Click here to switch to the map view.

The map label for this gene is liaR

Identifier: 16080361

GI number: 16080361

Start: 3394422

End: 3395057

Strand: Reverse

Name: liaR

Synonym: BSU33080

Alternate gene names: 16080361

Gene position: 3395057-3394422 (Counterclockwise)

Preceding gene: 16080362

Following gene: 255767753

Centisome position: 80.54

GC content: 48.74

Gene sequence:

>636_bases
GTGATTCGAGTATTATTGATTGATGATCATGAAATGGTCAGAATGGGGCTCGCGGCTTTTTTGGAGGCGCAGCCCGATAT
TGAAGTCATCGGCGAAGCATCGGACGGCAGCGAAGGTGTTCGGCTTGCTGTGGAACTGTCGCCTGATGTCATTTTAATGG
ACCTTGTCATGGAGGGCATGGATGGCATTGAAGCTACAAAGCAAATTTGCCGGGAGCTTTCCGACCCGAAAATTATTGTG
CTCACTAGCTTCATTGATGATGACAAAGTGTACCCGGTTATTGAAGCTGGCGCGCTCAGCTATCTGTTGAAAACCTCAAA
AGCGGCAGAAATCGCCGATGCCATCCGCGCCGCAAGCAAGGGAGAGCCGAAGCTGGAGTCAAAAGTGGCGGGAAAAGTAT
TATCCAGGCTGCGCCACTCAGGTGAAAACGCGCTCCCGCATGAATCGCTTACAAAACGGGAGCTCGAAATACTCTGCCTG
ATCGCAGAAGGAAAGACAAACAAAGAAATAGGCGAGGAACTGTTTATTACGATTAAAACAGTCAAAACACATATTACGAA
TATTTTATCAAAGCTGGATGTCAGTGACCGGACGCAGGCGGCGGTGTACGCACACCGAAATCATCTCGTGAATTAG

Upstream 100 bases:

>100_bases
GAAATCGGCGGTGTCGCCGAAGTGATTTCAGTAGAAGGAAAAGGCACTCAAATCGAAGTGAAGGTCCCGATTTTTCCGGA
AGAAAAAGGAGAGAACGAAC

Downstream 100 bases:

>100_bases
GCGGCTGCTATTTGTTTGCGCCTTTCGTTCCGAAGTCTCTGACAATCACATTGACCCGGTAGTCTATTTCCGCATTGCTG
AAATAATCATCATCCCATTT

Product: two-component response regulator YvqE responding to cell wall stress

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGMDGIEATKQICRELSDPKIIV
LTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASKGEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCL
IAEGKTNKEIGEELFITIKTVKTHITNILSKLDVSDRTQAAVYAHRNHLVN

Sequences:

>Translated_211_residues
MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGMDGIEATKQICRELSDPKIIV
LTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASKGEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCL
IAEGKTNKEIGEELFITIKTVKTHITNILSKLDVSDRTQAAVYAHRNHLVN
>Mature_211_residues
MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGMDGIEATKQICRELSDPKIIV
LTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASKGEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCL
IAEGKTNKEIGEELFITIKTVKTHITNILSKLDVSDRTQAAVYAHRNHLVN

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=37.7358490566038, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=39.3034825870647, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=36.7149758454106, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=30.8457711442786, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1788712, Length=213, Percent_Identity=26.2910798122066, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=38.4615384615385, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=25.6038647342995, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788546, Length=198, Percent_Identity=28.2828282828283, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787277, Length=169, Percent_Identity=29.585798816568, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIAR_BACSU (O32197)

Other databases:

- EMBL:   AJ223978
- EMBL:   AL009126
- PIR:   E70045
- RefSeq:   NP_391188.1
- ProteinModelPortal:   O32197
- SMR:   O32197
- EnsemblBacteria:   EBBACT00000002538
- GeneID:   935957
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33080
- NMPDR:   fig|224308.1.peg.3314
- GenoList:   BSU33080
- GeneTree:   EBGT00070000031892
- HOGENOM:   HBG753323
- OMA:   MVRMGLA
- PhylomeDB:   O32197
- ProtClustDB:   CLSK887857
- BioCyc:   BSUB:BSU33080-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 23125; Mature: 23125

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCC
DGIEATKQICRELSDPKIIVLTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASK
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCLIAEGKTNKEIGEELFITIKT
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCEEEEEEEECCCCCHHCCHHHEEHHHH
VKTHITNILSKLDVSDRTQAAVYAHRNHLVN
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCC
DGIEATKQICRELSDPKIIVLTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASK
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCLIAEGKTNKEIGEELFITIKT
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCEEEEEEEECCCCCHHCCHHHEEHHHH
VKTHITNILSKLDVSDRTQAAVYAHRNHLVN
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377