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The map label for this gene is hisZ

Identifier: 16080546

GI number: 16080546

Start: 3588185

End: 3589360

Strand: Reverse

Name: hisZ

Synonym: BSU34930

Alternate gene names: 16080546

Gene position: 3589360-3588185 (Counterclockwise)

Preceding gene: 16080549

Following gene: 16080545

Centisome position: 85.14

GC content: 49.23

Gene sequence:

>1176_bases
ATGTTTATGTTTGAAAAACCGCACGGCATGAGAGATACACTGCCCGGTTTATACGAAACGAAAAAAAAGGTGAGACGATC
GTTAACCGATTTGATTGATAAATGGGGATATCAATTTATGGAAACGCCGACACTGGAGTTTTACGATACCGTTGGCGTCC
AGTCAGCAATTGAAGAGCAGCAGCTGTTTAAGCTACTTGATCAGGACGGCAAGACATTGGTGCTTCGCCCGGATATGACG
GGGCCGATTGCAAGGGTGGCGGCATCGAAGCTTCTGAAACACGGTCATCCGCTAAGAGTCGGCTATGCGGCAAATGTATT
CAGGGCTCAGGAGCGTGAAGGCGGACGTCCGGCTGAGTTCGAGCAGGTCGGAGTGGAGTTAATCGGTGACGGCACGACGA
GCGCGGATGCGGAGGTCATTGCTTTAGTCGTCGGGGCATTAAAAAACGCTGGGCTGGCATCCTTTAAAATTGCAATTGGC
CATGCCGGCATTGCGGATGCTTTGTTTGTTGAGGTGCTCGGAAACGTTGAACGAGCTGATGTGCTGCGGAGGTTCTTATA
TGAAAAGAACTACGTCGGCTACAGAGAGCATGTCAAGTCTCTCCCGCTTTCCTCCATTGATAAAAGCAGGCTGCTTGAGC
TCCTTGAACTGCGGGGCGGTATAGAAGTATGCGGACGTGCCGAGGAAATCGTCGATTCTGCGCAAGGAAAAAGCGTGGTT
GATGAGCTGAAGGCGCTGTGGGACATTCTTGAGGATTACGGATGTACGGAAAATGTCCGCCTGGATCTGAATATGGTCAG
CCACATGAGCTATTACACAGGGATTTTATTTGAAGTGTACGCCGAGAATGTCGGTTTTGTCATTGGAAGCGGCGGCCGTT
ACAACAAGCTGCTGGGCCATTTTGATTCACCTGCACCGGCAACAGGCTTCGGGCTTCGGATCGACCGGCTGATTGAAGCC
CTTCATATGAAGGACGAACCTTGTGAAATAGACGCTGTTATTTTCAGCAAAGAGCAGCGGGCGCAAGCCATCGCTTATGC
GAATGAAGAACGCATGAAAGGGAACAAAGTGGTTCTTCAAGATTTATCGGGAATAGAAAATATCGACCAGATGACAAAAT
CTTTTGCAAACGTCACTTATTTTATCGGTGCCAGAAAGGAAGAGCAAAATGGGTAA

Upstream 100 bases:

>100_bases
GTTGATTTTCATTCTTTCATCTGCTAACATATTATCATATTAGCGAACTAAAGGATTCGGAATTTTCCTTCATAAGATAA
CAGCGGAGGTGCTCGGGTTG

Downstream 100 bases:

>100_bases
GTTACTCACAATGGCGATGCCAAAGGGCCGGATATTTGAAGAAGCGGCAGGGCTGCTGAGGCAGGCAGGCTACAGGCTCC
CTGAGGAGTTTGAAGATTCG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLFKLLDQDGKTLVLRPDMT
GPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIG
HAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVV
DELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFDSPAPATGFGLRIDRLIEA
LHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQDLSGIENIDQMTKSFANVTYFIGARKEEQNG

Sequences:

>Translated_391_residues
MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLFKLLDQDGKTLVLRPDMT
GPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIG
HAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVV
DELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFDSPAPATGFGLRIDRLIEA
LHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQDLSGIENIDQMTKSFANVTYFIGARKEEQNG
>Mature_391_residues
MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLFKLLDQDGKTLVLRPDMT
GPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIG
HAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVV
DELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFDSPAPATGFGLRIDRLIEA
LHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQDLSGIENIDQMTKSFANVTYFIGARKEEQNG

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=390, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI15029520, Length=344, Percent_Identity=25.8720930232558, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1788861, Length=364, Percent_Identity=25.2747252747253, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=360, Percent_Identity=25, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71993693, Length=362, Percent_Identity=24.8618784530387, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=366, Percent_Identity=22.1311475409836, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24643061, Length=375, Percent_Identity=22.9333333333333, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_BACSU (O34459)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   H69641
- RefSeq:   NP_391373.1
- ProteinModelPortal:   O34459
- SMR:   O34459
- EnsemblBacteria:   EBBACT00000002951
- GeneID:   936602
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34930
- NMPDR:   fig|224308.1.peg.3499
- GenoList:   BSU34930
- GeneTree:   EBGT00050000001169
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- ProtClustDB:   PRK12292
- BioCyc:   BSUB:BSU34930-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 43505; Mature: 43505

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQ
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
QLFKLLDQDGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEF
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCCHH
EQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERAD
HHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHH
VLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVV
HHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCHHHH
DELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGH
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHC
FDSPAPATGFGLRIDRLIEALHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQ
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHEEECCHHHHHHHHHCCHHHHCCCHHHHH
DLSGIENIDQMTKSFANVTYFIGARKEEQNG
HHHCHHHHHHHHHHHHCEEEEEECCCCCCCC
>Mature Secondary Structure
MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQ
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
QLFKLLDQDGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEF
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCCHH
EQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERAD
HHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHH
VLRRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVV
HHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCHHHH
DELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGH
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHC
FDSPAPATGFGLRIDRLIEALHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQ
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHEEECCHHHHHHHHHCCHHHHCCCHHHHH
DLSGIENIDQMTKSFANVTYFIGARKEEQNG
HHHCHHHHHHHHHHHHCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377