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The map label for this gene is thrC

Identifier: 16080278

GI number: 16080278

Start: 3313770

End: 3314828

Strand: Reverse

Name: thrC

Synonym: BSU32250

Alternate gene names: 16080278

Gene position: 3314828-3313770 (Counterclockwise)

Preceding gene: 16080279

Following gene: 16080277

Centisome position: 78.63

GC content: 48.25

Gene sequence:

>1059_bases
ATGTGGAAAGGACTTATCCATCAATATAAAGAATTTTTACCTGTAACAGATCAAACACCGGCGCTAACTTTACATGAAGG
AAACACACCTCTTATTCACCTGCCGAAGCTGTCTGAGCAGCTCGGAATTGAGCTTCATGTCAAAACGGAAGGCGTCAATC
CTACGGGATCATTTAAAGATCGCGGAATGGTTATGGCTGTGGCAAAGGCAAAAGAAGAAGGCAATGACACGATTATGTGC
GCGTCAACAGGTAACACTTCCGCTGCTGCGGCAGCATATGCAGCCCGTGCTAACATGAAATGCATTGTCATCATCCCGAA
CGGAAAAATTGCATTTGGAAAACTCGCTCAAGCTGTCATGTACGGAGCCGAGATTATCGCAATTGACGGAAACTTTGACG
ATGCGCTTAAAATTGTCCGTTCCATCTGTGAGAAATCACCGATTGCCCTTGTCAACTCAGTCAACCCTTACCGCATTGAA
GGCCAAAAAACTGCTGCCTTCGAAGTGTGCGAACAGCTGGGGGAAGCGCCTGATGTTTTAGCGATCCCGGTCGGAAATGC
TGGAAACATCACTGCGTACTGGAAGGGCTTCAAGGAATATCATGAGAAAAACGGCACAGGCCTTCCGAAAATGCGCGGCT
TTGAAGCTGAGGGTGCGGCGGCAATCGTGCGCAATGAAGTGATTGAAAATCCGGAAACAATAGCGACAGCCATTCGTATC
GGAAACCCGGCAAGCTGGGACAAAGCTGTAAAGGCAGCCGAGGAATCCAATGGGAAAATTGACGAAGTCACTGATGATGA
AATCCTTCACGCATATCAGCTGATCGCCCGTGTAGAAGGCGTGTTTGCAGAACCAGGTTCTTGCGCGTCTATCGCAGGAG
TGCTGAAACAGGTGAAATCCGGAGAAATTCCGAAAGGCAGCAAGGTCGTAGCTGTGTTAACAGGAAACGGACTGAAAGAT
CCGAACACAGCGGTCGACATTTCAGAAATCAAGCCTGTCACATTGCCGACTGATGAAGACAGCATCCTTGAATATGTAAA
AGGAGCGGCCCGTGTATGA

Upstream 100 bases:

>100_bases
CAGAAGCTGATTTCAGTGATATCCTGCAAAACCTAAATGATTTGGAAGTCGTTCAAGAAGTCAAAAGCACATATCGTGTA
GAAGGGAACGGTTGGAGCTA

Downstream 100 bases:

>100_bases
ACGAAGCCGACATGCTGTTCTCTGTCACTGTTCCCGGAAGCACAGCTAACCTAGGCCCCGGCTTTGATTCAGTCGGAATG
GCGCTCAGCAGATATTTGAA

Product: threonine synthase

Products: NA

Alternate protein names: TS

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMC
ASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIE
GQKTAAFEVCEQLGEAPDVLAIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAIVRNEVIENPETIATAIRI
GNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLTGNGLKD
PNTAVDISEIKPVTLPTDEDSILEYVKGAARV

Sequences:

>Translated_352_residues
MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMC
ASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIE
GQKTAAFEVCEQLGEAPDVLAIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAIVRNEVIENPETIATAIRI
GNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLTGNGLKD
PNTAVDISEIKPVTLPTDEDSILEYVKGAARV
>Mature_352_residues
MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMC
ASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIE
GQKTAAFEVCEQLGEAPDVLAIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAIVRNEVIENPETIATAIRI
GNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLTGNGLKD
PNTAVDISEIKPVTLPTDEDSILEYVKGAARV

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family

Homologues:

Organism=Escherichia coli, GI2367138, Length=210, Percent_Identity=27.6190476190476, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786185, Length=275, Percent_Identity=26.1818181818182, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790207, Length=337, Percent_Identity=25.2225519287834, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322631, Length=326, Percent_Identity=27.3006134969325, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THRC_BACSU (P04990)

Other databases:

- EMBL:   X04603
- EMBL:   AL009126
- EMBL:   M23217
- PIR:   A25364
- RefSeq:   NP_391105.1
- ProteinModelPortal:   P04990
- SMR:   P04990
- EnsemblBacteria:   EBBACT00000002083
- GeneID:   936660
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32250
- NMPDR:   fig|224308.1.peg.3231
- GenoList:   BSU32250
- GeneTree:   EBGT00070000031851
- HOGENOM:   HBG694883
- OMA:   VSVKADF
- ProtClustDB:   PRK07409
- BioCyc:   BSUB:BSU32250-MONOMER
- BRENDA:   4.2.3.1
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450
- TIGRFAMs:   TIGR00260

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.3.1

Molecular weight: Translated: 37464; Mature: 37464

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: BINDING 85-85 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKD
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCHHHCCCEEEEEECCCCCCCCCCC
RGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVM
CCEEEEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
YGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQKTAAFEVCEQLGEAPDVL
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCEE
AIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAIVRNEVIENPETIATAIRI
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEEEC
GNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKS
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GEIPKGSKVVAVLTGNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV
CCCCCCCEEEEEEECCCCCCCCCEEEHHHCCCEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKD
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCHHHCCCEEEEEECCCCCCCCCCC
RGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVM
CCEEEEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
YGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQKTAAFEVCEQLGEAPDVL
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCCCCEE
AIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAIVRNEVIENPETIATAIRI
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEEEEEC
GNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKS
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GEIPKGSKVVAVLTGNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV
CCCCCCCEEEEEEECCCCCCCCCEEEHHHCCCEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3098560; 9384377; 3139660