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The map label for this gene is comFA

Identifier: 16080600

GI number: 16080600

Start: 3642167

End: 3643558

Strand: Reverse

Name: comFA

Synonym: BSU35470

Alternate gene names: 16080600

Gene position: 3643558-3642167 (Counterclockwise)

Preceding gene: 16080601

Following gene: 16080599

Centisome position: 86.43

GC content: 42.74

Gene sequence:

>1392_bases
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Upstream 100 bases:

>100_bases
AATCTCCGTTTTTAGAGCGGAGATTTTTTTATATTCTTATTTTAATAGTTGGACAGAAAATATTCATTCAGGCATACTGT
TTCGAAAGGAGGCGTGCTAT

Downstream 100 bases:

>100_bases
CCATTATTTGAAAACGTGGTACAGTATACTCATATAACCGATGATGAGGAGCTGTAAAAATGCTTGTCAATTCAAAAGAA
ATCGTAATGAAAGAACTGCT

Product: helicase competence protein

Products: NA

Alternate protein names: ATP-dependent helicase ComFA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Sequences:

>Translated_463_residues
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD
>Mature_463_residues
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Specific function: Involved in transformation (competence for DNA uptake). Required for DNA uptake but not for binding. May provide the driving force for transport of DNA through an aqueous channel

COG id: COG4098

COG function: function code L; Superfamily II DNA/RNA helicase required for DNA uptake (late competence protein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COMFA_BACSU (P39145)

Other databases:

- EMBL:   Z18629
- EMBL:   U56901
- EMBL:   AL009126
- PIR:   G69602
- RefSeq:   NP_391427.1
- ProteinModelPortal:   P39145
- EnsemblBacteria:   EBBACT00000002635
- GeneID:   938537
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35470
- NMPDR:   fig|224308.1.peg.3553
- GenoList:   BSU35470
- GeneTree:   EBGT00050000000888
- HOGENOM:   HBG535566
- OMA:   DEVDSFP
- ProtClustDB:   CLSK2393403
- BioCyc:   BSUB:BSU35470-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- SMART:   SM00487
- SMART:   SM00490

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII

EC number: =3.6.4.12

Molecular weight: Translated: 52566; Mature: 52566

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCEEECHHHHHH
NRCGQTDQRYFSFYHSSGKNKLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGK
HCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCC
LSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGIESALNQGLRVCIATPRTDVV
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHCCCCEEEEECCCCCEE
LELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
EHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCC
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFV
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCEE
WCGNWKKKLNRNKIPPAVKRWIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVH
EECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECC
AEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGVLGAESSIFTESALVQIAGRT
CHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCC
GRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCEEECHHHHHH
NRCGQTDQRYFSFYHSSGKNKLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGK
HCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCC
LSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGIESALNQGLRVCIATPRTDVV
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHCCCCEEEEECCCCCEE
LELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
EHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCC
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFV
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCEE
WCGNWKKKLNRNKIPPAVKRWIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVH
EECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECC
AEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGVLGAESSIFTESALVQIAGRT
CHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCC
GRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412657; 9384377