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The map label for this gene is thrB

Identifier: 16080277

GI number: 16080277

Start: 3312844

End: 3313773

Strand: Reverse

Name: thrB

Synonym: BSU32240

Alternate gene names: 16080277

Gene position: 3313773-3312844 (Counterclockwise)

Preceding gene: 16080278

Following gene: 255767740

Centisome position: 78.61

GC content: 51.08

Gene sequence:

>930_bases
ATGAACGAAGCCGACATGCTGTTCTCTGTCACTGTTCCCGGAAGCACAGCTAACCTAGGCCCCGGCTTTGATTCAGTCGG
AATGGCGCTCAGCAGATATTTGAAGCTGACCGTCTTTGAAAGCGACAAATGGTCTTTTGAGGCTGAAACAGAAACAGTCG
CCGGAATTCCGGCGGGTACAGATAACCTGATCTACCAAGTGGCTAAACGGACCGCAGATTTGTACGGAAAAGAAATGCCT
CCTGTCCATGTGAAGGTGTGGAGCGACATCCCGCTTGCACGCGGCCTTGGCAGCAGCGCCGCAGCGATTGTAGCGGCCAT
TGAACTGGCTGATGAATTATGCGGCTTAAAGCTGTCTGAAGCGGACAAGCTGCATTTAGCGAGTCTAGAAGAAGGACACC
CGGACAATGCTGGCGCTTCTCTCGTCGGCGGACTTGTGATCGGCCTGCATGAGGATGACGAGACCCAAATGATCCGCGTC
CCGAATGCTGACATTGACGTAGTCGTTGTCATTCCTTTTTATGAAGTGCTGACAAGAGACGCGAGAGACGTGCTTCCGAA
GGAGTTTCCATATGCCGATGCCGTAAAAGCAAGTGCTGTCAGCAATATCCTCATTGCTGCGATCATGTCCAAGGATTGGC
CGCTTGTCGGGAAAATCATGAAGAAGGATATGTTCCATCAGCCGTACCGGGCGATGCTTGTGCCTGAGCTGTCAAAAGTA
GAGCACGTCGCCGAGATGAAGGGCGCATATGGAACGGCTCTCAGCGGAGCAGGCCCAACGATTCTCGTCATGACCGAAAA
AGGAAAGGGAGAAGAGCTAAAAGAACAGCTCGCGCTTCATTTCCCTCATTGTGAAGTAGACGCTTTGACCGTTCCGAAAG
AGGGAAGTATAATAGAGCGAAATCCTTTATATCAAGTAAAAAGTGTATAG

Upstream 100 bases:

>100_bases
AAGATCCGAACACAGCGGTCGACATTTCAGAAATCAAGCCTGTCACATTGCCGACTGATGAAGACAGCATCCTTGAATAT
GTAAAAGGAGCGGCCCGTGT

Downstream 100 bases:

>100_bases
ATCATGCCAAGCGTTCCTCAGCGTCAGCGGAGGGCGCTTTTTTTTGCTGGAGATAAAGGAGTTTTTATTTAGAAAAACGA
AATACATGTAAAAAGGAGGA

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGTDNLIYQVAKRTADLYGKEMP
PVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRV
PNADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKV
EHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV

Sequences:

>Translated_309_residues
MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGTDNLIYQVAKRTADLYGKEMP
PVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRV
PNADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKV
EHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV
>Mature_309_residues
MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGTDNLIYQVAKRTADLYGKEMP
PVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRV
PNADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKV
EHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=275, Percent_Identity=29.0909090909091, Blast_Score=108, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321814, Length=331, Percent_Identity=30.5135951661631, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_BACSU (P04948)

Other databases:

- EMBL:   X04603
- EMBL:   AL009126
- PIR:   H69722
- RefSeq:   NP_391104.1
- ProteinModelPortal:   P04948
- SMR:   P04948
- EnsemblBacteria:   EBBACT00000000953
- GeneID:   936665
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32240
- NMPDR:   fig|224308.1.peg.3230
- GenoList:   BSU32240
- GeneTree:   EBGT00050000000874
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- PhylomeDB:   P04948
- ProtClustDB:   PRK01212
- BioCyc:   BSUB:BSU32240-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 33325; Mature: 33325

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGT
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCEECCCCEEECCCCCC
DNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSE
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
ADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRVPNADIDVVVVIPFYEVLTRD
CCCEEEECCCCCCCCCCCHHEECEEEEEEECCCCCEEEECCCCCEEEEEEECHHHHHHHH
ARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHH
EHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIER
HHHHHHCCCCCCEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEC
NPLYQVKSV
CCCEEECCC
>Mature Secondary Structure
MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGT
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCEECCCCEEECCCCCC
DNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSE
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
ADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRVPNADIDVVVVIPFYEVLTRD
CCCEEEECCCCCCCCCCCHHEECEEEEEEECCCCCEEEECCCCCEEEEEEECHHHHHHHH
ARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHH
EHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIER
HHHHHHCCCCCCEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEC
NPLYQVKSV
CCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3098560; 9384377