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The map label for this gene is hisA

Identifier: 16080541

GI number: 16080541

Start: 3584317

End: 3585054

Strand: Reverse

Name: hisA

Synonym: BSU34880

Alternate gene names: 16080541

Gene position: 3585054-3584317 (Counterclockwise)

Preceding gene: 16080542

Following gene: 16080540

Centisome position: 85.04

GC content: 48.24

Gene sequence:

>738_bases
ATGAGTGCTTTTACATTATATCCGGCAATTGATATGAGAAACGGCAAATGTGTTCGCCTTGTTCAAGGGGATTATAATAA
GGAAACGATATATGGCGATTCACCGTACGACATGGCTGAGTTATTTGAGAAAGAGGGTGCCGAATGGATCCATCTCGTTG
ATCTCGACGGGGCCAAAGAAGGGAAAAGAGTGAATGACCGCCATGTGATTGAGATTGCGCAAAAGCTGAACCTGAAAGTC
GAAATCGGCGGTGGGATCCGTTCTGAAAATGACGTCTATGAATATTTATCAGCAGGAGTCGAGCGGGTGATCCTGGGAAG
CTCAGCAGTTTCTAACCCTCCGTTTGTCAAAAAAATGCTGAAACAATATGGCGAGAAAATTGCAATCGGACTGGATGCGA
GAAACGGTTTTGTTTCAACAGAGGGATGGCTTGAAACATCAACCCTTAAGGCAACTGAACTGGGCAAAGAGCTAGCCAAT
GAAGGTGCGGAAGTCTTTATTTTTACTGACATCGCGACAGATGGAATGCTGTCAGGCCCCAATGTTAAAAGCACGGTCGA
GCTTGCTAAGGAAACGGGGAAATCGGTGATCGCTTCCGGCGGAGTCAGCTCCGTCGCGGATCTCGAAGCTCTCGCCCGCA
ATGAGGCGGACGGCGTCTCTGGAGCGATCATCGGAAAAGCGCTGTATACCAATCAGTTTACGCTGAGTGAGGCGCTTGAA
AGGGTGAAGCGCAAATGA

Upstream 100 bases:

>100_bases
ACGTCTTCGGCGCCCAATTTCATCCGGAAAAAAGCAGCACGGTCGGAATGTCTATTTTAACCCAATTTACGAAAATGGCA
GCAGAACAGAAGGTGAAAAA

Downstream 100 bases:

>100_bases
TCACAAAACGGATTATCCCATGCCTTGATGTCAAAGAAGGACGCGTTGTCAAAGGAATTCAATTTCTCGAATTGAAGGAT
GCCGGTGACCCAGTTGAACT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKEGKRVNDRHVIEIAQKLNLKV
EIGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKMLKQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELAN
EGAEVFIFTDIATDGMLSGPNVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALE
RVKRK

Sequences:

>Translated_245_residues
MSAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKEGKRVNDRHVIEIAQKLNLKV
EIGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKMLKQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELAN
EGAEVFIFTDIATDGMLSGPNVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALE
RVKRK
>Mature_244_residues
SAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKEGKRVNDRHVIEIAQKLNLKVE
IGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKMLKQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELANE
GAEVFIFTDIATDGMLSGPNVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALER
VKRK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=36.5145228215768, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=23.5042735042735, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BACSU (O35006)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   F69640
- RefSeq:   NP_391368.1
- ProteinModelPortal:   O35006
- SMR:   O35006
- EnsemblBacteria:   EBBACT00000003990
- GeneID:   936580
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34880
- NMPDR:   fig|224308.1.peg.3494
- GenoList:   BSU34880
- GeneTree:   EBGT00050000001074
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   O35006
- ProtClustDB:   PRK00748
- BioCyc:   BSUB:BSU34880-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26529; Mature: 26398

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00092 N6_MTASE

Important sites: ACT_SITE 11-11 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKE
CCCEEEECEEECCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GKRVNDRHVIEIAQKLNLKVEIGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKML
CCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCHHEEEECCCCCCCCHHHHHHH
KQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELANEGAEVFIFTDIATDGMLSGP
HHCCCEEEEEEECCCCEECCCCCEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALE
CHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHH
RVKRK
HHHCC
>Mature Secondary Structure 
SAFTLYPAIDMRNGKCVRLVQGDYNKETIYGDSPYDMAELFEKEGAEWIHLVDLDGAKE
CCEEEECEEECCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GKRVNDRHVIEIAQKLNLKVEIGGGIRSENDVYEYLSAGVERVILGSSAVSNPPFVKKML
CCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCHHEEEECCCCCCCCHHHHHHH
KQYGEKIAIGLDARNGFVSTEGWLETSTLKATELGKELANEGAEVFIFTDIATDGMLSGP
HHCCCEEEEEEECCCCEECCCCCEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NVKSTVELAKETGKSVIASGGVSSVADLEALARNEADGVSGAIIGKALYTNQFTLSEALE
CHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHH
RVKRK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377