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The map label for this gene is cwlO

Identifier: 16080533

GI number: 16080533

Start: 3574363

End: 3575784

Strand: Reverse

Name: cwlO

Synonym: BSU34800

Alternate gene names: 16080533

Gene position: 3575784-3574363 (Counterclockwise)

Preceding gene: 16080534

Following gene: 16080532

Centisome position: 84.82

GC content: 40.79

Gene sequence:

>1422_bases
GTGAGAAAGAGTTTAATTACACTTGGTTTGGCTTCCGTCATCGGGACAAGCAGTTTTTTGATCCCATTTACAAGTAAAAC
TGCATCGGCGGAAACATTAGATGAAAAGAAACAAAAAATCGAAAGCAAGCAATCTGAGGTTGCTTCCAGCATTGAAGCGA
AGGAAAAAGAATTAACCGAGCTTCAGGAAAATCAATCAAAGATTGAAAAAGAACTGAAAGACATTAACGATAAGGCGCTT
GATACAAGCAACAAGATTGAAGATAAAAAAGAAGAAAACGATAAAACAAAAGAAGAAATCAAAAAACTGAAAAAAGAGAT
TAAAGAAACTGAAGCACGCATCGAAAAACGCAATGAAATCCTGAAAAAACGCGTTCGTTCTTTACAGGAAAGCGGCGGAT
CTCAAGGATACATAGATGTTCTTTTAGGATCAACAAGCTTTGGTGACTTTATCTCTCGTGCGACTGCGGTTTCATCTATT
GTGGACGCAGACAAAGATTTAATCAAACAGCAAGAGCAGGATAAAGCGAAGCTCGAAGATTCTGAAGCGGATTTGAATGA
CAAGCTGAAAGAAGTTCAAGCTGCCTTGGCTAAATTAGAAACAATGCAAAAAGACCTTGATAAACAGCTTAATGAAAAAG
ACAAGCTTTTCGACGAAGCAAAAGCAAGCCAAAAGAAAACGGCTAAAGCGATTTCTGAATTAAAATCAGAGGCGTCTGAA
CTTGCAAATCAAAAAGCAAATACTGAAGCTGAACAAGCACGCATCAAAAAAGAACAAGAAGCAGCGGCTGCTTTGATCAA
AAAGCAGGAAGAAGCACAAAAAGCATCTGATGAAACACAAACAGATGACAGTCAAACGGCGACAACTGAATCATCAAAAG
CAAGCTCATCTGATGATTCTTCAGACAATTCTTCAGACAATTCTTCTAACGGTTCATCAAACAGTTCGTCAAACGGCTCA
TCTTCTAAGAAGAGCAGCGGCTCAAACAGCAATTCAGGCGGCACTGTTATCAGCAACTCTGGCGGAATTGAAGGCGCGAT
CAGCGTTGGTTCAAGCATTGTAGGACAATCTCCGTACAAGTTTGGCGGCGGACGCACTCAGTCTGATATCAACAACCGTA
TTTTTGACTGCTCATCATTCGTACGCTGGGCATACGCTTCTGCAGGTGTTAACCTTGGACCTGTAGGCGGAACAACAACT
GATACGTTAGTTGGCAGAGGACAAGCTGTCAGCGCGTCTGAAATGAAACGCGGAGACCTTGTGTTCTTTGACACTTACAA
AACAAATGGACACGTAGGAATCTACTTAGGAAACGGTACTTTCCTAAACGACAATACATCTCATGGCGTATCTGTTGACT
CTATGAGCAATCCTTACTGGAAAGCAGCATTTAAAGGTGTTGTAAGACGTGTTGTTCAATAA

Upstream 100 bases:

>100_bases
AACAACCATCATTCCTCGTAGAGTATGAGGAACGAAACTTGATGTTCAAAATACGATTCCAAATGAGGACAGGTTCACAG
TAAAAGGGAGGATATAAAAA

Downstream 100 bases:

>100_bases
TAAATATGACAAGGGCCTTCTATAAACAGAAGGCTCTTTCTTTATTAACATATACCCTTTTCGAATACATTTAATCGTGT
TGAGCAAAAAAATAGCGTAT

Product: secreted cell wall DL-endopeptidase

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTELQENQSKIEKELKDINDKAL
DTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEILKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSI
VDADKDLIKQQEQDKAKLEDSEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASE
LANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDSSDNSSDNSSNGSSNSSSNGS
SSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYKFGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTT
DTLVGRGQAVSASEMKRGDLVFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ

Sequences:

>Translated_473_residues
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTELQENQSKIEKELKDINDKAL
DTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEILKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSI
VDADKDLIKQQEQDKAKLEDSEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASE
LANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDSSDNSSDNSSNGSSNSSSNGS
SSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYKFGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTT
DTLVGRGQAVSASEMKRGDLVFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ
>Mature_473_residues
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTELQENQSKIEKELKDINDKAL
DTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEILKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSI
VDADKDLIKQQEQDKAKLEDSEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASE
LANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDSSDNSSDNSSNGSSNSSSNGS
SSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYKFGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTT
DTLVGRGQAVSASEMKRGDLVFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ

Specific function: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity

COG id: COG3883

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted. Secreted, cell wall

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family

Homologues:

Organism=Escherichia coli, GI1787944, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788501, Length=107, Percent_Identity=38.3177570093458, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLO_BACSU (P40767)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- EMBL:   M22901
- PIR:   F70031
- RefSeq:   NP_391360.1
- ProteinModelPortal:   P40767
- SMR:   P40767
- EnsemblBacteria:   EBBACT00000000618
- GeneID:   936533
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34800
- NMPDR:   fig|224308.1.peg.3486
- GenoList:   BSU34800
- GeneTree:   EBGT00050000000149
- HOGENOM:   HBG421016
- OMA:   GYAMQFQ
- ProtClustDB:   CLSK887932
- BioCyc:   BSUB:BSU34800-MONOMER
- InterPro:   IPR000064

Pfam domain/function: PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 51034; Mature: 51034

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00801 TRANSKETOLASE_1

Important sites: ACT_SITE 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTE
CCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQENQSKIEKELKDINDKALDTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEI
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSIVDADKDLIKQQEQDKAKLED
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCC
SDNSSDNSSNGSSNSSSNGSSSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHCHHHHCCCCCC
FGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTTDTLVGRGQAVSASEMKRGDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCE
VFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ
EEEEEECCCCEEEEEECCCEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKSLITLGLASVIGTSSFLIPFTSKTASAETLDEKKQKIESKQSEVASSIEAKEKELTE
CCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQENQSKIEKELKDINDKALDTSNKIEDKKEENDKTKEEIKKLKKEIKETEARIEKRNEI
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKRVRSLQESGGSQGYIDVLLGSTSFGDFISRATAVSSIVDADKDLIKQQEQDKAKLED
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SEADLNDKLKEVQAALAKLETMQKDLDKQLNEKDKLFDEAKASQKKTAKAISELKSEASE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANQKANTEAEQARIKKEQEAAAALIKKQEEAQKASDETQTDDSQTATTESSKASSSDDS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCC
SDNSSDNSSNGSSNSSSNGSSSKKSSGSNSNSGGTVISNSGGIEGAISVGSSIVGQSPYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHCHHHHCCCCCC
FGGGRTQSDINNRIFDCSSFVRWAYASAGVNLGPVGGTTTDTLVGRGQAVSASEMKRGDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCE
VFFDTYKTNGHVGIYLGNGTFLNDNTSHGVSVDSMSNPYWKAAFKGVVRRVVQ
EEEEEECCCCEEEEEECCCEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3145906