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The map label for this gene is epsF

Identifier: 16080485

GI number: 16080485

Start: 3523270

End: 3524424

Strand: Reverse

Name: epsF

Synonym: BSU34320

Alternate gene names: 16080485

Gene position: 3524424-3523270 (Counterclockwise)

Preceding gene: 255767770

Following gene: 16080484

Centisome position: 83.6

GC content: 52.29

Gene sequence:

>1155_bases
ATGAATAGCAGCCAAAAGCGCGTGCTCCATGTTCTCAGCGGCATGAACAGGGGCGGCGCGGAAACCATGGTAATGAATTT
ATATCGGAAGATGGACAAAAGCAAAGTGCAATTTGATTTTTTAACGTATCGAAATGATCCGTGCGCTTATGATGAAGAGA
TTTTATCTTTAGGCGGGCGGCTTTTTTATGTCCCGAGCATTGGGCAAAGCAATCCCCTTACATTTGTGAGGAATGTGAGA
AACGCGATAAAAGAAAATGGGCCGTTCAGCGCCGTTCATGCGCACACGGATTTCCAAACGGGTTTTATCGCCCTTGCGGC
AAGGCTCGCCGGAGTGCCGGTCAGGGTATGCCACTCCCACAATACGTCTTGGAAGACCGGCTTCAACTGGAAGGATCGAT
TGCAGCTGCTCGTGTTCAGGCGGCTCATTTTGGCAAATGCGACAGCGCTGTGTGCCTGCGGAGAGGATGCGGGCAGGTTT
TTATTTGGACAGTCCAATATGGAGCGGGAGCGTGTTCACCTTCTTCCTAACGGGATTGACCTTGAGTTGTTCGCCCCAAA
TGGGCAGGCGGCTGATGAAGAAAAAGCAGCACGCGGCATTGCAGCCGACCGGCTCATCATTGGCCATGTGGCCCGGTTTC
ATGAAGTGAAAAACCACGCGTTCCTGTTGAAGCTTGCCGCACATCTCAAGGAAAGAGGCATTCGCTTTCAGCTCGTTCTG
GCGGGAGACGGGCCGTTGTGCGGGGAGATAGAGGAGGAGGCGCGGCAGCAGAATTTGCTATCAGACGTCCTCTTTTTAGG
CACGGAAGAACGGATCCATGAACTGATGCGAACATTCGATGTATTTGTCATGCCGTCTCTGTACGAAGGCTTGCCGGTTG
TGCTTGTGGAAGCGCAGGCGTCGGGGCTTCCATGCATCATTTCAGACAGCATTACAGAAAAAGTCGACGCCGGTCTCGGG
CTTGTCACAAGATTAAGTCTTTCTGAGCCGATCAGCGTCTGGGCTGAAACCATTGCAAGGGCGGCCGCCGCAGGCAGGCC
GAAGCGTGAGTTCATCAAAGAAACACTCGCTCAACTTGGCTACGATGCACAGCAAAATGTAGGAGCGCTGCTGAATGTAT
ACAACATCAGCACGGAAAAGGACCATAACCGATGA

Upstream 100 bases:

>100_bases
TATATCGCAAAACCGTTAATTCGCGCCTTTATGCCTGCAGCTGTGATGAATCGCTACCATAAAAAAAGAGTGATGAACCA
AAAGGAAGGGCTTGTCAAGC

Downstream 100 bases:

>100_bases
TTGTATATGCCGTCAATATGGGGATTGTATTTATTTGGTCTTGGTTCGCTAAAATGTGCGGCGGCCGTGATGATTCGCTT
GCCACGGGGTATCGGCCGAA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNSSQKRVLHVLSGMNRGGAETMVMNLYRKMDKSKVQFDFLTYRNDPCAYDEEILSLGGRLFYVPSIGQSNPLTFVRNVR
NAIKENGPFSAVHAHTDFQTGFIALAARLAGVPVRVCHSHNTSWKTGFNWKDRLQLLVFRRLILANATALCACGEDAGRF
LFGQSNMERERVHLLPNGIDLELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLAAHLKERGIRFQLVL
AGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPSLYEGLPVVLVEAQASGLPCIISDSITEKVDAGLG
LVTRLSLSEPISVWAETIARAAAAGRPKREFIKETLAQLGYDAQQNVGALLNVYNISTEKDHNR

Sequences:

>Translated_384_residues
MNSSQKRVLHVLSGMNRGGAETMVMNLYRKMDKSKVQFDFLTYRNDPCAYDEEILSLGGRLFYVPSIGQSNPLTFVRNVR
NAIKENGPFSAVHAHTDFQTGFIALAARLAGVPVRVCHSHNTSWKTGFNWKDRLQLLVFRRLILANATALCACGEDAGRF
LFGQSNMERERVHLLPNGIDLELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLAAHLKERGIRFQLVL
AGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPSLYEGLPVVLVEAQASGLPCIISDSITEKVDAGLG
LVTRLSLSEPISVWAETIARAAAAGRPKREFIKETLAQLGYDAQQNVGALLNVYNISTEKDHNR
>Mature_384_residues
MNSSQKRVLHVLSGMNRGGAETMVMNLYRKMDKSKVQFDFLTYRNDPCAYDEEILSLGGRLFYVPSIGQSNPLTFVRNVR
NAIKENGPFSAVHAHTDFQTGFIALAARLAGVPVRVCHSHNTSWKTGFNWKDRLQLLVFRRLILANATALCACGEDAGRF
LFGQSNMERERVHLLPNGIDLELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLAAHLKERGIRFQLVL
AGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPSLYEGLPVVLVEAQASGLPCIISDSITEKVDAGLG
LVTRLSLSEPISVWAETIARAAAAGRPKREFIKETLAQLGYDAQQNVGALLNVYNISTEKDHNR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSF_BACSU (P71055)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   E70036
- RefSeq:   NP_391312.1
- ProteinModelPortal:   P71055
- EnsemblBacteria:   EBBACT00000002251
- GeneID:   938631
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34320
- NMPDR:   fig|224308.1.peg.3438
- GenoList:   BSU34320
- GeneTree:   EBGT00070000032231
- HOGENOM:   HBG421733
- OMA:   RDITAVW
- ProtClustDB:   CLSK887912
- BioCyc:   BSUB:BSU34320-MONOMER
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42623; Mature: 42623

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSQKRVLHVLSGMNRGGAETMVMNLYRKMDKSKVQFDFLTYRNDPCAYDEEILSLGGR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHCCCE
LFYVPSIGQSNPLTFVRNVRNAIKENGPFSAVHAHTDFQTGFIALAARLAGVPVRVCHSH
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECC
NTSWKTGFNWKDRLQLLVFRRLILANATALCACGEDAGRFLFGQSNMERERVHLLPNGID
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCEEECCCCCCCHHHEECCCCCE
LELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLAAHLKERGIRFQLVL
EEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPSLYEGLPVVLVEAQA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SGLPCIISDSITEKVDAGLGLVTRLSLSEPISVWAETIARAAAAGRPKREFIKETLAQLG
CCCCEEECCHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
YDAQQNVGALLNVYNISTEKDHNR
CCCCCCHHHEEEEEECCCCCCCCC
>Mature Secondary Structure
MNSSQKRVLHVLSGMNRGGAETMVMNLYRKMDKSKVQFDFLTYRNDPCAYDEEILSLGGR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHCCCE
LFYVPSIGQSNPLTFVRNVRNAIKENGPFSAVHAHTDFQTGFIALAARLAGVPVRVCHSH
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECC
NTSWKTGFNWKDRLQLLVFRRLILANATALCACGEDAGRFLFGQSNMERERVHLLPNGID
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCEEECCCCCCCHHHEECCCCCE
LELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLAAHLKERGIRFQLVL
EEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPSLYEGLPVVLVEAQA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SGLPCIISDSITEKVDAGLGLVTRLSLSEPISVWAETIARAAAAGRPKREFIKETLAQLG
CCCCEEECCHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
YDAQQNVGALLNVYNISTEKDHNR
CCCCCCHHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377