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The map label for this gene is opuBC

Identifier: 16080424

GI number: 16080424

Start: 3460503

End: 3461423

Strand: Reverse

Name: opuBC

Synonym: BSU33710

Alternate gene names: 16080424

Gene position: 3461423-3460503 (Counterclockwise)

Preceding gene: 16080425

Following gene: 16080423

Centisome position: 82.11

GC content: 46.25

Gene sequence:

>921_bases
ATGAAAAGAAAATATCTCAAATTAATGATAGGTTTAGCACTTGCGGCAACGCTGACGCTGAGCGGCTGTTCGCTTCCTGG
TCTCAGCGCTGCTGCAGACCAGACCATTAAAATCGGCGCGCAAAGCATGAGCGAATCAGAAATCATCGCCAGCATGCTTG
GCCAGTTGATCGAACACCATACTGATCTCAAAACAACAACGATTAAAAATCTTGGCTCCAACGCGGTGCAGCAGCAGGCT
TTAATGAACGGGGAAATCGACATTGCGGCCACAAGATATACGGGAGACGCGCTGACAGGCACGTTGAGAATGGAACCTGA
AAAAGATCCGGACAAAGCGCTGGCGCTTACTCAGCGGGAGTTTAAAAAAAGATATGATTTAAAGTGGTATGACTCCTACG
GGTTTGATAATACATATGCCTTTACAGTCAGCAAGGAGCTGGCCGACCAATACCATTTAGAGACCGTATCAGACGTTAAA
AAATGGGCGCCGCAATTAAAGCTGGGCGTTGATAACTATTGGATGAAGCTCAAAGGAAACGGCTATCAGGATTTTACGAA
AACCTACGGCATGACATTCGGCGGCACGTATCCGATGCAGATCGGCCTTGTGTACGACGCGGTGAAAAGCGGAAAAATGG
ACATTGTGCTTGCGTATTCAACCGATGGAAGAATCAAGTCCTATGGTCTCAAAATGCTAAAGGATGATAAACAATTTTTC
CCGCCGTACGACTGCTCTCCGGTTGTTCCGGAAAAGGTGCTCAAAGAACATCCTGAACTTGAAGGCATCATCAAGAAAAT
GCTCGGAAAAATCGACACAGCCACGATGCAGGAGCTTAACTATGAAGTCGACGGCAATCTCAAAGAACCGTCTGTCGTTG
CCAAGGAATATTTAGAAAAACATCGCTATTTCGAATCGTGA

Upstream 100 bases:

>100_bases
TATTGGCAATTGTGATTGATTATGTGCTGGCAGTTGCTGAACGAAAACTGACACCTGCCGGCATGCAGAGATTGAAGGAA
TTGTCATAAGGAGGCGGCTC

Downstream 100 bases:

>100_bases
AAGGGGGAAGAGGTCAATGAACGTGCTTGAACAGTTAATGACGTATTACGCGCAGAATGGCAGTTACGTCATGGATGAAT
TCGGCCGCCACTTTCTCATG

Product: choline ABC transporter choline-binding lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKRKYLKLMIGLALAATLTLSGCSLPGLSAAADQTIKIGAQSMSESEIIASMLGQLIEHHTDLKTTTIKNLGSNAVQQQA
LMNGEIDIAATRYTGDALTGTLRMEPEKDPDKALALTQREFKKRYDLKWYDSYGFDNTYAFTVSKELADQYHLETVSDVK
KWAPQLKLGVDNYWMKLKGNGYQDFTKTYGMTFGGTYPMQIGLVYDAVKSGKMDIVLAYSTDGRIKSYGLKMLKDDKQFF
PPYDCSPVVPEKVLKEHPELEGIIKKMLGKIDTATMQELNYEVDGNLKEPSVVAKEYLEKHRYFES

Sequences:

>Translated_306_residues
MKRKYLKLMIGLALAATLTLSGCSLPGLSAAADQTIKIGAQSMSESEIIASMLGQLIEHHTDLKTTTIKNLGSNAVQQQA
LMNGEIDIAATRYTGDALTGTLRMEPEKDPDKALALTQREFKKRYDLKWYDSYGFDNTYAFTVSKELADQYHLETVSDVK
KWAPQLKLGVDNYWMKLKGNGYQDFTKTYGMTFGGTYPMQIGLVYDAVKSGKMDIVLAYSTDGRIKSYGLKMLKDDKQFF
PPYDCSPVVPEKVLKEHPELEGIIKKMLGKIDTATMQELNYEVDGNLKEPSVVAKEYLEKHRYFES
>Mature_306_residues
MKRKYLKLMIGLALAATLTLSGCSLPGLSAAADQTIKIGAQSMSESEIIASMLGQLIEHHTDLKTTTIKNLGSNAVQQQA
LMNGEIDIAATRYTGDALTGTLRMEPEKDPDKALALTQREFKKRYDLKWYDSYGFDNTYAFTVSKELADQYHLETVSDVK
KWAPQLKLGVDNYWMKLKGNGYQDFTKTYGMTFGGTYPMQIGLVYDAVKSGKMDIVLAYSTDGRIKSYGLKMLKDDKQFF
PPYDCSPVVPEKVLKEHPELEGIIKKMLGKIDTATMQELNYEVDGNLKEPSVVAKEYLEKHRYFES

Specific function: Member of a high affinity multicomponent binding- protein-dependent transport system for choline

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788452, Length=313, Percent_Identity=25.5591054313099, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPUBC_BACSU (Q45462)

Other databases:

- EMBL:   U38418
- EMBL:   AF008930
- EMBL:   AL009126
- PIR:   A69670
- RefSeq:   NP_391251.1
- ProteinModelPortal:   Q45462
- SMR:   Q45462
- EnsemblBacteria:   EBBACT00000000908
- GeneID:   938475
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33710
- NMPDR:   fig|224308.1.peg.3377
- GenoList:   BSU33710
- GeneTree:   EBGT00070000031878
- HOGENOM:   HBG694086
- OMA:   PSEANNT
- PhylomeDB:   Q45462
- ProtClustDB:   CLSK885987
- BioCyc:   BSUB:BSU33710-MONOMER
- GO:   GO:0005488
- InterPro:   IPR007210

Pfam domain/function: PF04069 OpuAC

EC number: NA

Molecular weight: Translated: 34401; Mature: 34401

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKYLKLMIGLALAATLTLSGCSLPGLSAAADQTIKIGAQSMSESEIIASMLGQLIEHH
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHEECHHHCCHHHHHHHHHHHHHHHH
TDLKTTTIKNLGSNAVQQQALMNGEIDIAATRYTGDALTGTLRMEPEKDPDKALALTQRE
CCCHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
FKKRYDLKWYDSYGFDNTYAFTVSKELADQYHLETVSDVKKWAPQLKLGVDNYWMKLKGN
HHHHCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
GYQDFTKTYGMTFGGTYPMQIGLVYDAVKSGKMDIVLAYSTDGRIKSYGLKMLKDDKQFF
CCHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHCC
PPYDCSPVVPEKVLKEHPELEGIIKKMLGKIDTATMQELNYEVDGNLKEPSVVAKEYLEK
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCEEECCCCCCHHHHHHHHHHH
HRYFES
HHCCCC
>Mature Secondary Structure
MKRKYLKLMIGLALAATLTLSGCSLPGLSAAADQTIKIGAQSMSESEIIASMLGQLIEHH
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHEECHHHCCHHHHHHHHHHHHHHHH
TDLKTTTIKNLGSNAVQQQALMNGEIDIAATRYTGDALTGTLRMEPEKDPDKALALTQRE
CCCHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
FKKRYDLKWYDSYGFDNTYAFTVSKELADQYHLETVSDVKKWAPQLKLGVDNYWMKLKGN
HHHHCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
GYQDFTKTYGMTFGGTYPMQIGLVYDAVKSGKMDIVLAYSTDGRIKSYGLKMLKDDKQFF
CCHHHHHHCCCCCCCCCCEEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHCC
PPYDCSPVVPEKVLKEHPELEGIIKKMLGKIDTATMQELNYEVDGNLKEPSVVAKEYLEK
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCEEECCCCCCHHHHHHHHHHH
HRYFES
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7592481; 10216873; 9384377