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The map label for this gene is epsE

Identifier: 255767770

GI number: 255767770

Start: 3524417

End: 3525253

Strand: Reverse

Name: epsE

Synonym: BSU34330

Alternate gene names: 255767770

Gene position: 3525253-3524417 (Counterclockwise)

Preceding gene: 16080487

Following gene: 16080485

Centisome position: 83.62

GC content: 47.55

Gene sequence:

>837_bases
ATGAACTCAGGACCGAAAGTTTCTGTCATTATGGGCATTTATAATTGCGAACGCACTTTGGCAGAAAGCATAGAATCCAT
ACTCAGCCAATCCTATAAAAATTGGGAGCTGATTTTGTGCGATGATGCGTCAACAGACGGCACGCTCCGTATCGCGAAGC
AGTATGCCGCTCATTACAGCGACCGCATCAAACTGATTCAAAACAAAACAAATAAGCGGCTTGCCGCATCATTAAATCAT
TGTCTTTCGCATGCGACAGGCGATTATATCGCACGTCAGGACGGAGATGACCTTTCGTTTCCGCGCCGTCTGGAAAAGCA
GGTCGCGTTTTTAGAAAAGCACCGACACTATCAGGTGGTTGGCACCGGCATGCTTGTGTTTGATGAATTTGGCGTAAGAG
GCGCCCGCATTCTGCCTTCTGTTCCGGAGCCGGGCATCATGGCAAAAGGGACTCCATTTTGCCACGGCACGATTATGATG
AGAGCGAGTGCCTACCGCACGCTGAAAGGCTACCGGTCGGTGCGGCGGACGAGACGAATGGAAGATATTGATTTGTGGCT
TCGCTTTTTTGAAGAGGGCTTCAGGGGCTATAATCTTCAGGAAGCCTTGTATAAAGTGAGGGAAGACAGCGATGCATTCA
AACGGCGGTCATTTACGTATTCAATCGACAATGCCATTCTTGTCTATCAGGCGTGCAGACGCTTGAAGCTTCCTTTATCT
GATTACATATATATCGCAAAACCGTTAATTCGCGCCTTTATGCCTGCAGCTGTGATGAATCGCTACCATAAAAAAAGAGT
GATGAACCAAAAGGAAGGGCTTGTCAAGCATGAATAG

Upstream 100 bases:

>100_bases
GCCGAAAAACAGCCTTGCGCTTCTCGGAGGCGCGAACGGTGGAAGAAATGGCAGATATTTATTCCGCGTACATGGATATG
GATACAAAGGAGAAAAGCGT

Downstream 100 bases:

>100_bases
CAGCCAAAAGCGCGTGCTCCATGTTCTCAGCGGCATGAACAGGGGCGGCGCGGAAACCATGGTAATGAATTTATATCGGA
AGATGGACAAAAGCAAAGTG

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNSGPKVSVIMGIYNCERTLAESIESILSQSYKNWELILCDDASTDGTLRIAKQYAAHYSDRIKLIQNKTNKRLAASLNH
CLSHATGDYIARQDGDDLSFPRRLEKQVAFLEKHRHYQVVGTGMLVFDEFGVRGARILPSVPEPGIMAKGTPFCHGTIMM
RASAYRTLKGYRSVRRTRRMEDIDLWLRFFEEGFRGYNLQEALYKVREDSDAFKRRSFTYSIDNAILVYQACRRLKLPLS
DYIYIAKPLIRAFMPAAVMNRYHKKRVMNQKEGLVKHE

Sequences:

>Translated_278_residues
MNSGPKVSVIMGIYNCERTLAESIESILSQSYKNWELILCDDASTDGTLRIAKQYAAHYSDRIKLIQNKTNKRLAASLNH
CLSHATGDYIARQDGDDLSFPRRLEKQVAFLEKHRHYQVVGTGMLVFDEFGVRGARILPSVPEPGIMAKGTPFCHGTIMM
RASAYRTLKGYRSVRRTRRMEDIDLWLRFFEEGFRGYNLQEALYKVREDSDAFKRRSFTYSIDNAILVYQACRRLKLPLS
DYIYIAKPLIRAFMPAAVMNRYHKKRVMNQKEGLVKHE
>Mature_278_residues
MNSGPKVSVIMGIYNCERTLAESIESILSQSYKNWELILCDDASTDGTLRIAKQYAAHYSDRIKLIQNKTNKRLAASLNH
CLSHATGDYIARQDGDDLSFPRRLEKQVAFLEKHRHYQVVGTGMLVFDEFGVRGARILPSVPEPGIMAKGTPFCHGTIMM
RASAYRTLKGYRSVRRTRRMEDIDLWLRFFEEGFRGYNLQEALYKVREDSDAFKRRSFTYSIDNAILVYQACRRLKLPLS
DYIYIAKPLIRAFMPAAVMNRYHKKRVMNQKEGLVKHE

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Escherichia coli, GI1788372, Length=135, Percent_Identity=32.5925925925926, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790044, Length=105, Percent_Identity=35.2380952380952, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSE_BACSU (P71054)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   D70036
- RefSeq:   NP_391313.2
- HSSP:   P39621
- ProteinModelPortal:   P71054
- SMR:   P71054
- EnsemblBacteria:   EBBACT00000003183
- GeneID:   938633
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34330
- NMPDR:   fig|224308.1.peg.3439
- GenoList:   BSU34330
- GeneTree:   EBGT00050000000531
- HOGENOM:   HBG338337
- ProtClustDB:   CLSK887913
- BioCyc:   BSUB:BSU34330-MONOMER
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: NA

Molecular weight: Translated: 32148; Mature: 32148

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGPKVSVIMGIYNCERTLAESIESILSQSYKNWELILCDDASTDGTLRIAKQYAAHYS
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
DRIKLIQNKTNKRLAASLNHCLSHATGDYIARQDGDDLSFPRRLEKQVAFLEKHRHYQVV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
GTGMLVFDEFGVRGARILPSVPEPGIMAKGTPFCHGTIMMRASAYRTLKGYRSVRRTRRM
ECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EDIDLWLRFFEEGFRGYNLQEALYKVREDSDAFKRRSFTYSIDNAILVYQACRRLKLPLS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCEEECCCHHHHHHHHHHCCCCHH
DYIYIAKPLIRAFMPAAVMNRYHKKRVMNQKEGLVKHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNSGPKVSVIMGIYNCERTLAESIESILSQSYKNWELILCDDASTDGTLRIAKQYAAHYS
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
DRIKLIQNKTNKRLAASLNHCLSHATGDYIARQDGDDLSFPRRLEKQVAFLEKHRHYQVV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
GTGMLVFDEFGVRGARILPSVPEPGIMAKGTPFCHGTIMMRASAYRTLKGYRSVRRTRRM
ECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EDIDLWLRFFEEGFRGYNLQEALYKVREDSDAFKRRSFTYSIDNAILVYQACRRLKLPLS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCEEECCCHHHHHHHHHHCCCCHH
DYIYIAKPLIRAFMPAAVMNRYHKKRVMNQKEGLVKHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377