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The map label for this gene is yuiE

Identifier: 16080258

GI number: 16080258

Start: 3296417

End: 3297919

Strand: Reverse

Name: yuiE

Synonym: BSU32050

Alternate gene names: 16080258

Gene position: 3297919-3296417 (Counterclockwise)

Preceding gene: 16080260

Following gene: 16080257

Centisome position: 78.23

GC content: 47.64

Gene sequence:

>1503_bases
ATGTTTTATGCGGTTCAACAATCAGAGCATACAGAAACCCTTGTTGTCGGGCTTTTTCAAAAAAGCCAGCTGACAGGAAA
AGCGTTGGAAATTGATGAAATGCTCGAAGGCCATCTGACACAGCTTTTAAAAGAGGGTGATGTCTCTGCGAAACCGAATC
AAGTATCAAAAGTATTCCCTCCATCTTCTGCGGGTATGAAACGGATTTATTTCGTTGGCCTGGGGAGAGAAGCAAATTAT
TCGTTTGAACAGGCGAAGGAACGTTTTGCTCATGTTTTTCAAGCCATTCATAAAGATCGAAAACAAGAAACTGCTGTTCT
GCTTGATACGTTTATTTCGGAAGACGTGCCTCCAGCCGATGCCGCTCATGCTTTAGCTGAGAGCTGCCTGCTTGCTTCCT
ATGAGGTGCAGGATTATAAGCATAAATCAAATGAGCCTGACAAACAAATTGAAGCCGTGTATGTTGTAACAGATGAAGAT
ACACAGGAAGTGCAGGCAGGGCTTCGCGTCGGCCAGGCATACGGACAAGGGACAAATTCGGCTAGAACGCTTGTGAATAT
GCCGGGAAACATGCTGACAGCGACTGACCTCGCTTCGTATGCTGAAGAGCTTGCGGCGAAATATGATTTTGAGTGTGAGA
TATTGGAGAAATCAGAGATGGAAGAGCTCGGTATGGGCGGAATACTTGCGGTAAACCAAGGCTCCACCGAACCGCCGAAA
ATGATTGTGTTGAAATATCAGGGCAAAAAGGAATGGGAAGACGTCGTCGGACTAGTCGGGAAAGGAATTACCTTTGACAC
AGGCGGCTACTCCATTAAAACGAAATCTGGAATTGTTGGCATGAAATCAGATATGGGAGGAGCAGCTGCGGTTCTCGGGG
CTATGGAAACGATCGGAGAGCTTCGTCCTGAACAAAACGTGCTGTGCGTTATTCCATCAACCGATAATATGATCTCCGGC
GGGGCCATGAAGCCGGATGACGTGATCGTATCTTTAAGCGGAAAAACAATAGAAATCCTTAATACGGATGCTGAAGGAAG
GCTAGTGCTTGCAGATGGCATTACATACGCGAAACAGCATGGCGCATCAGTTCTTGTCGATGTAGCTACGCTTACCGGCG
GCGTCGTTGTGGCATTAGGAACAGAAACAACCGGTGCAATGACAAATGATCAAAGCTTTTACCAGCAGGTAGCTGATGCT
GCACAAGAGTGCGGCGAGGCGATCTGGCAGCTGCCGATAACAGAGAAGGATAAAAAACGCGTGAAAAGCAGCCAAATGGC
GGATCTCAGCAATTCACCGGGACGCGAGGGACATGCGATTATGGCAGGAACGTTTCTTGGTGAATTCGCTGAGTCAACAC
CTTGGGTTCACCTTGATATTGCAGGCACAGCGACTGCCAATAAAGCAACCTGCTTCGGACCGGCCGGGGCTACTGGCGTT
ATGGCAAGAACACTGGCTACACTTGCGGAGCGATTTACATTGGAAGAAGATAAAAACGAGTAA

Upstream 100 bases:

>100_bases
TTAGACTGTTGTTTGTTAAAATAAGTATAGTGGAAAGAATGTTTTTCCTATTATACCATTGTTCATTACAAATACCCATT
ACGTTAGGAGTGTAAGCCTC

Downstream 100 bases:

>100_bases
TGAAAAATCCCTGCTGTATGATGCGGCAGGGATTTTTTCTTCCGAAATCTTTAGTTGACTAAAGTGGATCGGTTATGATA
CGATACGTTCGTACTTTATT

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MFYAVQQSEHTETLVVGLFQKSQLTGKALEIDEMLEGHLTQLLKEGDVSAKPNQVSKVFPPSSAGMKRIYFVGLGREANY
SFEQAKERFAHVFQAIHKDRKQETAVLLDTFISEDVPPADAAHALAESCLLASYEVQDYKHKSNEPDKQIEAVYVVTDED
TQEVQAGLRVGQAYGQGTNSARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEELGMGGILAVNQGSTEPPK
MIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKSDMGGAAAVLGAMETIGELRPEQNVLCVIPSTDNMISG
GAMKPDDVIVSLSGKTIEILNTDAEGRLVLADGITYAKQHGASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADA
AQECGEAIWQLPITEKDKKRVKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGTATANKATCFGPAGATGV
MARTLATLAERFTLEEDKNE

Sequences:

>Translated_500_residues
MFYAVQQSEHTETLVVGLFQKSQLTGKALEIDEMLEGHLTQLLKEGDVSAKPNQVSKVFPPSSAGMKRIYFVGLGREANY
SFEQAKERFAHVFQAIHKDRKQETAVLLDTFISEDVPPADAAHALAESCLLASYEVQDYKHKSNEPDKQIEAVYVVTDED
TQEVQAGLRVGQAYGQGTNSARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEELGMGGILAVNQGSTEPPK
MIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKSDMGGAAAVLGAMETIGELRPEQNVLCVIPSTDNMISG
GAMKPDDVIVSLSGKTIEILNTDAEGRLVLADGITYAKQHGASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADA
AQECGEAIWQLPITEKDKKRVKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGTATANKATCFGPAGATGV
MARTLATLAERFTLEEDKNE
>Mature_500_residues
MFYAVQQSEHTETLVVGLFQKSQLTGKALEIDEMLEGHLTQLLKEGDVSAKPNQVSKVFPPSSAGMKRIYFVGLGREANY
SFEQAKERFAHVFQAIHKDRKQETAVLLDTFISEDVPPADAAHALAESCLLASYEVQDYKHKSNEPDKQIEAVYVVTDED
TQEVQAGLRVGQAYGQGTNSARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEELGMGGILAVNQGSTEPPK
MIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKSDMGGAAAVLGAMETIGELRPEQNVLCVIPSTDNMISG
GAMKPDDVIVSLSGKTIEILNTDAEGRLVLADGITYAKQHGASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADA
AQECGEAIWQLPITEKDKKRVKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGTATANKATCFGPAGATGV
MARTLATLAERFTLEEDKNE

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family

Homologues:

Organism=Homo sapiens, GI41393561, Length=516, Percent_Identity=33.5271317829457, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI47155554, Length=371, Percent_Identity=32.3450134770889, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1790710, Length=491, Percent_Identity=34.6232179226069, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082123, Length=317, Percent_Identity=37.2239747634069, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556903, Length=303, Percent_Identity=37.6237623762376, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17565172, Length=149, Percent_Identity=40.9395973154362, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24661038, Length=326, Percent_Identity=32.2085889570552, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI21355725, Length=334, Percent_Identity=31.7365269461078, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI20129969, Length=468, Percent_Identity=28.8461538461538, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24662227, Length=494, Percent_Identity=26.9230769230769, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21357381, Length=284, Percent_Identity=34.8591549295775, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI221379063, Length=284, Percent_Identity=34.8591549295775, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI221379062, Length=284, Percent_Identity=34.8591549295775, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI161077148, Length=458, Percent_Identity=27.5109170305677, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI20130057, Length=458, Percent_Identity=27.5109170305677, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI21355645, Length=461, Percent_Identity=26.6811279826464, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24662223, Length=461, Percent_Identity=26.6811279826464, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19922386, Length=471, Percent_Identity=26.7515923566879, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI20129963, Length=461, Percent_Identity=26.8980477223427, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24646701, Length=257, Percent_Identity=28.0155642023346, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24646703, Length=257, Percent_Identity=28.0155642023346, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21358201, Length=257, Percent_Identity=28.0155642023346, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPA_BACSU (O32106)

Other databases:

- EMBL:   AL009126
- PIR:   F70012
- RefSeq:   NP_391085.1
- ProteinModelPortal:   O32106
- SMR:   O32106
- MEROPS:   M17.010
- EnsemblBacteria:   EBBACT00000001369
- GeneID:   936587
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32050
- NMPDR:   fig|224308.1.peg.3211
- GenoList:   BSU32050
- GeneTree:   EBGT00050000002613
- HOGENOM:   HBG742580
- OMA:   VYACKLK
- PhylomeDB:   O32106
- ProtClustDB:   PRK00913
- BioCyc:   BSUB:BSU32050-MONOMER
- BRENDA:   3.4.11.1
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00181
- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283
- PANTHER:   PTHR11963:SF3
- PRINTS:   PR00481

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N

EC number: =3.4.11.1; =3.4.11.10

Molecular weight: Translated: 53658; Mature: 53658

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00631 CYTOSOL_AP

Important sites: ACT_SITE 273-273 ACT_SITE 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYAVQQSEHTETLVVGLFQKSQLTGKALEIDEMLEGHLTQLLKEGDVSAKPNQVSKVFP
CEEEECCCCCCCEEEEEEHHHCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PSSAGMKRIYFVGLGREANYSFEQAKERFAHVFQAIHKDRKQETAVLLDTFISEDVPPAD
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AAHALAESCLLASYEVQDYKHKSNEPDKQIEAVYVVTDEDTQEVQAGLRVGQAYGQGTNS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCC
ARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEELGMGGILAVNQGSTEPPK
CCEEEECCCCEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCEEEECCCCCCCCE
MIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKSDMGGAAAVLGAMETIGE
EEEEEECCCCHHHHHHHHHCCCCEECCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHH
LRPEQNVLCVIPSTDNMISGGAMKPDDVIVSLSGKTIEILNTDAEGRLVLADGITYAKQH
CCCCCCEEEEECCCCCEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHHHHC
GASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADAAQECGEAIWQLPITEKDKKR
CCEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGTATANKATCFGPAGATGV
HHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCHHH
MARTLATLAERFTLEEDKNE
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFYAVQQSEHTETLVVGLFQKSQLTGKALEIDEMLEGHLTQLLKEGDVSAKPNQVSKVFP
CEEEECCCCCCCEEEEEEHHHCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PSSAGMKRIYFVGLGREANYSFEQAKERFAHVFQAIHKDRKQETAVLLDTFISEDVPPAD
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AAHALAESCLLASYEVQDYKHKSNEPDKQIEAVYVVTDEDTQEVQAGLRVGQAYGQGTNS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCC
ARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEELGMGGILAVNQGSTEPPK
CCEEEECCCCEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCEEEECCCCCCCCE
MIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKSDMGGAAAVLGAMETIGE
EEEEEECCCCHHHHHHHHHCCCCEECCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHH
LRPEQNVLCVIPSTDNMISGGAMKPDDVIVSLSGKTIEILNTDAEGRLVLADGITYAKQH
CCCCCCEEEEECCCCCEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEECCCHHHHHC
GASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADAAQECGEAIWQLPITEKDKKR
CCEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGTATANKATCFGPAGATGV
HHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCHHH
MARTLATLAERFTLEEDKNE
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377