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The map label for this gene is pucE

Identifier: 16080300

GI number: 16080300

Start: 3335751

End: 3336272

Strand: Reverse

Name: pucE

Synonym: BSU32470

Alternate gene names: 16080300

Gene position: 3336272-3335751 (Counterclockwise)

Preceding gene: 16080301

Following gene: 16080294

Centisome position: 79.14

GC content: 52.3

Gene sequence:

>522_bases
ATGGACATAAAAGAGGCCGGGCCATTTCCTGTAAAAAAGGAACAGTTCCGGATGACCGTGAATGGTCAGGCGTGGGAGGT
TGCTGCCGTTCCTACGACACATCTGAGTGACCTGCTTAGAAAGGAATTTCAGCTGACCGGGACAAAGGTGTCCTGCGGAA
TCGGCCGCTGCGGAGCCTGCTCTATTTTAATCGACGGAAAACTGGCCAATGCGTGCATGACGATGGCCTATCAAGCAGAC
GGCCATTCCATCACAACCATCGAGGGGCTTCAAAAAGAAGAGCTGGATATGTGTCAAACCGCTTTCCTGGAAGAAGGCGG
CTTCCAATGCGGCTACTGTACGCCGGGAATGATCATTGCGCTTAAAGCATTGTTTCGGGAAACCCCTCAACCTTCTGACA
AAGATATAGAAGAAGGGCTGGCGGGGAATTTGTGCCGGTGTACCGGGTATGGCGGGATTATGCGGTCAGCTTGCAGGATT
AGAAGAGAGTTGAACGGGGGTAGACGGGAGTCCGGCTTTTAA

Upstream 100 bases:

>100_bases
TTGTAAAGGCAGTACATGATGCGGTCGGATGCTGGATAAACAAGCTGCCAATTTCAAGAGAAGAGTTGCTTGAAGCGATC
GACAGAAAGGGGCTGAAGCA

Downstream 100 bases:

>100_bases
AAGCCGGACTTTTTTAGCCTCAGTCAGACAGCGCGATCAAGACGCAGAAGCGTTGTGGGAAAACACACGATAAATCTCGT
AGAACTTGACAGCTTCTGGA

Product: xanthine dehydrogenase, iron-sulfur subunit

Products: NA

Alternate protein names: XDHase subunit E

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MDIKEAGPFPVKKEQFRMTVNGQAWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGACSILIDGKLANACMTMAYQAD
GHSITTIEGLQKEELDMCQTAFLEEGGFQCGYCTPGMIIALKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRI
RRELNGGRRESGF

Sequences:

>Translated_173_residues
MDIKEAGPFPVKKEQFRMTVNGQAWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGACSILIDGKLANACMTMAYQAD
GHSITTIEGLQKEELDMCQTAFLEEGGFQCGYCTPGMIIALKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRI
RRELNGGRRESGF
>Mature_173_residues
MDIKEAGPFPVKKEQFRMTVNGQAWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGACSILIDGKLANACMTMAYQAD
GHSITTIEGLQKEELDMCQTAFLEEGGFQCGYCTPGMIIALKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRI
RRELNGGRRESGF

Specific function: Oxidizes hypoxanthine and xanthine to uric acid

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain

Homologues:

Organism=Homo sapiens, GI91823271, Length=149, Percent_Identity=38.255033557047, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI71773480, Length=161, Percent_Identity=33.5403726708075, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786480, Length=168, Percent_Identity=36.9047619047619, Blast_Score=112, Evalue=9e-27,
Organism=Escherichia coli, GI1789232, Length=143, Percent_Identity=43.3566433566434, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540638, Length=149, Percent_Identity=41.6107382550336, Blast_Score=109, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17539860, Length=149, Percent_Identity=31.5436241610738, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566215, Length=161, Percent_Identity=29.1925465838509, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17737937, Length=158, Percent_Identity=37.3417721518987, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647197, Length=154, Percent_Identity=35.7142857142857, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647195, Length=154, Percent_Identity=35.7142857142857, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647199, Length=148, Percent_Identity=32.4324324324324, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647193, Length=154, Percent_Identity=32.4675324675325, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647201, Length=147, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XDHE_BACSU (O32143)

Other databases:

- EMBL:   AL009126
- PIR:   A70017
- RefSeq:   NP_391127.1
- ProteinModelPortal:   O32143
- SMR:   O32143
- EnsemblBacteria:   EBBACT00000000394
- GeneID:   936680
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32470
- NMPDR:   fig|224308.1.peg.3253
- GenoList:   BSU32470
- GeneTree:   EBGT00050000001959
- HOGENOM:   HBG292380
- OMA:   FLHERRD
- PhylomeDB:   O32143
- ProtClustDB:   CLSK2752501
- BioCyc:   BSUB:BSU32470-MONOMER
- BRENDA:   1.17.1.4
- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR017611
- Gene3D:   G3DSA:1.10.150.120
- Gene3D:   G3DSA:3.10.20.30
- TIGRFAMs:   TIGR03198

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2; SSF47741 2Fe-2S_bind; SSF54292 Ferredoxin

EC number: =1.17.1.4

Molecular weight: Translated: 18838; Mature: 18838

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEAGPFPVKKEQFRMTVNGQAWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGAC
CCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCEE
SILIDGKLANACMTMAYQADGHSITTIEGLQKEELDMCQTAFLEEGGFQCGYCTPGMIIA
EEEECCHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRIRRELNGGRRESGF
HHHHHHCCCCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDIKEAGPFPVKKEQFRMTVNGQAWEVAAVPTTHLSDLLRKEFQLTGTKVSCGIGRCGAC
CCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCEE
SILIDGKLANACMTMAYQADGHSITTIEGLQKEELDMCQTAFLEEGGFQCGYCTPGMIIA
EEEECCHHHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LKALFRETPQPSDKDIEEGLAGNLCRCTGYGGIMRSACRIRRELNGGRRESGF
HHHHHHCCCCCCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136