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The map label for this gene is epsM

Identifier: 16080477

GI number: 16080477

Start: 3516233

End: 3516883

Strand: Reverse

Name: epsM

Synonym: BSU34240

Alternate gene names: 16080477

Gene position: 3516883-3516233 (Counterclockwise)

Preceding gene: 16080478

Following gene: 50812296

Centisome position: 83.43

GC content: 54.69

Gene sequence:

>651_bases
ATGAAAAATGTGGCCATTGTGGGTGACGGCGGTCACGGAAAGGTGATCAGAGAGCTGATAAACGCCCGCTCAGATACGCG
CTTAGCCGCGGTGCTGGATGATAAATTCAAAACGTTCGAAGGCGGAAAAGAATGGTACACAGGACCGCCGAAAGCCGTTA
CTGAACTGCGCAGGCTCATTCCTGATGTGCTGTTTCTGATTGCTGTTGGGAATAACAGTGTCAGAAAACAGCTGGCGGAG
CGACTGGGACTGGGGAAAGATGATTTTATTACATTGATTCACCCGTCAGCCATCGTCAGCAAGTCGGCTGTCATTGGGGA
AGGGACAGTGATTATGGCGGGCGCGATCATTCAGGCGGATGCGCGCATCGGCGCCCACTGCATCATCAATACGGGTGCAG
TGGCAGAGCACGACAATCAAATCAGCGATTACGTTCATCTGTCCCCGCGTGCCACGCTGTCAGGAGCGGTTTCCGTTCAG
GAAGGCGCTCACGTCGGAACCGGTGCATCCGTCATACCGCAGATCATAATCGGGGCTTGGAGCATTGTCGGAGCCGGCTC
CGCGGTGATCCGTTCCATACCGGACAGGGTAACGGCGGCCGGTGCTCCGGCACGCATCATTTCTTCCATTCAAACATCAA
ACAAAGGATGA

Upstream 100 bases:

>100_bases
TTTTATGTTTGACGGTGCGAAAGGTCCTTGTTTCAGAAGGGATTCAGCAAACCAATCATGTGACCGCGGAACGGTTTACC
GGAAGCGGAGATGTGTCCTC

Downstream 100 bases:

>100_bases
TTCTATGCATAAAAAAATCTACTTATCTCCCCCTCATATGAGCGGCAGGGAGCAGCACTATATTTCAGAAGCCTTTCGCT
CAAACTGGATTGCGCCGCTT

Product: O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLIPDVLFLIAVGNNSVRKQLAE
RLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQADARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQ
EGAHVGTGASVIPQIIIGAWSIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG

Sequences:

>Translated_216_residues
MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLIPDVLFLIAVGNNSVRKQLAE
RLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQADARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQ
EGAHVGTGASVIPQIIIGAWSIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG
>Mature_216_residues
MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLIPDVLFLIAVGNNSVRKQLAE
RLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQADARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQ
EGAHVGTGASVIPQIIIGAWSIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSM_BACSU (P71063)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   E70037
- RefSeq:   NP_391304.1
- HSSP:   P43886
- ProteinModelPortal:   P71063
- SMR:   P71063
- EnsemblBacteria:   EBBACT00000003163
- GeneID:   936372
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34240
- NMPDR:   fig|224308.1.peg.3430
- GenoList:   BSU34240
- GeneTree:   EBGT00070000031764
- HOGENOM:   HBG703696
- OMA:   DYVHLSP
- ProtClustDB:   CLSK887906
- BioCyc:   BSUB:BSU34240-MONOMER
- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR03570

Pfam domain/function: SSF51161 Trimer_LpxA_like

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22483; Mature: 22483

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLI
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
PDVLFLIAVGNNSVRKQLAERLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQAD
HHHHEEHEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCEECCCCEEEEEEEEEEC
ARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQEGAHVGTGASVIPQIIIGAW
CCCCCEEEEECCCCCCCCCCHHHEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
SIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG
HHHCCCHHHHHHCCHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLI
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
PDVLFLIAVGNNSVRKQLAERLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQAD
HHHHEEHEECCHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCEECCCCEEEEEEEEEEC
ARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQEGAHVGTGASVIPQIIIGAW
CCCCCEEEEECCCCCCCCCCHHHEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
SIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG
HHHCCCHHHHHHCCHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377