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The map label for this gene is hisI

Identifier: 16080539

GI number: 16080539

Start: 3582936

End: 3583565

Strand: Reverse

Name: hisI

Synonym: BSU34860

Alternate gene names: 16080539

Gene position: 3583565-3582936 (Counterclockwise)

Preceding gene: 16080540

Following gene: 16080538

Centisome position: 85.01

GC content: 50.32

Gene sequence:

>630_bases
ATGAAACAGGCAGATGAACTGCGTTTTAACGAGGACGGATTAATTCCTGCTATCGTGCAGGATGCGGCAAGCAAAGAAGT
GCTGACACTTGCGTATATGAACAAGGAATCGTACGAAAAAACGCTGGAAACGAAGGAAACATGGTTTTACAGCCGCTCAC
GCCAAGCGTTATGGCATAAAGGAGAAACCTCAGGGAACACGCAAGCCGTCAAAGGTATCCGGTATGACTGCGATCAAGAC
GCGCTGCTCGTACTCGTAGAACCGTCAGGCCCGGCATGCCACACTGGCAGCTACAGCTGTTTTACAAAGGAACAAACAGA
AGAACAGGCCGCGGACCGATTCGGCATCATGAACGAACTGGAGCGGGTGATCGCGGAACGCCAAGCGGAAATGCCTGAAG
GAGCTTACACCACGTACCTCTTCCGTGAAGGCGTCGATAAGATTTTGAAAAAAGTCGGAGAAGAAGCATCCGAAGTCATC
ATCGCCGCGAAAAACCGGGATCATGAGGAACTCAAATGGGAAGCGGCAGACCTGCTGTATCACCTGCTTGTCCTGCTCAG
AGAACAATCCCTGCCGCTGGATGATGTGCTTGACGTATTGAAAAAACGCCATTCTGAGATTGAAGAGTAA

Upstream 100 bases:

>100_bases
GAGAAGCCGACGCCGCGCTGGCCGCCTCAATTTTTCATTATAAAGAAACATCGATTAAAGAAGTGAAATCATACTTGAAA
GAGTACGGGGTGAATGTGAG

Downstream 100 bases:

>100_bases
AGAGCCGGATGATACGGCTCTTTTTGTTTTGTGAAAAAAGTAATAGGTATTTAGATTTATATTGGGTGTTATATATGATT
AGTTAATAAAAGGAGATCAA

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHKGETSGNTQAVKGIRYDCDQD
ALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNELERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVI
IAAKNRDHEELKWEAADLLYHLLVLLREQSLPLDDVLDVLKKRHSEIEE

Sequences:

>Translated_209_residues
MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHKGETSGNTQAVKGIRYDCDQD
ALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNELERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVI
IAAKNRDHEELKWEAADLLYHLLVLLREQSLPLDDVLDVLKKRHSEIEE
>Mature_209_residues
MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHKGETSGNTQAVKGIRYDCDQD
ALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNELERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVI
IAAKNRDHEELKWEAADLLYHLLVLLREQSLPLDDVLDVLKKRHSEIEE

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=193, Percent_Identity=49.740932642487, Blast_Score=188, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319819, Length=197, Percent_Identity=36.0406091370558, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_BACSU (O34912)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   E69641
- RefSeq:   NP_391366.1
- ProteinModelPortal:   O34912
- EnsemblBacteria:   EBBACT00000001247
- GeneID:   936552
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34860
- NMPDR:   fig|224308.1.peg.3492
- GenoList:   BSU34860
- GeneTree:   EBGT00050000000892
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- PhylomeDB:   O34912
- ProtClustDB:   PRK02759
- BioCyc:   BSUB:BSU34860-MONOMER
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 23901; Mature: 23901

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHK
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
GETSGNTQAVKGIRYDCDQDALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNEL
CCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVIIAAKNRDHEELKWEAADLLY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHH
HLLVLLREQSLPLDDVLDVLKKRHSEIEE
HHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHK
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCC
GETSGNTQAVKGIRYDCDQDALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNEL
CCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVIIAAKNRDHEELKWEAADLLY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHH
HLLVLLREQSLPLDDVLDVLKKRHSEIEE
HHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377