTATTGTTAGACCGCAAGAAG
TCAGCTTAGGAGGGTTTTAC

Downstream 100 bases:

>100_bases
TTGTCTGATGTTTAGGAACCTGCGATAACGTGAATAGAGATATAGAATGAATTATTTCTCTTACGTGAGGAGAGGAACGG
CATGAACAAACCAACAAAAC

Product: ribose ABC transporter ribose-binding lipoprotein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIEKEAKKRGMKVIIVDAQNDSS
KQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVPVVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGKGA
KVAELEGVPGASATRERGSGFHNIADQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSG
KDILVIGFDGNKDALASIKDRKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLKLETQK

Sequences:

>Translated_305_residues
MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIEKEAKKRGMKVIIVDAQNDSS
KQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVPVVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGKGA
KVAELEGVPGASATRERGSGFHNIADQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSG
KDILVIGFDGNKDALASIKDRKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLKLETQK
>Mature_305_residues
MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIEKEAKKRGMKVIIVDAQNDSS
KQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVPVVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGKGA
KVAELEGVPGASATRERGSGFHNIADQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSG
KDILVIGFDGNKDALASIKDRKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLKLETQK

Specific function: Involved in the high-affinity D-ribose membrane transport system

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family

Homologues:

Organism=Escherichia coli, GI1790192, Length=265, Percent_Identity=54.3396226415094, Blast_Score=286, Evalue=9e-79,
Organism=Escherichia coli, GI1790526, Length=246, Percent_Identity=36.1788617886179, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790674, Length=227, Percent_Identity=32.5991189427313, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790194, Length=270, Percent_Identity=28.5185185185185, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788473, Length=276, Percent_Identity=28.9855072463768, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788898, Length=321, Percent_Identity=25.5451713395639, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787948, Length=225, Percent_Identity=24.8888888888889, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788474, Length=253, Percent_Identity=28.0632411067194, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): RBSB_BACSU (P36949)

Other databases:

- EMBL:   Z92953
- EMBL:   AL009126
- EMBL:   Z25798
- PIR:   A69690
- RefSeq:   NP_391477.1
- ProteinModelPortal:   P36949
- SMR:   P36949
- EnsemblBacteria:   EBBACT00000000399
- GeneID:   936848
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35960
- NMPDR:   fig|224308.1.peg.3603
- GenoList:   BSU35960
- GeneTree:   EBGT00050000000779
- HOGENOM:   HBG617058
- OMA:   DAHDAIK
- PhylomeDB:   P36949
- ProtClustDB:   CLSK887992
- BioCyc:   BSUB:BSU35960-MONOMER
- InterPro:   IPR001761

Pfam domain/function: PF00532 Peripla_BP_1

EC number: NA

Molecular weight: Translated: 32227; Mature: 32227

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEHHHHHH
KEAKKRGMKVIIVDAQNDSSKQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVP
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCE
VVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGKGAKVAELEGVPGASATRERGSG
EEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCC
FHNIADQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSG
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCC
KDILVIGFDGNKDALASIKDRKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLK
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHCCCEE
LETQK
EECCC
>Mature Secondary Structure
MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEHHHHHH
KEAKKRGMKVIIVDAQNDSSKQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVP
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCE
VVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGKGAKVAELEGVPGASATRERGSG
EEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCC
FHNIADQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSG
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCC
KDILVIGFDGNKDALASIKDRKLSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLK
CEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHCCCEE
LETQK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236