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The map label for this gene is opuBB

Identifier: 16080425

GI number: 16080425

Start: 3461435

End: 3462088

Strand: Reverse

Name: opuBB

Synonym: BSU33720

Alternate gene names: 16080425

Gene position: 3462088-3461435 (Counterclockwise)

Preceding gene: 16080426

Following gene: 16080424

Centisome position: 82.13

GC content: 48.32

Gene sequence:

>654_bases
ATGCATCATATTGTTCAATTTTTGCAAACCAATGGGGGAGAGCTTCTTTATAAAACATACGAGCATATTACGATATCGCT
CATTGCTGTGATCTTAGGTGTGCTGGTTGCCGTCCCCCTCGGGGTTGTGCTTACCAGAATGAAAAAAGGAGCCGGCACCA
TTATCGGCATTGTCAATATCATTCAGACGCTGCCGAGTCTGGCCATCCTCGCTTTTTTTATCCCGCTCCTTGGTGTAGGG
AAGGTTCCGGCCATTGTGGCTTTGTTTTTCTATTCCGTATTGCCGATTCTTCGCAACACGTATACCGGCATTCGCGGCGT
CAACAAAAATCTGCTCGAGTCTGGTAAAGGAATAGGAATGACGCCGGCTGAACAAGTCCGCTTGGTGGAGCTGCCGCTTG
CCGCACCAGTGATCATGGCGGGAATCAGAACATCGACGATTTACTTAATCGGCTGGGCCACGCTTGCTTCCTTTATCGGA
GGCGGCGGATTGGGTGATTACATTTTTATCGGACTGAATTTATATCAGCCGGAATATATCATCGGGGGCGCGGTACCTGT
CACGATATTGGCAATTGTGATTGATTATGTGCTGGCAGTTGCTGAACGAAAACTGACACCTGCCGGCATGCAGAGATTGA
AGGAATTGTCATAA

Upstream 100 bases:

>100_bases
GGAATTGTGACGAGGGCAAGTCTGGTCGACATCGTGTATGACTCCCTCTGGGGAGAAGAAAAGCAGCTCGCAGCATTGTC
ATAGGGAGGGGCGATAAGTC

Downstream 100 bases:

>100_bases
GGAGGCGGCTCATGAAAAGAAAATATCTCAAATTAATGATAGGTTTAGCACTTGCGGCAACGCTGACGCTGAGCGGCTGT
TCGCTTCCTGGTCTCAGCGC

Product: choline ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNIIQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIRGVNKNLLESGKGIGMTPAEQVRLVELPLAAPVIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIVIDYVLAVAERKLTPAGMQRLKELS

Sequences:

>Translated_217_residues
MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNIIQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIRGVNKNLLESGKGIGMTPAEQVRLVELPLAAPVIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIVIDYVLAVAERKLTPAGMQRLKELS
>Mature_217_residues
MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNIIQTLPSLAILAFFIPLLGVG
KVPAIVALFFYSVLPILRNTYTGIRGVNKNLLESGKGIGMTPAEQVRLVELPLAAPVIMAGIRTSTIYLIGWATLASFIG
GGGLGDYIFIGLNLYQPEYIIGGAVPVTILAIVIDYVLAVAERKLTPAGMQRLKELS

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for choline; probably responsible for the translocation of the substrate across the membrane

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1788449, Length=134, Percent_Identity=48.5074626865672, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.4607329842932, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789033, Length=179, Percent_Identity=31.2849162011173, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPUBB_BACSU (Q45461)

Other databases:

- EMBL:   U38418
- EMBL:   AF008930
- EMBL:   AL009126
- PIR:   H69669
- RefSeq:   NP_391252.1
- ProteinModelPortal:   Q45461
- SMR:   Q45461
- EnsemblBacteria:   EBBACT00000003194
- GeneID:   936225
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33720
- NMPDR:   fig|224308.1.peg.3378
- GenoList:   BSU33720
- GeneTree:   EBGT00070000031878
- HOGENOM:   HBG592565
- OMA:   WMVELPL
- PhylomeDB:   Q45461
- ProtClustDB:   CLSK885988
- BioCyc:   BSUB:BSU33720-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 23163; Mature: 23163

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11c9639c)-; HASH(0x1158d164)-; HASH(0x120bc6e0)-; HASH(0x12947218)-; HASH(0x124046e4)-; HASH(0x121fc948)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNI
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IQTLPSLAILAFFIPLLGVGKVPAIVALFFYSVLPILRNTYTGIRGVNKNLLESGKGIGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
TPAEQVRLVELPLAAPVIMAGIRTSTIYLIGWATLASFIGGGGLGDYIFIGLNLYQPEYI
CHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEE
IGGAVPVTILAIVIDYVLAVAERKLTPAGMQRLKELS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCH
>Mature Secondary Structure
MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNI
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IQTLPSLAILAFFIPLLGVGKVPAIVALFFYSVLPILRNTYTGIRGVNKNLLESGKGIGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
TPAEQVRLVELPLAAPVIMAGIRTSTIYLIGWATLASFIGGGGLGDYIFIGLNLYQPEYI
CHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEE
IGGAVPVTILAIVIDYVLAVAERKLTPAGMQRLKELS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7592481; 10216873; 9384377