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The map label for this gene is liaS

Identifier: 16080362

GI number: 16080362

Start: 3395035

End: 3396117

Strand: Reverse

Name: liaS

Synonym: BSU33090

Alternate gene names: 16080362

Gene position: 3396117-3395035 (Counterclockwise)

Preceding gene: 16080363

Following gene: 16080361

Centisome position: 80.56

GC content: 47.46

Gene sequence:

>1083_bases
ATGAGAAAAAAAATGCTTGCCAGCCTCCAATGGCGCGCCATCCGCATGACAACGGGAATCAGCCTGCTCCTTTTTGTTTG
CCTGATTTCCTTTATGATGTTTTACTATCGGCTCGATCCGCTTGTTTTGCTGTCATCAAGCTGGTTCGGAATTCCGTTTA
TCCTGATTTTGCTTCTGATCAGCGTGACCGTCGGTTTCGCCTCAGGGTATATGTACGGCAACCGGTTGAAGACAAGGATT
GATACATTAATTGAATCCATTTTAACCTTTGAAAACGGCAATTTCGCTTATCGGATACCGCCGCTCGGTGATGATGAAAT
CGGCCTGGCTGCTGATCAGCTGAACGAAATGGCGAAGCGCGTGGAGCTTCAAGTCGCATCCCTCCAGAAACTTTCCAATG
AACGTGCGGAATGGCAGGCTCAAATGAAGAAGTCGGTTATCTCAGAAGAACGCCAGCGATTGGCCAGAGATCTTCATGAT
GCGGTCAGCCAGCAGCTCTTTGCCATATCGATGATGACATCAGCCGTGCTGGAACATGTCAAGGATGCTGATGACAAAAC
AGTCAAGCGGATCAGGATGGTCGAGCATATGGCAGGCGAAGCCCAAAATGAGATGAGGGCGCTGCTGCTCCATTTACGGC
CTGTTACCCTTGAAGGAAAAGGGCTGAAGGAGGGCCTTACGGAGCTTTTGGACGAGTTCCGAAAAAAGCAGCCGATTGAT
ATTGAGTGGGATATACAGGACACAGCGATATCCAAGGGTGTTGAAGACCACTTGTTCAGAATCGTGCAGGAGGCCCTTTC
AAACGTATTTAGACATTCAAAAGCGTCAAAAGTAACCGTGATTCTGGGCATAAAGAACAGCCAGCTCCGTCTGAAGGTGA
TTGATAATGGAAAAGGCTTTAAAATGGACCAGGTGAAAGCCTCCTCATACGGCTTGAATTCTATGAAAGAACGTGCAAGT
GAAATCGGCGGTGTCGCCGAAGTGATTTCAGTAGAAGGAAAAGGCACTCAAATCGAAGTGAAGGTCCCGATTTTTCCGGA
AGAAAAAGGAGAGAACGAACGTGATTCGAGTATTATTGATTGA

Upstream 100 bases:

>100_bases
GCACGAAGGTATATGCCGCGTCAACTGATTTTAGCGAGTCAAAGCGCCGGGTAAAAGTGTCCGTTTCCTTATTTATCGGT
GATGTGGATGTGAAGTACGT

Downstream 100 bases:

>100_bases
TGATCATGAAATGGTCAGAATGGGGCTCGCGGCTTTTTTGGAGGCGCAGCCCGATATTGAAGTCATCGGCGAAGCATCGG
ACGGCAGCGAAGGTGTTCGG

Product: two-component sensor histidine kinase YvqC sensing cell wall stress

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLISVTVGFASGYMYGNRLKTRI
DTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHD
AVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPID
IEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKERAS
EIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID

Sequences:

>Translated_360_residues
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLISVTVGFASGYMYGNRLKTRI
DTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHD
AVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPID
IEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKERAS
EIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID
>Mature_360_residues
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLISVTVGFASGYMYGNRLKTRI
DTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKRVELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHD
AVSQQLFAISMMTSAVLEHVKDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPID
IEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGFKMDQVKASSYGLNSMKERAS
EIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1787474, Length=212, Percent_Identity=28.3018867924528, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788812, Length=217, Percent_Identity=26.7281105990783, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082311, Length=221, Percent_Identity=29.8642533936652, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIAS_BACSU (O32198)

Other databases:

- EMBL:   AJ223978
- EMBL:   AL009126
- PIR:   F70045
- RefSeq:   NP_391189.1
- ProteinModelPortal:   O32198
- SMR:   O32198
- EnsemblBacteria:   EBBACT00000003398
- GeneID:   935967
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33090
- NMPDR:   fig|224308.1.peg.3315
- GenoList:   BSU33090
- GeneTree:   EBGT00070000031901
- HOGENOM:   HBG697489
- OMA:   IDIDWEI
- ProtClustDB:   CLSK872960
- BioCyc:   BSUB:BSU33090-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202
- Gene3D:   G3DSA:3.30.565.10
- PIRSF:   PIRSF037431
- SMART:   SM00304
- SMART:   SM00387

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3; SSF55874 ATP_bd_ATPase

EC number: =2.7.13.3

Molecular weight: Translated: 40705; Mature: 40705

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe6105b4)-; HASH(0x1188bc00)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCHHHHHHHHHH
SVTVGFASGYMYGNRLKTRIDTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
VELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPID
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCC
IEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGF
EEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEECCCCC
KMDQVKASSYGLNSMKERASEIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID
CHHHCCHHHCCHHHHHHHHHHHCCHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MRKKMLASLQWRAIRMTTGISLLLFVCLISFMMFYYRLDPLVLLSSSWFGIPFILILLLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCHHHHHHHHHH
SVTVGFASGYMYGNRLKTRIDTLIESILTFENGNFAYRIPPLGDDEIGLAADQLNEMAKR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
VELQVASLQKLSNERAEWQAQMKKSVISEERQRLARDLHDAVSQQLFAISMMTSAVLEHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDADDKTVKRIRMVEHMAGEAQNEMRALLLHLRPVTLEGKGLKEGLTELLDEFRKKQPID
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCC
IEWDIQDTAISKGVEDHLFRIVQEALSNVFRHSKASKVTVILGIKNSQLRLKVIDNGKGF
EEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEECCCCC
KMDQVKASSYGLNSMKERASEIGGVAEVISVEGKGTQIEVKVPIFPEEKGENERDSSIID
CHHHCCHHHCCHHHHHHHHHHHCCHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377