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The map label for this gene is pgk

Identifier: 16080446

GI number: 16080446

Start: 3480197

End: 3481381

Strand: Reverse

Name: pgk

Synonym: BSU33930

Alternate gene names: 16080446

Gene position: 3481381-3480197 (Counterclockwise)

Preceding gene: 16080447

Following gene: 16080445

Centisome position: 82.58

GC content: 46.58

Gene sequence:

>1185_bases
ATGAATAAAAAAACTCTCAAAGACATCGACGTAAAAGGCAAAGTTGTATTCTGCCGCGTTGACTTTAACGTTCCAATGAA
AGACGGGGAAGTAACAGACGATACACGTATCCGTGCTGCGCTTCCAACAATCAAACACCTTGCAGACCAAGGCGCAAAAG
TCCTTCTTGCGAGCCACTTAGGCCGCCCGAAAGGCGAAGTGGTTGAGGAGCTTCGTTTAACTCCTGTCGCTGCACGTCTC
GGCGAACTGCTTGGCAAAGAAGTGAAAAAAGCGGATGAAGCATACGGCGATGCTGTAAAAGCACAAATTTCTGAGATGAA
GGACGGAGACGTTCTTGTATTGGAAAACGTACGTTTCTATCCTGGTGAAGAGAAAAATGATCCTGAGCTTGCAAAAGCAT
TTGCTGAGCTTGCAGATGTATATGTAAATGACGCATTCGGTGCTGCCCACCGTGCTCACGCATCTACAGCTGGAATTGCC
GAGCATCTGCCAGCAGTTGCAGGTTTCTTAATGGAAAAAGAGCTTGATGTACTTGGAAAAGCGGTTTCCAATCCTGACCG
CCCGTTCACAGCGATCATCGGCGGAGCGAAAGTAAAAGACAAAATCGGTGTAATCGAAAGTCTTCTTGATAAAGTAGACA
ACCTGATCATCGGCGGAGGTCTTGCTTATACTTTCGTAAAAGCGCTTGGCTATGAAGTCGGGAAATCTCTTCTTGAAGAG
GATAAAATCGAGCTTGCGAAATCATTTATGGACCGCGCTAAAGAAAAAGGCGTTAACTTCTACATGCCTGAAGATGTACT
CGTTGCAGATGATTTCTCTAACGATGCAAACGTGAAAATTGTGCCGATCTCTGAAATCCCTAGTGATTTAGAAGCAATCG
ACATTGGTACGAAAACACGCGAAACGTATGCTGACGTCATCAAAAACAGCAAACTTGTCGTGTGGAACGGACCAATGGGC
GTATTCGAAATCGACTTGTTCGCTCAAGGAACAAAAGCGGTTGCGGAAGCATTGGCAGAGGCGAAAGATACATACTCTGT
CATCGGCGGAGGAGACTCTGCGGCAGCGGTTGAAAAATTCGGCCTTGCTGACAAAATGAGCCACATCTCAACAGGCGGCG
GCGCATCCCTTGAGTTTATGGAAGGCAAAGAGCTTCCAGGGGTAGCTGCACTGAACGATAAATAA

Upstream 100 bases:

>100_bases
AAAAAGACTGCGATCTGCCCTAGCGGAGTTATCGCTTGAACAGACGGTTTCACGTTTTTCGTGCTGTATGCAAACCATTT
TGAAGGAGGATCTCCTAGGC

Downstream 100 bases:

>100_bases
TCTCAAAACTGCTATAAGGAAGTGGAACAGATGAGAAAACCAATTATCGCCGGTAACTGGAAAATGAACAAAACACTCGG
CGAAGCTGTCAGCTTCGTTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHLGRPKGEVVEELRLTPVAARL
GELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFYPGEEKNDPELAKAFAELADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELDVLGKAVSNPDRPFTAIIGGAKVKDKIGVIESLLDKVDNLIIGGGLAYTFVKALGYEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPEDVLVADDFSNDANVKIVPISEIPSDLEAIDIGTKTRETYADVIKNSKLVVWNGPMG
VFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVAALNDK

Sequences:

>Translated_394_residues
MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHLGRPKGEVVEELRLTPVAARL
GELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFYPGEEKNDPELAKAFAELADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELDVLGKAVSNPDRPFTAIIGGAKVKDKIGVIESLLDKVDNLIIGGGLAYTFVKALGYEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPEDVLVADDFSNDANVKIVPISEIPSDLEAIDIGTKTRETYADVIKNSKLVVWNGPMG
VFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVAALNDK
>Mature_394_residues
MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHLGRPKGEVVEELRLTPVAARL
GELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFYPGEEKNDPELAKAFAELADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELDVLGKAVSNPDRPFTAIIGGAKVKDKIGVIESLLDKVDNLIIGGGLAYTFVKALGYEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPEDVLVADDFSNDANVKIVPISEIPSDLEAIDIGTKTRETYADVIKNSKLVVWNGPMG
VFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVAALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=47.2154963680387, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=46.3768115942029, Blast_Score=348, Evalue=7e-96,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=47.7272727272727, Blast_Score=344, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=46.6019417475728, Blast_Score=353, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=48.18401937046, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=46.6183574879227, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=45.7420924574209, Blast_Score=328, Evalue=3e-90,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42191; Mature: 42191

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPKGEVVEELRLTPVAARLGELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEC
PGEEKNDPELAKAFAELADVYVNDAFGAAHRAHASTAGIAEHLPAVAGFLMEKELDVLGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSNPDRPFTAIIGGAKVKDKIGVIESLLDKVDNLIIGGGLAYTFVKALGYEVGKSLLEE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHH
DKIELAKSFMDRAKEKGVNFYMPEDVLVADDFSNDANVKIVPISEIPSDLEAIDIGTKTR
HHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCEEEEEHHHCCCCHHEEECCCCHH
ETYADVIKNSKLVVWNGPMGVFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKF
HHHHHHHCCCCEEEEECCCCEEEEEEHHCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
GLADKMSHISTGGGASLEFMEGKELPGVAALNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPKGEVVEELRLTPVAARLGELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEC
PGEEKNDPELAKAFAELADVYVNDAFGAAHRAHASTAGIAEHLPAVAGFLMEKELDVLGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSNPDRPFTAIIGGAKVKDKIGVIESLLDKVDNLIIGGGLAYTFVKALGYEVGKSLLEE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHH
DKIELAKSFMDRAKEKGVNFYMPEDVLVADDFSNDANVKIVPISEIPSDLEAIDIGTKTR
HHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCEEEEEHHHCCCCHHEEECCCCHH
ETYADVIKNSKLVVWNGPMGVFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKF
HHHHHHHCCCCEEEEECCCCEEEEEEHHCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
GLADKMSHISTGGGASLEFMEGKELPGVAALNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA