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The map label for this gene is ytpA

Identifier: 16080103

GI number: 16080103

Start: 3122662

End: 3123441

Strand: Reverse

Name: ytpA

Synonym: BSU30510

Alternate gene names: 16080103

Gene position: 3123441-3122662 (Counterclockwise)

Preceding gene: 16080105

Following gene: 16080102

Centisome position: 74.09

GC content: 43.97

Gene sequence:

>780_bases
ATGTGGACCTGGAAAGCAGACAGACCTGTTGCCGTGATTGTAATCATTCACGGGGCAAGTGAATATCACGGACGATATAA
ATGGCTGATTGAAATGTGGCGGTCTTCGGGTTATCACGTTGTCATGGGCGATTTGCCTGGACAGGGAACAACGACAAGAG
CGAGAGGGCATATTCGCTCGTTTCAAGAATACATTGATGAAGTAGATGCTTGGATAGATAAAGCGCGAACCTTTGATCTT
CCGGTTTTTCTTCTAGGTCACAGCATGGGCGGTCTGGTCGCGATTGAATGGGTTAAACAGCAAAGAAATCCCCGGATTAC
GGGAATTATTTTATCATCACCGTGTCTCGGACTGCAAATTAAAGTGAATAAAGCGCTTGATCTTGCATCAAAAGGCCTGA
ATGTCATCGCTCCGTCACTTAAGGTTGATTCGGGATTATCAATTGATATGGCGACGCGCAATGAGGACGTCATCGAAGCT
GACCAAAATGATTCGTTATACGTCAGGAAAGTTTCTGTCAGATGGTACAGAGAGCTTTTAAAGACAATTGAATCGGCAAT
GGTGCCGACAGAGGCATTTTTGAAAGTTCCGCTTCTTGTGATGCAAGCCGGTGATGACAAGCTTGTTGACAAAACGATGG
TCATCAAGTGGTTTAATGGTGTTGCTTCTCATAATAAAGCATACAGAGAGTGGGAAGGGCTTTACCATGAGATCTTCAAT
GAACCCGAGAGAGAAGATGTTTTTAAAGCAGCAAGAGCGTTTACTGATCAATATATTTGA

Upstream 100 bases:

>100_bases
TAAAGGCTGTTGGGTGGATATCTGGCTTGTGTTCCTTATAAGGGTAGATCATAAGATTCATCCTTCTTTCTCTATTTAAA
TTTCGTATGAGGTGAAGATC

Downstream 100 bases:

>100_bases
ACTAGTAGGGGGTGCATGTGTTGACAGTACCGGAACATCCTTTTGGATTGATGGCAAAGGTATATCGAGATATTTTTCCG
CTCGTTCATCAAGAGCTGGA

Product: phospholipase component of bacilysocin synthesis or export

Products: NA

Alternate protein names: Bacilysocin biosynthesis protein ytpA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRSFQEYIDEVDAWIDKARTFDL
PVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQIKVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEA
DQNDSLYVRKVSVRWYRELLKTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFN
EPEREDVFKAARAFTDQYI

Sequences:

>Translated_259_residues
MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRSFQEYIDEVDAWIDKARTFDL
PVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQIKVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEA
DQNDSLYVRKVSVRWYRELLKTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFN
EPEREDVFKAARAFTDQYI
>Mature_259_residues
MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRSFQEYIDEVDAWIDKARTFDL
PVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQIKVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEA
DQNDSLYVRKVSVRWYRELLKTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFN
EPEREDVFKAARAFTDQYI

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily

Homologues:

Organism=Homo sapiens, GI51242953, Length=248, Percent_Identity=29.8387096774194, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI6005786, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787678, Length=260, Percent_Identity=24.2307692307692, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLBAC_BACSU (O34705)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   D69998
- RefSeq:   NP_390929.1
- ProteinModelPortal:   O34705
- SMR:   O34705
- EnsemblBacteria:   EBBACT00000002820
- GeneID:   937237
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30510
- NMPDR:   fig|224308.1.peg.3054
- GenoList:   BSU30510
- GeneTree:   EBGT00050000001474
- HOGENOM:   HBG748003
- OMA:   IFNEPER
- PhylomeDB:   O34705
- ProtClustDB:   CLSK873569
- BioCyc:   BSUB:BSU30510-MONOMER
- InterPro:   IPR000073
- InterPro:   IPR022742
- PRINTS:   PR00111

Pfam domain/function: PF12146 Hydrolase_4

EC number: NA

Molecular weight: Translated: 29566; Mature: 29566

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00120 LIPASE_SER

Important sites: ACT_SITE 88-88 ACT_SITE 206-206 ACT_SITE 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRS
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
FQEYIDEVDAWIDKARTFDLPVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQI
HHHHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEE
KVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEADQNDSLYVRKVSVRWYRELL
EECHHHHHHHCCCCEEECCEEECCCCEEEEECCCCCCEECCCCCCEEEEEEHHHHHHHHH
KTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFN
HHHHHHCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EPEREDVFKAARAFTDQYI
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWTWKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRS
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
FQEYIDEVDAWIDKARTFDLPVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQI
HHHHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEE
KVNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEADQNDSLYVRKVSVRWYRELL
EECHHHHHHHCCCCEEECCEEECCCCEEEEECCCCCCEECCCCCCEEEEEEHHHHHHHHH
KTIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFN
HHHHHHCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EPEREDVFKAARAFTDQYI
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377