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The map label for this gene is epsJ

Identifier: 16080481

GI number: 16080481

Start: 3518999

End: 3520033

Strand: Reverse

Name: epsJ

Synonym: BSU34280

Alternate gene names: 16080481

Gene position: 3520033-3518999 (Counterclockwise)

Preceding gene: 16080482

Following gene: 255767769

Centisome position: 83.5

GC content: 46.57

Gene sequence:

>1035_bases
ATGATCCCGCTCGTCAGCATTATTGTCCCGATGTATAATGTTGAACCATTTATAGAAGAGTGCATTGATTCTTTGCTTCG
TCAAACGCTTTCTGATATTGAAATCATCCTCGTGAATGACGGAACACCGGATCGTTCAGGCGAAATTGCAGAGGACTATG
CAAAACGGGATGCGAGAATCCGGGTCATTCATCAGGCAAACGGCGGGCTTAGTTCAGCGCGAAATACGGGAATAAAGGCC
GCGCGGGGCACTTACATCGGCTTTGTAGACGGAGACGATTATGTATCATCCGCCATGTTCCAGAGGCTGACTGAAGAAGC
GGAGCAAAATCAGCTTGACATCGTCGGATGCGGTTTTTACAAGCAGTCATCGGACAGGCGGACATATGTGCCGCCGCAGC
TTGAGGCAAACCGCGTGCTGACGAAACCAGAAATGACTGAACAGCTTAAACATGCTCACGAAACGAGATTTATCTGGTAT
GTATGGCGTTATCTTTACCGTCGTGAGCTTTTTGAAAGGGCGAATCTGCTGTTTGATGAAGACATCCGTTTTGCTGAAGA
CTCTCCCTTTAATTTGTCCGCTTTTCGCGAAGCGGAGCGGGTGAAAATGCTTGATGAAGGATTGTACATTTATCGTGAAA
ACCCGAACAGCCTGACAGAAATCCCTTATAAGCCGGCGATGGATGAACATCTTCAAAAACAATATCAGGCTAAAATCGCA
TTCTACAATCACTACGGCTTAGCAGGCGCATGTAAAGAAGATTTGAATGTGTACATTTGCAGGCACCAGCTTCCGATGCT
TTTGGCAAATGCCTGTGCTTCTCCGAATTCGCCGAAAGACATCAAAAAGAAGATCAGACAGATTTTATCCTATGACATGG
TGCGGCAGGCTGTCAGACATACACCGTTTCAGCATGAGAAATTATTAAGAGGAGAGCGTTTGGTATTAGCACTGTGTAAA
TGGCGGCTCACTTTTCTCATCAAGCTGTTTTTCGAGCAGCGGGGGACAATGAAAGGCAGTGCGAAGCAGGCATGA

Upstream 100 bases:

>100_bases
CCGCAGTCAATGAGCTTTTAACAAAAGAAACATCCCGTGCAGGCTTTCCGAGAGATGTGTATTTTAAAGGTCTGCGTGAC
AAAATCAGCGGTGAAGCGCA

Downstream 100 bases:

>100_bases
AATTCACGATAAATTTCAGCGCGAATCTCACGGCTTTTCTCTTGTCTGTTTTCCTATCGGTTTGGATGACGCCTTTTATT
GTCAAAACGCTCGGTGTCGA

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIPLVSIIVPMYNVEPFIEECIDSLLRQTLSDIEIILVNDGTPDRSGEIAEDYAKRDARIRVIHQANGGLSSARNTGIKA
ARGTYIGFVDGDDYVSSAMFQRLTEEAEQNQLDIVGCGFYKQSSDRRTYVPPQLEANRVLTKPEMTEQLKHAHETRFIWY
VWRYLYRRELFERANLLFDEDIRFAEDSPFNLSAFREAERVKMLDEGLYIYRENPNSLTEIPYKPAMDEHLQKQYQAKIA
FYNHYGLAGACKEDLNVYICRHQLPMLLANACASPNSPKDIKKKIRQILSYDMVRQAVRHTPFQHEKLLRGERLVLALCK
WRLTFLIKLFFEQRGTMKGSAKQA

Sequences:

>Translated_344_residues
MIPLVSIIVPMYNVEPFIEECIDSLLRQTLSDIEIILVNDGTPDRSGEIAEDYAKRDARIRVIHQANGGLSSARNTGIKA
ARGTYIGFVDGDDYVSSAMFQRLTEEAEQNQLDIVGCGFYKQSSDRRTYVPPQLEANRVLTKPEMTEQLKHAHETRFIWY
VWRYLYRRELFERANLLFDEDIRFAEDSPFNLSAFREAERVKMLDEGLYIYRENPNSLTEIPYKPAMDEHLQKQYQAKIA
FYNHYGLAGACKEDLNVYICRHQLPMLLANACASPNSPKDIKKKIRQILSYDMVRQAVRHTPFQHEKLLRGERLVLALCK
WRLTFLIKLFFEQRGTMKGSAKQA
>Mature_344_residues
MIPLVSIIVPMYNVEPFIEECIDSLLRQTLSDIEIILVNDGTPDRSGEIAEDYAKRDARIRVIHQANGGLSSARNTGIKA
ARGTYIGFVDGDDYVSSAMFQRLTEEAEQNQLDIVGCGFYKQSSDRRTYVPPQLEANRVLTKPEMTEQLKHAHETRFIWY
VWRYLYRRELFERANLLFDEDIRFAEDSPFNLSAFREAERVKMLDEGLYIYRENPNSLTEIPYKPAMDEHLQKQYQAKIA
FYNHYGLAGACKEDLNVYICRHQLPMLLANACASPNSPKDIKKKIRQILSYDMVRQAVRHTPFQHEKLLRGERLVLALCK
WRLTFLIKLFFEQRGTMKGSAKQA

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Homo sapiens, GI57770468, Length=226, Percent_Identity=27.8761061946903, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790044, Length=240, Percent_Identity=31.6666666666667, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787259, Length=91, Percent_Identity=41.7582417582418, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSJ_BACSU (P71059)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   A70037
- RefSeq:   NP_391308.1
- ProteinModelPortal:   P71059
- SMR:   P71059
- EnsemblBacteria:   EBBACT00000002697
- GeneID:   937131
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34280
- NMPDR:   fig|224308.1.peg.3434
- GenoList:   BSU34280
- GeneTree:   EBGT00050000000531
- HOGENOM:   HBG338333
- OMA:   FIWYVWR
- PhylomeDB:   P71059
- ProtClustDB:   CLSK887908
- BioCyc:   BSUB:BSU34280-MONOMER
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: 2.-.-.- [C]

Molecular weight: Translated: 39945; Mature: 39945

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLVSIIVPMYNVEPFIEECIDSLLRQTLSDIEIILVNDGTPDRSGEIAEDYAKRDARI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCCEE
RVIHQANGGLSSARNTGIKAARGTYIGFVDGDDYVSSAMFQRLTEEAEQNQLDIVGCGFY
EEEEECCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCE
KQSSDRRTYVPPQLEANRVLTKPEMTEQLKHAHETRFIWYVWRYLYRRELFERANLLFDE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DIRFAEDSPFNLSAFREAERVKMLDEGLYIYRENPNSLTEIPYKPAMDEHLQKQYQAKIA
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHE
FYNHYGLAGACKEDLNVYICRHQLPMLLANACASPNSPKDIKKKIRQILSYDMVRQAVRH
EEECCCCCHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TPFQHEKLLRGERLVLALCKWRLTFLIKLFFEQRGTMKGSAKQA
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIPLVSIIVPMYNVEPFIEECIDSLLRQTLSDIEIILVNDGTPDRSGEIAEDYAKRDARI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCCEE
RVIHQANGGLSSARNTGIKAARGTYIGFVDGDDYVSSAMFQRLTEEAEQNQLDIVGCGFY
EEEEECCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCE
KQSSDRRTYVPPQLEANRVLTKPEMTEQLKHAHETRFIWYVWRYLYRRELFERANLLFDE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DIRFAEDSPFNLSAFREAERVKMLDEGLYIYRENPNSLTEIPYKPAMDEHLQKQYQAKIA
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHE
FYNHYGLAGACKEDLNVYICRHQLPMLLANACASPNSPKDIKKKIRQILSYDMVRQAVRH
EEECCCCCHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TPFQHEKLLRGERLVLALCKWRLTFLIKLFFEQRGTMKGSAKQA
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377