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The map label for this gene is menF

Identifier: 16080135

GI number: 16080135

Start: 3152302

End: 3153717

Strand: Reverse

Name: menF

Synonym: BSU30830

Alternate gene names: 16080135

Gene position: 3153717-3152302 (Counterclockwise)

Preceding gene: 16080137

Following gene: 16080134

Centisome position: 74.81

GC content: 46.33

Gene sequence:

>1416_bases
ATGGTGACAACGGTGCAGCGTACGTTCCGAAAGGAAGTTCTACATGCATTACATAAAGCCAAAGAAGTCAACCATGCTGT
CTTAATAAGCTATTCGAGACAAATCGAGTCTCTTGACCCTCTATCATTTTTCAATTACGGAGCAAAAAAATATACAGGCA
ATCGATTTTTTTGGTCAGATCCTGAAAGTGAATTGACAATAGTCGGTCTTGGCAAAGAAGCGGTTTTCCAGACAAATCAA
AAAAACAGCGAGCGGTATCGTGAGGTTTTTGAACAATGGGAGCGCTTTAAAAAGACGGCTTTTCATATTTATGAAGAAGA
AAAGCTGCAGCATTCTGCAGTGGGACCTGTGTTATTCGGAGGATTTTCTTTTGACCCTTGCGAAGAAAGAGGTTCACAAT
GGGACCATTTCTCGGAAGGGGATTTCTTTGTGCCTGCGCTTATGCTGACGATGACTGCTGAAGGCCCGTTCTTAACAGTT
AACAGATGGGTAAGCGGAGGAGAAGACGCAGAAGCTGTTTTAGAAGGCTTAAAAGCTTTTGCGGCGGAATTTATGGTTCC
CGATTTCAAGCAAGAAGATCAGGCTGTGATTGCAGCAGCCGAAGAGCTGGATAAGGATGATTGGCTGAAAGCAATCGAAA
CGGCCACAAGCCAAATTAAAGAGAAACAATATGATAAGGTTGTTCTTGCCCGAGAGCTGCTGCTCACGTTTGACGGTCCA
ATCCAAATTGAACCGGTGCTTAAAACGCTTCTGGACGATCAGCAGACAAGCTATGTTTTTGCAATTGAACAAGAAGGCAA
AACCTTTGTCGGCGCGTCTCCGGAAAGACTGATCAAAAGAGACGGCGGCACTGTCATGTCTTCCTGTCTGGCAGGCTCCA
TTAAACGAGGCGTGAATGAAGAAGACGACCGCCGGATAGGCCTTGAATTATTAAACGATGAGAAAAACCTGCTTGAGCAT
GATATTGTGGTAGGTATGATTCATAATGCTTTTGTATCAAGCTGTTCAGAGGTTGAAAAGCCTGATGGACCTGTTTTGTA
CAAAACAAAAAGCGTTCAGCACCTGTTTACGCCGATTGTCGGACAGCTGCGCGAGTCTGCTTCGATTTTTGACTTAATTG
AGAAGCTGCACCCCACTCCCGCGCTTGGGGGATCACCTCAGGAAAAAGCTGTTGACGTGATCAGGGAGATTGAACCGATG
TCCCGCGGCTGGTATGCGGCCCCTATCGGCTGGATTGACAGCCAGGATAACGGGGAATTCGCGGTCGCCATTCGTTCAGG
GCTCATCGAAGGAAGCACAGCAAGGCTGTTTGCCGGGTGCGGTATTGTGGAAGACTCGGAACCAATCTCGGAATATGAAG
AAACACAGATTAAATTGAAGCCGATGATCTCTGCATTAGGAGGTGAGAGGCGTTGA

Upstream 100 bases:

>100_bases
GGTAAGGCAGGCCATTTGGACTGCATGATCTGTGTTTGACACAAAGGAGACACAGGTGTATGGTATTAAAAGCATTTTAA
GGATTATGGAGGAATGTCTC

Downstream 100 bases:

>100_bases
CAGTCAACCCGATTACTCATTACATCGGAAGTTTTATTGATGAATTCGCCCTTTCCGGAATTACGGACGCCGTCGTTTGT
CCGGGCTCAAGATCTACACC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSDPESELTIVGLGKEAVFQTNQ
KNSERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFGGFSFDPCEERGSQWDHFSEGDFFVPALMLTMTAEGPFLTV
NRWVSGGEDAEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGP
IQIEPVLKTLLDDQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEH
DIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPM
SRGWYAAPIGWIDSQDNGEFAVAIRSGLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR

Sequences:

>Translated_471_residues
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSDPESELTIVGLGKEAVFQTNQ
KNSERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFGGFSFDPCEERGSQWDHFSEGDFFVPALMLTMTAEGPFLTV
NRWVSGGEDAEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGP
IQIEPVLKTLLDDQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEH
DIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPM
SRGWYAAPIGWIDSQDNGEFAVAIRSGLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR
>Mature_471_residues
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSDPESELTIVGLGKEAVFQTNQ
KNSERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFGGFSFDPCEERGSQWDHFSEGDFFVPALMLTMTAEGPFLTV
NRWVSGGEDAEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGP
IQIEPVLKTLLDDQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEH
DIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPM
SRGWYAAPIGWIDSQDNGEFAVAIRSGLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family

Homologues:

Organism=Escherichia coli, GI1786809, Length=259, Percent_Identity=37.4517374517375, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082077, Length=256, Percent_Identity=32.8125, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787518, Length=259, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788114, Length=204, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=226, Percent_Identity=30.5309734513274, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENF_BACSU (P23973)

Other databases:

- EMBL:   M74521
- EMBL:   M74521
- EMBL:   M74538
- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   M21320
- PIR:   A69657
- RefSeq:   NP_390961.1
- ProteinModelPortal:   P23973
- SMR:   P23973
- EnsemblBacteria:   EBBACT00000003820
- GeneID:   937190
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30830
- NMPDR:   fig|224308.1.peg.3086
- GenoList:   BSU30830
- GeneTree:   EBGT00050000001425
- HOGENOM:   HBG405459
- OMA:   KAFVQRE
- PhylomeDB:   P23973
- ProtClustDB:   CLSK887766
- BioCyc:   BSUB:BSU30830-MONOMER
- BioCyc:   MetaCyc:MONOMER-13806
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236:SF3
- PANTHER:   PTHR11236
- TIGRFAMs:   TIGR00543

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 52812; Mature: 52812

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSD
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCHHHHHCCCHHCCCCEEEECC
PESELTIVGLGKEAVFQTNQKNSERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFG
CCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GFSFDPCEERGSQWDHFSEGDFFVPALMLTMTAEGPFLTVNRWVSGGEDAEAVLEGLKAF
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGP
HHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IQIEPVLKTLLDDQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNE
EEEHHHHHHHHCCCCCCEEEEEECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
EDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIV
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
GQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRGWYAAPIGWIDSQDNGEF
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCE
AVAIRSGLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR
EEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSD
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCHHHHHCCCHHCCCCEEEECC
PESELTIVGLGKEAVFQTNQKNSERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFG
CCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GFSFDPCEERGSQWDHFSEGDFFVPALMLTMTAEGPFLTVNRWVSGGEDAEAVLEGLKAF
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
AAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGP
HHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IQIEPVLKTLLDDQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNE
EEEHHHHHHHHCCCCCCEEEEEECCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
EDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIV
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
GQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRGWYAAPIGWIDSQDNGEF
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCE
AVAIRSGLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR
EEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310