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The map label for this gene is gapA

Identifier: 16080447

GI number: 16080447

Start: 3481698

End: 3482705

Strand: Reverse

Name: gapA

Synonym: BSU33940

Alternate gene names: 16080447

Gene position: 3482705-3481698 (Counterclockwise)

Preceding gene: 16080448

Following gene: 16080446

Centisome position: 82.61

GC content: 43.55

Gene sequence:

>1008_bases
ATGGCAGTAAAAGTCGGTATTAACGGTTTTGGTCGTATTGGACGTAACGTATTCCGCGCAGCATTAAACAATCCTGAAGT
TGAGGTAGTAGCGGTTAACGATTTAACAGATGCTAACATGCTGGCTCACCTTTTACAATATGATTCTGTACACGGAAAAT
TAGACGCTGAAGTTTCAGTTGACGGTAACAACCTTGTTGTTAACGGCAAAACAATTGAAGTTTCTGCAGAACGCGATCCT
GCTAAACTTAGCTGGGGCAAACAAGGCGTTGAAATCGTAGTTGAATCTACTGGTTTCTTCACAAAACGCGCAGACGCTGC
GAAACACTTAGAAGCTGGCGCGAAAAAAGTAATCATCTCTGCTCCTGCTAACGAAGAAGATATCACAATCGTTATGGGTG
TTAACGAAGATAAATACGATGCGGCTAACCACGATGTTATCTCTAACGCATCTTGCACAACAAACTGCCTTGCGCCGTTT
GCAAAAGTACTTAACGATAAATTCGGCATCAAACGCGGTATGATGACAACTGTTCACTCTTACACAAACGATCAGCAAAT
CCTTGATCTTCCGCACAAAGACTACCGTCGTGCGCGTGCAGCAGCTGAAAACATCATCCCAACATCAACTGGTGCTGCTA
AAGCAGTTTCTCTAGTTCTTCCTGAACTAAAAGGCAAACTGAACGGTGGAGCAATGCGTGTTCCAACTCCAAACGTTTCT
CTAGTTGACTTGGTTGCTGAACTGAACCAAGAAGTAACAGCTGAAGAAGTAAACGCAGCTCTTAAAGAAGCGGCTGAAGG
CGACCTTAAAGGAATCCTTGGCTACAGCGAAGAGCCATTAGTTTCTGGCGACTACAACGGAAACAAAAACTCTTCTACAA
TCGATGCTCTTTCTACAATGGTTATGGAAGGCAGCATGGTAAAAGTAATCTCTTGGTACGATAACGAAAGCGGCTACTCT
AACCGCGTTGTTGACCTTGCAGCTTACATCGCAAAAAAAGGTCTTTAA

Upstream 100 bases:

>100_bases
ACGGTTCTCGTCACAGACGAAGGAGCCGCAAAGAAGTTATTAAGGGATGAATAATCCCTCAATATAAATATCTCTCACTT
ATTTAAAGGAGGAAACAATC

Downstream 100 bases:

>100_bases
TTTATAGCTGAAAAAGGACCTGACTTGGTTCTTTCGAATAGAAGCGCTATAATGAAAGCGGACAAGGGAAGGGGACGGAC
TCCCTTTCCCTTTTTCCATG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD-dependent glyceraldehyde-3-phosphate dehydrogenase; GAPDH

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAVKVGINGFGRIGRNVFRAALNNPEVEVVAVNDLTDANMLAHLLQYDSVHGKLDAEVSVDGNNLVVNGKTIEVSAERDP
AKLSWGKQGVEIVVESTGFFTKRADAAKHLEAGAKKVIISAPANEEDITIVMGVNEDKYDAANHDVISNASCTTNCLAPF
AKVLNDKFGIKRGMMTTVHSYTNDQQILDLPHKDYRRARAAAENIIPTSTGAAKAVSLVLPELKGKLNGGAMRVPTPNVS
LVDLVAELNQEVTAEEVNAALKEAAEGDLKGILGYSEEPLVSGDYNGNKNSSTIDALSTMVMEGSMVKVISWYDNESGYS
NRVVDLAAYIAKKGL

Sequences:

>Translated_335_residues
MAVKVGINGFGRIGRNVFRAALNNPEVEVVAVNDLTDANMLAHLLQYDSVHGKLDAEVSVDGNNLVVNGKTIEVSAERDP
AKLSWGKQGVEIVVESTGFFTKRADAAKHLEAGAKKVIISAPANEEDITIVMGVNEDKYDAANHDVISNASCTTNCLAPF
AKVLNDKFGIKRGMMTTVHSYTNDQQILDLPHKDYRRARAAAENIIPTSTGAAKAVSLVLPELKGKLNGGAMRVPTPNVS
LVDLVAELNQEVTAEEVNAALKEAAEGDLKGILGYSEEPLVSGDYNGNKNSSTIDALSTMVMEGSMVKVISWYDNESGYS
NRVVDLAAYIAKKGL
>Mature_334_residues
AVKVGINGFGRIGRNVFRAALNNPEVEVVAVNDLTDANMLAHLLQYDSVHGKLDAEVSVDGNNLVVNGKTIEVSAERDPA
KLSWGKQGVEIVVESTGFFTKRADAAKHLEAGAKKVIISAPANEEDITIVMGVNEDKYDAANHDVISNASCTTNCLAPFA
KVLNDKFGIKRGMMTTVHSYTNDQQILDLPHKDYRRARAAAENIIPTSTGAAKAVSLVLPELKGKLNGGAMRVPTPNVSL
VDLVAELNQEVTAEEVNAALKEAAEGDLKGILGYSEEPLVSGDYNGNKNSSTIDALSTMVMEGSMVKVISWYDNESGYSN
RVVDLAAYIAKKGL

Specific function: More active in catabolism

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=54.6546546546547, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=49.2447129909366, Blast_Score=320, Evalue=9e-88,
Organism=Escherichia coli, GI1788079, Length=333, Percent_Identity=56.1561561561562, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=44.0729483282675, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=52.5222551928783, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=53.8690476190476, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=53.8690476190476, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=52.5222551928783, Blast_Score=344, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=54.8192771084337, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=53.9156626506024, Blast_Score=356, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=52.1084337349398, Blast_Score=347, Evalue=1e-96,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=53.6144578313253, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=53.6144578313253, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=53.6144578313253, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=53.6144578313253, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI19922412, Length=327, Percent_Identity=50.7645259938838, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P1_BACSU (P09124)

Other databases:

- EMBL:   X13011
- EMBL:   AL009126
- PIR:   S02754
- RefSeq:   NP_391274.1
- ProteinModelPortal:   P09124
- SMR:   P09124
- PhosSite:   P09124
- EnsemblBacteria:   EBBACT00000003267
- GeneID:   938627
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33940
- NMPDR:   fig|224308.1.peg.3400
- GenoList:   BSU33940
- GeneTree:   EBGT00050000001163
- HOGENOM:   HBG571736
- OMA:   NTDEKAS
- PhylomeDB:   P09124
- ProtClustDB:   CLSK873643
- BioCyc:   BSUB:BSU33940-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 35833; Mature: 35702

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 152-152 BINDING 34-34 BINDING 78-78 BINDING 182-182 BINDING 197-197 BINDING 233-233 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKVGINGFGRIGRNVFRAALNNPEVEVVAVNDLTDANMLAHLLQYDSVHGKLDAEVSV
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEE
DGNNLVVNGKTIEVSAERDPAKLSWGKQGVEIVVESTGFFTKRADAAKHLEAGAKKVIIS
CCCEEEEECEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEE
APANEEDITIVMGVNEDKYDAANHDVISNASCTTNCLAPFAKVLNDKFGIKRGMMTTVHS
CCCCCCCEEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCHHHCHHHHHHH
YTNDQQILDLPHKDYRRARAAAENIIPTSTGAAKAVSLVLPELKGKLNGGAMRVPTPNVS
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCH
LVDLVAELNQEVTAEEVNAALKEAAEGDLKGILGYSEEPLVSGDYNGNKNSSTIDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VMEGSMVKVISWYDNESGYSNRVVDLAAYIAKKGL
HHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVKVGINGFGRIGRNVFRAALNNPEVEVVAVNDLTDANMLAHLLQYDSVHGKLDAEVSV
EEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEE
DGNNLVVNGKTIEVSAERDPAKLSWGKQGVEIVVESTGFFTKRADAAKHLEAGAKKVIIS
CCCEEEEECEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEE
APANEEDITIVMGVNEDKYDAANHDVISNASCTTNCLAPFAKVLNDKFGIKRGMMTTVHS
CCCCCCCEEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCHHHCHHHHHHH
YTNDQQILDLPHKDYRRARAAAENIIPTSTGAAKAVSLVLPELKGKLNGGAMRVPTPNVS
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCH
LVDLVAELNQEVTAEEVNAALKEAAEGDLKGILGYSEEPLVSGDYNGNKNSSTIDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VMEGSMVKVISWYDNESGYSNRVVDLAAYIAKKGL
HHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2493629; 9384377; 8755892; 10658653; 10799476