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The map label for this gene is epsI

Identifier: 16080482

GI number: 16080482

Start: 3520030

End: 3521106

Strand: Reverse

Name: epsI

Synonym: BSU34290

Alternate gene names: 16080482

Gene position: 3521106-3520030 (Counterclockwise)

Preceding gene: 16080483

Following gene: 16080481

Centisome position: 83.53

GC content: 49.12

Gene sequence:

>1077_bases
ATGTCGTTACAATCGTTGAAAATCAATTTTGCAGAATGGCTGCTGCTAAAGGTCAAATACCCGTCCCAATATTGGCTGGG
AGCGGCAGATCAACCGGTAAAGGCCGCAGCACATCAGAAAAAAATCATACTGACCCTGCTGCCGTCCCATGACAATTTGG
GAGATCACGCAATTGCTTATGCCAGCAAGGCATTTCTTGAGCAAGAATACCCGGACTTTGACATCGTCGAGGTCGATATG
AAGGACATTTACAAATCAGCAAAAAGCCTGATCCGCTCGCGCCATCCGGAGGATATGGTCTTTATCATCGGCGGCGGAAA
CATGGGGGATTTATACCGTTATGAGGAGTGGACGCGCCGCTTCATCATTAAAACATTCCATGACTATCGGGTTGTCCAGC
TTCCGGCAACGGCTCATTTTTCTGACACGAAAAAAGGGCGCAAAGAGCTGAAACGGGCACAGAAAATTTATAATGCGCAC
CCCGGCCTATTGCTGATGGCGCGGGATGAAACAACGTATCAATTTATGAAACAGCATTTTCAAGAAAAAACAATTTTGAA
GCAGCCGGACATGGTGCTGTATTTAGACAGAAGCAAGGCTCCCGCAGAACGCGAAGGGGTTTATATGTGTTTGCGCGAGG
ATCAGGAAAGCGTGCTTCAGGAGGAGCAGAGGAACCGGGTCAAGGCTGCGCTATGTGAGGAATTCGGCGAGATCAAATCC
TTTACGACAACGATCGGCCGCCGGGTCAGCCGCGATACACGCGAACATGAACTTGAAGCACTGTGGTCTAAGCTGCAAAG
CGCAGAAGCCGTCGTCACTGACAGGCTTCATGGCATGATTTTTTGCGCGCTGACAGGAACGCCGTGTGTTGTCATTCGCT
CCTTTGACCATAAGGTGATGGAGGGCTATCAATGGCTTAAAGACATCCCGTTCATGAAGCTGATAGAACATCCGGAGCCA
GAGCGCGTAACAGCCGCAGTCAATGAGCTTTTAACAAAAGAAACATCCCGTGCAGGCTTTCCGAGAGATGTGTATTTTAA
AGGTCTGCGTGACAAAATCAGCGGTGAAGCGCAATGA

Upstream 100 bases:

>100_bases
CTTCTGATGGCGAAATACAGAATGATTCCGTTCGTCGCTATGGCATCGGCTGTGTATCAGCGGGTGATCGAGTACAAAAT
GAGAAACAGAGGGTGAGGAC

Downstream 100 bases:

>100_bases
TCCCGCTCGTCAGCATTATTGTCCCGATGTATAATGTTGAACCATTTATAGAAGAGTGCATTGATTCTTTGCTTCGTCAA
ACGCTTTCTGATATTGAAAT

Product: polysaccharide pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSLQSLKINFAEWLLLKVKYPSQYWLGAADQPVKAAAHQKKIILTLLPSHDNLGDHAIAYASKAFLEQEYPDFDIVEVDM
KDIYKSAKSLIRSRHPEDMVFIIGGGNMGDLYRYEEWTRRFIIKTFHDYRVVQLPATAHFSDTKKGRKELKRAQKIYNAH
PGLLLMARDETTYQFMKQHFQEKTILKQPDMVLYLDRSKAPAEREGVYMCLREDQESVLQEEQRNRVKAALCEEFGEIKS
FTTTIGRRVSRDTREHELEALWSKLQSAEAVVTDRLHGMIFCALTGTPCVVIRSFDHKVMEGYQWLKDIPFMKLIEHPEP
ERVTAAVNELLTKETSRAGFPRDVYFKGLRDKISGEAQ

Sequences:

>Translated_358_residues
MSLQSLKINFAEWLLLKVKYPSQYWLGAADQPVKAAAHQKKIILTLLPSHDNLGDHAIAYASKAFLEQEYPDFDIVEVDM
KDIYKSAKSLIRSRHPEDMVFIIGGGNMGDLYRYEEWTRRFIIKTFHDYRVVQLPATAHFSDTKKGRKELKRAQKIYNAH
PGLLLMARDETTYQFMKQHFQEKTILKQPDMVLYLDRSKAPAEREGVYMCLREDQESVLQEEQRNRVKAALCEEFGEIKS
FTTTIGRRVSRDTREHELEALWSKLQSAEAVVTDRLHGMIFCALTGTPCVVIRSFDHKVMEGYQWLKDIPFMKLIEHPEP
ERVTAAVNELLTKETSRAGFPRDVYFKGLRDKISGEAQ
>Mature_357_residues
SLQSLKINFAEWLLLKVKYPSQYWLGAADQPVKAAAHQKKIILTLLPSHDNLGDHAIAYASKAFLEQEYPDFDIVEVDMK
DIYKSAKSLIRSRHPEDMVFIIGGGNMGDLYRYEEWTRRFIIKTFHDYRVVQLPATAHFSDTKKGRKELKRAQKIYNAHP
GLLLMARDETTYQFMKQHFQEKTILKQPDMVLYLDRSKAPAEREGVYMCLREDQESVLQEEQRNRVKAALCEEFGEIKSF
TTTIGRRVSRDTREHELEALWSKLQSAEAVVTDRLHGMIFCALTGTPCVVIRSFDHKVMEGYQWLKDIPFMKLIEHPEPE
RVTAAVNELLTKETSRAGFPRDVYFKGLRDKISGEAQ

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles

COG id: COG5039

COG function: function code GM; Exopolysaccharide biosynthesis protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSI_BACSU (P71058)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   H70036
- RefSeq:   NP_391309.1
- ProteinModelPortal:   P71058
- EnsemblBacteria:   EBBACT00000003989
- GeneID:   937256
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34290
- NMPDR:   fig|224308.1.peg.3435
- GenoList:   BSU34290
- GeneTree:   EBGT00050000002465
- HOGENOM:   HBG338334
- OMA:   HGMIFCA
- ProtClustDB:   CLSK887909
- BioCyc:   BSUB:BSU34290-MONOMER
- InterPro:   IPR007345

Pfam domain/function: PF04230 PS_pyruv_trans

EC number: NA

Molecular weight: Translated: 41509; Mature: 41378

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQSLKINFAEWLLLKVKYPSQYWLGAADQPVKAAAHQKKIILTLLPSHDNLGDHAIAY
CCCCHHEECHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHH
ASKAFLEQEYPDFDIVEVDMKDIYKSAKSLIRSRHPEDMVFIIGGGNMGDLYRYEEWTRR
HHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
FIIKTFHDYRVVQLPATAHFSDTKKGRKELKRAQKIYNAHPGLLLMARDETTYQFMKQHF
HHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
QEKTILKQPDMVLYLDRSKAPAEREGVYMCLREDQESVLQEEQRNRVKAALCEEFGEIKS
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FTTTIGRRVSRDTREHELEALWSKLQSAEAVVTDRLHGMIFCALTGTPCVVIRSFDHKVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHH
EGYQWLKDIPFMKLIEHPEPERVTAAVNELLTKETSRAGFPRDVYFKGLRDKISGEAQ
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLQSLKINFAEWLLLKVKYPSQYWLGAADQPVKAAAHQKKIILTLLPSHDNLGDHAIAY
CCCHHEECHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHH
ASKAFLEQEYPDFDIVEVDMKDIYKSAKSLIRSRHPEDMVFIIGGGNMGDLYRYEEWTRR
HHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
FIIKTFHDYRVVQLPATAHFSDTKKGRKELKRAQKIYNAHPGLLLMARDETTYQFMKQHF
HHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
QEKTILKQPDMVLYLDRSKAPAEREGVYMCLREDQESVLQEEQRNRVKAALCEEFGEIKS
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FTTTIGRRVSRDTREHELEALWSKLQSAEAVVTDRLHGMIFCALTGTPCVVIRSFDHKVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHH
EGYQWLKDIPFMKLIEHPEPERVTAAVNELLTKETSRAGFPRDVYFKGLRDKISGEAQ
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377