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The map label for this gene is mdxR

Identifier: 16080516

GI number: 16080516

Start: 3557784

End: 3558734

Strand: Reverse

Name: mdxR

Synonym: BSU34630

Alternate gene names: 16080516

Gene position: 3558734-3557784 (Counterclockwise)

Preceding gene: 16080517

Following gene: 16080515

Centisome position: 84.42

GC content: 44.9

Gene sequence:

>951_bases
ATGGCAACATTGTCAGATGTGGCGAAAAAAGCAAATGTTTCGAAAATGACGGTATCACGAGTGATCAATCACCCTGAGAC
GGTGACGGATGAATTGAAAAAGCTTGTTCATTCTGCGATGAAAGAGCTGAATTACATCCCTAATTATGCGGCGAGAGCGC
TCGTGCAAAACAGAACACAGGTTGTGAAACTATTGATACTTGAGGAAATGGATACGACAGAACCATATTACATGAACCTG
CTGACAGGAATCAGCAGGGAGCTGGATCGCAATCACTATGCTCTCCAGCTTGTGACAAGAAATTCTCTTAATATTGGCCA
ATGTGACGGCATTATTGCCACCGGCTTGAGAAAGAACGATTTTGAAGGTGTAATAAAAGCTTTTGAGAAGCCGCTCGTCG
TATTTGGACAAAATGAAATGGGCTATGACTTTATCGATGTGAATAACGAGAAAGGCACTTTTATGGCAACCCGCCATGTT
ATGGGGCTGGGGGAGCGGGAAGTTGTCTTTTTTGGCATTGATTTAGATGAGCCTTTCGAACGTGCAAGAGAACAGGGGTA
TATACGGGCGATGAATAAAAGCTTTAAAAAATCGAATATGTTTCGGATCGACAACAGTTCTAAGAAAAGTGAATGCCTTG
CAAGAGAACTGCTGAAATCCATGGACAATCAGGCCGCATTTGTCTGCGCATCTGATCGAATCGCGCTCGGCGTCATTCGT
GCGGCGCAATCCCTTGGAAAGCGAATTCCAGAAGACGTTGCGGTTACCGGGAATGACGGTGTGTTTCTTGATCGCATCTC
CTCGCCGCGCTTGACTACGGTCAGACAGCCTGTTGTTGAAATGGGGGAAGCCTGTGCGAAAATGTTGCTGAAAAAAATGA
ATGAAGACGGCGCTGCACAAGGAAGTCTATTTTTTGAACCAGAGTTAATTGTACGAGAATCAACGCTGTAA

Upstream 100 bases:

>100_bases
TCTCTTTTTTATTTCTCTTTGGCCTGCATCGTATATTTAGCAGTAATAGAGGACTTTATCACTAGTTTGGATAATAAGCA
CAGATAAGGAGGGGATGAAA

Downstream 100 bases:

>100_bases
GCGCCTTATTCTGTTATCGGTAACAGGGTGAAAAGGATTGTTCCCACAATTTCATCATGATACTTTCAAACTATGAGCCG
GCTTCAATAAAACGGAAATC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQVVKLLILEEMDTTEPYYMNL
LTGISRELDRNHYALQLVTRNSLNIGQCDGIIATGLRKNDFEGVIKAFEKPLVVFGQNEMGYDFIDVNNEKGTFMATRHV
MGLGEREVVFFGIDLDEPFERAREQGYIRAMNKSFKKSNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIR
AAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQGSLFFEPELIVRESTL

Sequences:

>Translated_316_residues
MATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQVVKLLILEEMDTTEPYYMNL
LTGISRELDRNHYALQLVTRNSLNIGQCDGIIATGLRKNDFEGVIKAFEKPLVVFGQNEMGYDFIDVNNEKGTFMATRHV
MGLGEREVVFFGIDLDEPFERAREQGYIRAMNKSFKKSNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIR
AAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQGSLFFEPELIVRESTL
>Mature_315_residues
ATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQVVKLLILEEMDTTEPYYMNLL
TGISRELDRNHYALQLVTRNSLNIGQCDGIIATGLRKNDFEGVIKAFEKPLVVFGQNEMGYDFIDVNNEKGTFMATRHVM
GLGEREVVFFGIDLDEPFERAREQGYIRAMNKSFKKSNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIRA
AQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQGSLFFEPELIVRESTL

Specific function: Repressor That Binds To The Purf Operator And Coregulates Other Genes For De Novo Purine Nucleotide Synthesis. It Is Involved In Regulation Of Purb, Purc, Purek, Purhd, Purl, Purmn And Guaba Expression. Binds Hypoxanthine And Guanine As Inducers. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=30.3834808259587, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=28.8690476190476, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=26.4264264264264, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=27.9279279279279, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789068, Length=299, Percent_Identity=27.0903010033445, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=26.1980830670927, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=27.1875, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=24.702380952381, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=27.3584905660377, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=24.1379310344828, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=23.8938053097345, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YVDE_BACSU (O06987)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   E70033
- RefSeq:   NP_391343.1
- ProteinModelPortal:   O06987
- SMR:   O06987
- EnsemblBacteria:   EBBACT00000002839
- GeneID:   936460
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34630
- NMPDR:   fig|224308.1.peg.3469
- GenoList:   BSU34630
- GeneTree:   EBGT00070000031749
- HOGENOM:   HBG399528
- OMA:   VEMGEAC
- ProtClustDB:   CLSK806715
- BioCyc:   BSUB:BSU34630-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 35440; Mature: 35309

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQ
CCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VVKLLILEEMDTTEPYYMNLLTGISRELDRNHYALQLVTRNSLNIGQCDGIIATGLRKND
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCH
FEGVIKAFEKPLVVFGQNEMGYDFIDVNNEKGTFMATRHVMGLGEREVVFFGIDLDEPFE
HHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEHHHHHCCCCCEEEEEECCCCCHHH
RAREQGYIRAMNKSFKKSNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIR
HHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
AAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQ
HHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSLFFEPELIVRESTL
CCEEECCCEEEECCCC
>Mature Secondary Structure 
ATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQ
CCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VVKLLILEEMDTTEPYYMNLLTGISRELDRNHYALQLVTRNSLNIGQCDGIIATGLRKND
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCH
FEGVIKAFEKPLVVFGQNEMGYDFIDVNNEKGTFMATRHVMGLGEREVVFFGIDLDEPFE
HHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEHHHHHCCCCCEEEEEECCCCCHHH
RAREQGYIRAMNKSFKKSNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIR
HHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
AAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQ
HHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSLFFEPELIVRESTL
CCEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377