Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 16080542

GI number: 16080542

Start: 3585051

End: 3585689

Strand: Reverse

Name: hisH

Synonym: BSU34890

Alternate gene names: 16080542

Gene position: 3585689-3585051 (Counterclockwise)

Preceding gene: 16080543

Following gene: 16080541

Centisome position: 85.06

GC content: 46.79

Gene sequence:

>639_bases
ATGATCGGCGTGATAGATTACGGAATGGGGAATTTATACAGTGTCTCAAAGGCGCTTGAACGTGTCGGCGTGCCGTATTT
TGTTTCTGAAAAGCCGGAGGAGCTGAAAGAAGCTGATGCTTTCATTTTGCCGGGAGTCGGTTCATTTGGAGACGCGATGG
ACAATTTGGGCTACACGAAGCTTGATCAATTGATTCACGATATGGTCTCAGAGGGAAGGCTGCTTCTCGGGATCTGCCTC
GGCATGCAGCTTTTATTTGAAGAAAGCGAAGAAAACGGCACCGCTTCAGGACTCGGGCTGTTAAAAGGAAAAGCAGTCAG
GCTGAAAGCAGAAGATGAAAAAGGAAACAAGCTGAAGGTTCCGCATATGGGCTGGAATCGTCTTTCTTTTCATAATGAAT
CTCCGCTGCTGACTAAAACAGAACAAGGCTACGCTTACTTCGTTCATTCCTATTACATTGACGGGATGGAGGAGAACGCG
CTTCTGGCAAGCGCCGATTATGGTGTACGAGTGCCTGCTGTAGTCGGAAAAAGAAACGTCTTCGGCGCCCAATTTCATCC
GGAAAAAAGCAGCACGGTCGGAATGTCTATTTTAACCCAATTTACGAAAATGGCAGCAGAACAGAAGGTGAAAAAATGA

Upstream 100 bases:

>100_bases
GATTGAAGCCGTTTTTAAAGCACTGGGACGTGCGCTTGATGAAGCGACAACGATCGATCCGCGAGTTAAAGGAATACCGT
CAACGAAAGGGATGCTGTAA

Downstream 100 bases:

>100_bases
GTGCTTTTACATTATATCCGGCAATTGATATGAGAAACGGCAAATGTGTTCGCCTTGTTCAAGGGGATTATAATAAGGAA
ACGATATATGGCGATTCACC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICL
GMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYIDGMEENA
LLASADYGVRVPAVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK

Sequences:

>Translated_212_residues
MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICL
GMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYIDGMEENA
LLASADYGVRVPAVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK
>Mature_212_residues
MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICL
GMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYIDGMEENA
LLASADYGVRVPAVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=203, Percent_Identity=38.423645320197, Blast_Score=117, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=30.8755760368664, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BACSU (O34565)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   D69641
- RefSeq:   NP_391369.1
- ProteinModelPortal:   O34565
- SMR:   O34565
- EnsemblBacteria:   EBBACT00000002611
- GeneID:   936590
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34890
- NMPDR:   fig|224308.1.peg.3495
- GenoList:   BSU34890
- GeneTree:   EBGT00050000000583
- HOGENOM:   HBG292341
- OMA:   EERFYFV
- ProtClustDB:   PRK13141
- BioCyc:   BSUB:BSU34890-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23303; Mature: 23303

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 79-79 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTK
CEEEEECCCCCHHHHHHHHHHCCCCEEECCCHHHHHHCCEEEECCCCCHHHHHHCCCHHH
LDQLIHDMVSEGRLLLGICLGMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKV
HHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCEEEC
PHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYIDGMEENALLASADYGVRVPAVVGKRNV
CCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCEEEEECCCCEECCCEECCCCC
FGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTK
CEEEEECCCCCHHHHHHHHHHCCCCEEECCCHHHHHHCCEEEECCCCCHHHHHHCCCHHH
LDQLIHDMVSEGRLLLGICLGMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKV
HHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCEEEC
PHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYIDGMEENALLASADYGVRVPAVVGKRNV
CCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCEEEEECCCCEECCCEECCCCC
FGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377