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The map label for this gene is pucD

Identifier: 16080301

GI number: 16080301

Start: 3336263

End: 3338500

Strand: Reverse

Name: pucD

Synonym: BSU32480

Alternate gene names: 16080301

Gene position: 3338500-3336263 (Counterclockwise)

Preceding gene: 16080302

Following gene: 16080300

Centisome position: 79.19

GC content: 51.3

Gene sequence:

>2238_bases
ATGATCATCAACAAACCGTCAAGAGTCAGGCCGGATGGAAGGGGTAAGGTCACAGGGGAATTGAAATATATGACCGATCT
CAGCTTTCCAGGAATGCTGTATGGCAAGGTGCTGAGAAGTGCTTATCCTCATGCAGAGATTGTGTCGGTTTGCACCATAA
AAGCTGAGAAAATGGAAGGCGTGCAGGCCGTCGTCACACATAAAGACGTTCCCGGCTTAAACCGATTTGGCATTGTCATC
CCTGATCAGCCGGTTTTATGTGAGGACAGGGTGCGGTATGTCGGGGATGCGATCGCCGCAGTTGCTGCGGAAACGGAAGA
AATCGCGGAAGCGGCGCTGGAGCTGATTCAAGTTGAGTATAAAGAGCTGGAAGTCATGGATTCACCAGAAAAAGCGCTTC
GGCCGAATGCGCAAAGACTGCATGAGGACGGGAATATCCTGCACCGCGCATTTTTTTCAAACGGTGATGTGGAAGAAGGG
TTTCAAGCATCTGACACAGTCTTTGAAGAAACCTATGAGCTGCCGCGGCAGATGCATACGTATATGGAAACGGAGGGAGG
TGTAGCCGTTCCGGAAGACGACGGGGGTTTTACGATGTATGCAGGAACCCAGCACGGCTATAAAGACCGTTTCCAGCTTG
CCCGTATTTTTGATATTCCTGAAGAGAAGATCAGAATTGTGTCAAGTCCGATGGGCGGCTCGTTCGGAGGGAAGGATGAG
CTGAATATTCAGCCGTATGCTGCGCTTTTGGCCCTGAAAAGCGGACGCCCTGTCAAAATTCATCAGACACGAAAGGAATC
TGTACGTTCTGGCATTAAGCGCCATCCGATGAAGATTACGATAAAAACGGGTGCAGATCATTCGGGAAATCTATTGGCGC
ACGACGTGAAAATTGTGGCGGACACAGGCGCTTACGCTACGCTCGGACCGGCTGTTCTTGATTTTTCTGTCGAGCATGCC
GCGGGGCCGTACCGTATTCCGAATATCCGGACTGAAGGAATATCAGTATTTACGAACAACGGGGTGGCGGGGGAATTTCG
GGGCTTTGGCGGCAACCAAATCACATTTGCACTCGAAACCCATCTCGATCGTCTCAGCGGCATGCTTGGCATCGACCCGC
TCGAGCTGAGAAGAAAAAATATCAGAAAACCGCATGACTTGGGGCCGCTTGAGCATCGAATTGCACCAACTGACGGAGCG
GCACAGGTGTTGAATGCCATATCTAAATCTCCCATTCTTAAAAAAACAAGCCGGAACTGCGGGTATCTGCAAAGAGGCAC
GGGAGCGGCAATTACAATGCACGGCGGCGGGCTGGGGTTTGGCCGAATGGATGCGGCAGGAGGCCGTTTGTCTCTTTCGA
GTGAAGGCAAAATCACTGCTTCGTTTGGATTTGAAGAATGCGGACAGGGGATTCTAGCAGCGATTGAACAGATTGTCATG
GAGGAGCTGGGTTGTGCCGCAGAGGATATTTCCATTGTTATTGGGGATACCGCAAAAGTGCCGAAGTCAGGCTCCTCCAC
AGCATCCCGCGGCACAAGCATGGTGTGGCACGCGATCCAGCGTTTGAAGAAGCCGTTTCTAGCTCAGTTGAAAAAACGGG
CGGCAGAATGGAGCGGCTGTTCAGCGGAAAATCTTATTCCCGGCGCTGCAGGCTTGCGTGACAAAAACACAAAGGCGCTC
GTGGTGACGTATAAGGAGCTGGCTGAAAAAGGCCCTTTGGCAGAGGAAACGGCCTTTGACTTTCCAACAACGCCTGATCC
TGTGGTGGGCGGCCATTTCCTCTACTCATTTGGAGCGGCTGCCGTTGAGGTGGAGGTAGATCTGTTAACAGGCGATGTCA
AATTGATAGATTGTGAGCACGCTATTGCGGCAGGTCCGGTTGTCAGTCCGCAGGGATATCGAGGTCAAATTGAAGGCGGC
GCTGCCATGGCACTCGGCTACACACTGATGGAGGAAGCGAAAATGACGGATGGCCGTTATGCTGCGGAAAATCTCGATCA
CTATTTGATTCCCGGAATCAAAGATGTTCCTGACATGAAGCTGATTGCAATAGAAGACTTAATGAAGGGAGACGTATATG
GACCGCGCGGTGTTGGTGAAATCGGAACAATCGCCATCACTCCGGCGATTGTAAAGGCAGTACATGATGCGGTCGGATGC
TGGATAAACAAGCTGCCAATTTCAAGAGAAGAGTTGCTTGAAGCGATCGACAGAAAGGGGCTGAAGCAATGGACATAA

Upstream 100 bases:

>100_bases
GCTGCCGTTTTCCTCAGATGTTTTTATGTCAGCGGCATACAGAAAAAAAGCCGCTGCCAACGTCATCATGGCAGAGCTGA
TGGCTGAGGGAGGGGAATAA

Downstream 100 bases:

>100_bases
AAGAGGCCGGGCCATTTCCTGTAAAAAAGGAACAGTTCCGGATGACCGTGAATGGTCAGGCGTGGGAGGTTGCTGCCGTT
CCTACGACACATCTGAGTGA

Product: xanthine dehydrogenase, substrate and molybdenum cofactor subunit

Products: NA

Alternate protein names: XDHase subunit D

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEGVQAVVTHKDVPGLNRFGIVI
PDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEYKELEVMDSPEKALRPNAQRLHEDGNILHRAFFSNGDVEEG
FQASDTVFEETYELPRQMHTYMETEGGVAVPEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE
LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVADTGAYATLGPAVLDFSVEHA
AGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALETHLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGA
AQVLNAISKSPILKKTSRNCGYLQRGTGAAITMHGGGLGFGRMDAAGGRLSLSSEGKITASFGFEECGQGILAAIEQIVM
EELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGCSAENLIPGAAGLRDKNTKAL
VVTYKELAEKGPLAEETAFDFPTTPDPVVGGHFLYSFGAAAVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGG
AAMALGYTLMEEAKMTDGRYAAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGC
WINKLPISREELLEAIDRKGLKQWT

Sequences:

>Translated_745_residues
MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEGVQAVVTHKDVPGLNRFGIVI
PDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEYKELEVMDSPEKALRPNAQRLHEDGNILHRAFFSNGDVEEG
FQASDTVFEETYELPRQMHTYMETEGGVAVPEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE
LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVADTGAYATLGPAVLDFSVEHA
AGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALETHLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGA
AQVLNAISKSPILKKTSRNCGYLQRGTGAAITMHGGGLGFGRMDAAGGRLSLSSEGKITASFGFEECGQGILAAIEQIVM
EELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGCSAENLIPGAAGLRDKNTKAL
VVTYKELAEKGPLAEETAFDFPTTPDPVVGGHFLYSFGAAAVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGG
AAMALGYTLMEEAKMTDGRYAAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGC
WINKLPISREELLEAIDRKGLKQWT
>Mature_745_residues
MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEGVQAVVTHKDVPGLNRFGIVI
PDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEYKELEVMDSPEKALRPNAQRLHEDGNILHRAFFSNGDVEEG
FQASDTVFEETYELPRQMHTYMETEGGVAVPEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE
LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVADTGAYATLGPAVLDFSVEHA
AGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALETHLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGA
AQVLNAISKSPILKKTSRNCGYLQRGTGAAITMHGGGLGFGRMDAAGGRLSLSSEGKITASFGFEECGQGILAAIEQIVM
EELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGCSAENLIPGAAGLRDKNTKAL
VVTYKELAEKGPLAEETAFDFPTTPDPVVGGHFLYSFGAAAVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGG
AAMALGYTLMEEAKMTDGRYAAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGC
WINKLPISREELLEAIDRKGLKQWT

Specific function: Oxidizes hypoxanthine and xanthine to uric acid

COG id: COG1529

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, large subunit CoxL/CutL homologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine dehydrogenase family

Homologues:

Organism=Homo sapiens, GI91823271, Length=718, Percent_Identity=27.0194986072423, Blast_Score=222, Evalue=8e-58,
Organism=Homo sapiens, GI71773480, Length=739, Percent_Identity=25.8457374830853, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1789230, Length=762, Percent_Identity=29.6587926509186, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1789246, Length=797, Percent_Identity=26.8506900878294, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786478, Length=773, Percent_Identity=25.8732212160414, Blast_Score=158, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17540638, Length=701, Percent_Identity=25.8202567760342, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17539860, Length=738, Percent_Identity=25.0677506775068, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566215, Length=758, Percent_Identity=24.0105540897098, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737937, Length=752, Percent_Identity=27.2606382978723, Blast_Score=213, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24647193, Length=690, Percent_Identity=24.2028985507246, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647199, Length=733, Percent_Identity=22.5102319236016, Blast_Score=117, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24647195, Length=727, Percent_Identity=23.3837689133425, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647197, Length=726, Percent_Identity=23.4159779614325, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647201, Length=685, Percent_Identity=22.3357664233577, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XDHD_BACSU (O32144)

Other databases:

- EMBL:   AL009126
- PIR:   B70017
- RefSeq:   NP_391128.1
- ProteinModelPortal:   O32144
- SMR:   O32144
- EnsemblBacteria:   EBBACT00000003711
- GeneID:   936686
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32480
- NMPDR:   fig|224308.1.peg.3254
- GenoList:   BSU32480
- GeneTree:   EBGT00050000001137
- HOGENOM:   HBG705921
- OMA:   RFQLARI
- PhylomeDB:   O32144
- ProtClustDB:   CLSK247866
- BioCyc:   BSUB:BSU32480-MONOMER
- BRENDA:   1.17.1.4
- InterPro:   IPR000674
- InterPro:   IPR008274
- InterPro:   IPR017609
- Gene3D:   G3DSA:3.30.365.10
- Gene3D:   G3DSA:3.90.1170.50
- TIGRFAMs:   TIGR03196

Pfam domain/function: PF01315 Ald_Xan_dh_C; PF02738 Ald_Xan_dh_C2; SSF56003 Ald_xan_DH_mo_bd; SSF54665 Aldxan_dh_hamm

EC number: =1.17.1.4

Molecular weight: Translated: 80435; Mature: 80435

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEG
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHEEEHHHHCC
VQAVVTHKDVPGLNRFGIVIPDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEY
CEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELEVMDSPEKALRPNAQRLHEDGNILHRAFFSNGDVEEGFQASDTVFEETYELPRQMHT
HHHHHCCCCHHHHCCCHHHHHCCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YMETEGGVAVPEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE
HHHCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEEEECCCCCCCCCCCC
LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVA
CCCCHHEEEEEECCCCCEEEEHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEE
DTGAYATLGPAVLDFSVEHAAGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALET
CCCCCHHHCHHHHEEEHHHCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEHHH
HLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGAAQVLNAISKSPILKKTSRNC
HHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCHHHCCCCC
GYLQRGTGAAITMHGGGLGFGRMDAAGGRLSLSSEGKITASFGFEECGQGILAAIEQIVM
CCEECCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHCCHHHHHHHHHHH
EELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGC
HHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAENLIPGAAGLRDKNTKALVVTYKELAEKGPLAEETAFDFPTTPDPVVGGHFLYSFGAA
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCE
AVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGGAAMALGYTLMEEAKMTDGRY
EEEEEEEEEECCEEEEECCHHHHCCCCCCCCCCCCEECCCEEHHHHHHHHHHHHCCCCCH
AAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGC
HHHCCCCEECCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
WINKLPISREELLEAIDRKGLKQWT
HHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEG
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHEEEHHHHCC
VQAVVTHKDVPGLNRFGIVIPDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEY
CEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELEVMDSPEKALRPNAQRLHEDGNILHRAFFSNGDVEEGFQASDTVFEETYELPRQMHT
HHHHHCCCCHHHHCCCHHHHHCCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YMETEGGVAVPEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE
HHHCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEEEECCCCCCCCCCCC
LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVA
CCCCHHEEEEEECCCCCEEEEHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEE
DTGAYATLGPAVLDFSVEHAAGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALET
CCCCCHHHCHHHHEEEHHHCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEHHH
HLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGAAQVLNAISKSPILKKTSRNC
HHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCHHHCCCCC
GYLQRGTGAAITMHGGGLGFGRMDAAGGRLSLSSEGKITASFGFEECGQGILAAIEQIVM
CCEECCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHCCHHHHHHHHHHH
EELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGC
HHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAENLIPGAAGLRDKNTKALVVTYKELAEKGPLAEETAFDFPTTPDPVVGGHFLYSFGAA
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCE
AVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGGAAMALGYTLMEEAKMTDGRY
EEEEEEEEEECCEEEEECCHHHHCCCCCCCCCCCCEECCCEEHHHHHHHHHHHHCCCCCH
AAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGC
HHHCCCCEECCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
WINKLPISREELLEAIDRKGLKQWT
HHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136