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The map label for this gene is mdxD

Identifier: 16080515

GI number: 16080515

Start: 3555902

End: 3557671

Strand: Reverse

Name: mdxD

Synonym: BSU34620

Alternate gene names: 16080515

Gene position: 3557671-3555902 (Counterclockwise)

Preceding gene: 16080516

Following gene: 16080514

Centisome position: 84.39

GC content: 43.56

Gene sequence:

>1770_bases
ATGATGGAATATGCAGCGATTCATCACCAGCCATTCAGCACAGATGCTTATTCTTATGATGGGCGGACTGTGCATATCAA
GATTCGGACGAAAAAAGGTGATGCAGATCACATTCGATTCATTTGGGGTGATCCCTATGAGTACAACGACGGCAAATGGT
CGGCAAACGAGCAGCCGATGAGGAAAATTGCTGCTACAGAAATGCATGACTATTGGTTTGCTGAAGTGGTGCCGCCATTT
AGGCGTTTACAATATGCGTTTGTTGTAACAGACGATCATGAAGACATCTTTTTCGGAAGTTCGGGTGTATGCCCTTATAA
CGAAAAAACACTGGAAACGATTCATTATTACTTTAAATTTCCGTTTGTTCACGAGGCTGATACATTTCAAGCGCCTGAAT
GGGTCAAGTCAACGGTTTGGTATCAAATTTTTCCGGAGCGTTTTGCAAATGGAAGAGAAGACTTGTCACCGAAGAATGCA
TTGCCGTGGGGGAGCAAGGACCCGGACGTCAATGATTTTTTTGGAGGGGATTTACAAGGAATTGTTGACAAGCTTGATTA
TTTAGAGGATTTAGGGGTGAACGGCATTTATCTGACACCGATCTTTTCCGCGCCTTCTAATCATAAATACGACACGCTGG
ATTACTTTTCTATTGATCCGCATTTCGGGGATCCTGAGCTTTTTCGTACATTGGTCAGCCAGCTGCATCAGCGGGGGATG
AGAATTATGCTTGATGCGGTTTTTAACCATATAGGAAGCGCGTCTCCGCAATGGCAGGATGTCGTCAAAAACGGAGATCA
ATCCCGCTATAAGGACTGGTTCCATATTCACTCTTTTCCCGTCACAGACGACAATTATGACAGATTTGCTTTTACAGCTG
ATATGCCGAAGCTAAACACGGCTAATCCCGAGGTGCAGAAATATTTGCTTGATATTGCTTTGTACTGGATTCGCGAATTT
GACATTGACGGCTGGCGTTTGGATGTCGCAAATGAAGTGGATCACGTTTTTTGGAAGACATTTAGGCAGGCTGTATCTAC
TGAAAAGCCTGATGTTTATATTTTAGGTGAGATCTGGCATTCTGCCGAACCCTGGCTTAGAGGGGATGAATTTCATGCGG
CGATGAATTACCCTTTTACAGAGCCTATGATTGAATACTTTGCTGACCAGACGATCTCAGCTTCACGTATGGCTCACCGT
GTCAATGCACATTTGATGAATGGAATGAAGCAGGCCAATGAGGTGATGTTTAATTTACTGGATAGCCATGATACAAAGCG
CCTTTTGACCAGATGCCGCAATGATGAGAAGAAAGCACGCGCGTTGCTGGCCTTTATGTTTGCCCAGACAGGCTCACCAT
GCATTTATTACGGAACGGAAATCGGGCTGAACGGCGAAAATGATCCATTATGCCGGAAATGTATGGTTTGGGAAAAAGAG
AAGCAAAACCAAGACATGCTGCAATTTATGAAACGGTTAATTGCTTTACGCAAGCAGGAAAACACTTTATTGACTGAAGG
ACATCTTGAGTGGAATCTGCTGGACGACAAAAATGATTTCATTAGCTTTTCGCGGACTCTTGATGAAAAAATACTGATTT
ATTTCTTTAATCAAGGGAATGTGGTTCAACACGTCAGTTTACGAGAGTTGAATATTGATAGGAATAAGAAAATCTGTGAT
GCATGGACAGAGCAGCCGCTACAGCATCACGATGTCATTGCCGTTCAGCCGGGAGAATTTTTGATTTTGAGTGCTGCCGC
CCCTGTATAA

Upstream 100 bases:

>100_bases
GTTATCGGTAACAGGGTGAAAAGGATTGTTCCCACAATTTCATCATGATACTTTCAAACTATGAGCCGGCTTCAATAAAA
CGGAAATCAAAGGGGGAAAC

Downstream 100 bases:

>100_bases
CATTCTGTTATCGGTAACAAATCGAAAGTTGTTGTAACCGCTTTCTATAGGACAATACAATTGTTATAGTTTCGTAAATA
GAAGGAGGAAAAGAGATGGT

Product: glucan 1,4-alpha-maltohydrolase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMRKIAATEMHDYWFAEVVPPF
RRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFPFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNA
LPWGSKDPDVNDFFGGDLQGIVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGM
RIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNTANPEVQKYLLDIALYWIREF
DIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHR
VNAHLMNGMKQANEVMFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKE
KQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGNVVQHVSLRELNIDRNKKICD
AWTEQPLQHHDVIAVQPGEFLILSAAAPV

Sequences:

>Translated_589_residues
MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMRKIAATEMHDYWFAEVVPPF
RRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFPFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNA
LPWGSKDPDVNDFFGGDLQGIVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGM
RIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNTANPEVQKYLLDIALYWIREF
DIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHR
VNAHLMNGMKQANEVMFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKE
KQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGNVVQHVSLRELNIDRNKKICD
AWTEQPLQHHDVIAVQPGEFLILSAAAPV
>Mature_589_residues
MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMRKIAATEMHDYWFAEVVPPF
RRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFPFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNA
LPWGSKDPDVNDFFGGDLQGIVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGM
RIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNTANPEVQKYLLDIALYWIREF
DIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHR
VNAHLMNGMKQANEVMFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKE
KQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGNVVQHVSLRELNIDRNKKICD
AWTEQPLQHHDVIAVQPGEFLILSAAAPV

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=396, Percent_Identity=23.7373737373737, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786604, Length=467, Percent_Identity=34.0471092077088, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789995, Length=504, Percent_Identity=25.7936507936508, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790687, Length=388, Percent_Identity=26.2886597938144, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321726, Length=422, Percent_Identity=24.4075829383886, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322245, Length=410, Percent_Identity=22.9268292682927, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324416, Length=279, Percent_Identity=24.7311827956989, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321731, Length=405, Percent_Identity=21.7283950617284, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319776, Length=405, Percent_Identity=21.7283950617284, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=279, Percent_Identity=24.7311827956989, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=279, Percent_Identity=24.7311827956989, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24586599, Length=595, Percent_Identity=21.5126050420168, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24586587, Length=405, Percent_Identity=23.7037037037037, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24583745, Length=220, Percent_Identity=28.6363636363636, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583749, Length=233, Percent_Identity=27.4678111587983, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24583747, Length=233, Percent_Identity=27.8969957081545, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221330053, Length=231, Percent_Identity=28.1385281385281, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=28.4444444444444, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586589, Length=402, Percent_Identity=24.3781094527363, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45549022, Length=227, Percent_Identity=26.431718061674, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586597, Length=410, Percent_Identity=23.1707317073171, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24586593, Length=439, Percent_Identity=21.6400911161731, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 68854; Mature: 68854

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPM
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCEECCCCCCCCCHHHH
RKIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKF
HHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCEEECCCCCCCCCCHHHHHEEEHEEC
PFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQG
CEEECCCCCCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGM
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCH
RIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNT
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCC
ANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWH
CCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHC
SAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEVMFNLL
CCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCHHHHHHHHHHHH
KQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGN
CCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCC
VVQHVSLRELNIDRNKKICDAWTEQPLQHHDVIAVQPGEFLILSAAAPV
EEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCC
>Mature Secondary Structure
MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPM
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCEECCCCCCCCCHHHH
RKIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKF
HHHHHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCEEECCCCCCCCCCHHHHHEEEHEEC
PFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQG
CEEECCCCCCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGM
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCH
RIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNT
HHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCC
ANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWH
CCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHC
SAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEVMFNLL
CCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCHHHHHHHHHHHH
KQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGN
CCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCC
VVQHVSLRELNIDRNKKICDAWTEQPLQHHDVIAVQPGEFLILSAAAPV
EEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]