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The map label for this gene is malS

Identifier: 16080040

GI number: 16080040

Start: 3056849

End: 3058549

Strand: Reverse

Name: malS

Synonym: BSU29880

Alternate gene names: 16080040

Gene position: 3058549-3056849 (Counterclockwise)

Preceding gene: 16080041

Following gene: 16080038

Centisome position: 72.55

GC content: 47.85

Gene sequence:

>1701_bases
GTGAAACAATTCAGAGTAACAAACGAAGGAGACATTCAAACAACATTAAGAGGCCTTGAAGTGCTCTCTGTTCCTTTTTT
GAACAAAGGTGTTGCTTTTACTGAGGAAGAAAGAAAAGAACTTGGTTTAAAAGGCTTTCTGCCGCCCAAGGTATTAACAA
TTGATGATCAGGCGAAAAGAGCGTATGAACAATATTCCGCACAGCCTGACGATTTGAGCAAAAATGTCTATTTAACGGCG
CTGCACGATCGGAATGAAACGCTGTTTTACCGCCTGTTAAATGACCATTTAGGGGAAATGCTCCCGATTGTCTACACACC
GACAGTCGGAACGGCAATTCAGAGATACAGCCATGAATACAGAAAACCACGAGGGCTCTATCTGTCAATCGATGACCCGG
ACGGTATGAAAGAAGCTTTTAAACAGTATAAAGATCAAAGCGATACGATCGATTTAATTGTTGCAACTGATGCGGAAGGA
ATTTTAGGAATCGGTGACTGGGGCGTCGGAGGAATCGCAATTTCTATTGGCAAGCTGGCGGTTTATACAGCGGCTGCGGG
CATTGATCCGAGCAGAGTGCTGGCTGTTGTTTTAGATGCGGGGACAAATCAGGAGAGCCTCTTGAACGACCCGCTTTATG
TCGGCAACCAGCATTCCAGAGTGCGCGGGGAACGTTATGACCAATTTATTGACGACTACGTTGCGCTGGCGAGAGAAACA
TTTCCGAATGCATTGCTGCATTGGGAGGATTTCGGGGCGAAAAACGCGCGAAGCATTTTGAAACGCTATAAAGATAAAGT
GTGTACCTTCAATGATGATATCCAAGGAACTGGCGCTGTTTCTTTGGCCGCAGTCTTATCCTGTGCAAAAGCGTCTAAAG
TTCCGCTGAGAGATCACAGAGTTGTTATATTCGGAGCGGGAACGGCAGGAATCGGAATTGCCGAACAGCTGCGGGAAGCA
TTGGTGCGCGAGGGACTGAGTGAAGAAGAATCATACAAGCGCTTCTGGTGCATAGACAGGAACGGGCTGTTAACAGATGA
TATGGATCAGCTTCTTGATTTCCAAAAGCCGTATGCCCGTTCGGCTGATGAAGTGAAGGATTATCAGCGGAACGGAGACG
GCGGAGGCATTGATCTTCTTGAAGTGGTTCGGCAGGCGAAACCGACGATCCTGATCGGCACATCTACGGTTTCCGGCGCT
TTCACTGAAGAAATTGTAAAAGAAATGGCGTCACATGTGAAACGGCCGGCGATTTTGCCAATGTCAAATCCGACAACTCT
TTCTGAAGCAAAGCCGGAGGATTTAATCGAATGGACAGAAGGCCGTGCGCTGATCACGACAGGAAGCCCGTTCCCGCCAG
TTGAATATAACGGAGTTACGTATCATATCGGGCAGGCAAACAACGCGCTTGTCTTCCCTGGTCTCGGTCTTGGAACGATT
GTTACAAAATCAAAATTAATTACCGACGGCATGTTTGAAGCATGTGCCCGGGCAATCGCAGGTATGGTCAATGTCGGGGT
GCCTGGGGCGCCGATGCTTCCAAAAGTTGAAGATTTGCGCACTGTTTCGGCAACCGTTGCTGTTGAAGTTGCAAAAACCG
CAATGAAGGAAGGCGTCGCTACGGAAGAGCCTGAAGACATTATACAGGCAGTGCAGGATGCGATGTGGTATCCGGTATAC
AAACCGATTCGCGCGATATAA

Upstream 100 bases:

>100_bases
TAAGCGAATGAAGCGATAGCATATTTTTATGTTTACCCTGCATGTTTTCCGGCTAATAATGCAAACCTAGTACTATTATG
CTCAGAAAGGAAGAGGCAGG

Downstream 100 bases:

>100_bases
TAAAAAAGAGCCGCTGCATAGGCAGCGGCTTCTATTTTTTGCTTGTCCGGTGTTAAGAGGCGATTGTATCAATGACATCA
ACAATTTCTCCCGTTTTCGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKQFRVTNEGDIQTTLRGLEVLSVPFLNKGVAFTEEERKELGLKGFLPPKVLTIDDQAKRAYEQYSAQPDDLSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPDGMKEAFKQYKDQSDTIDLIVATDAEG
ILGIGDWGVGGIAISIGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARET
FPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHRVVIFGAGTAGIGIAEQLREA
LVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYARSADEVKDYQRNGDGGGIDLLEVVRQAKPTILIGTSTVSGA
FTEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIEWTEGRALITTGSPFPPVEYNGVTYHIGQANNALVFPGLGLGTI
VTKSKLITDGMFEACARAIAGMVNVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVATEEPEDIIQAVQDAMWYPVY
KPIRAI

Sequences:

>Translated_566_residues
MKQFRVTNEGDIQTTLRGLEVLSVPFLNKGVAFTEEERKELGLKGFLPPKVLTIDDQAKRAYEQYSAQPDDLSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPDGMKEAFKQYKDQSDTIDLIVATDAEG
ILGIGDWGVGGIAISIGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARET
FPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHRVVIFGAGTAGIGIAEQLREA
LVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYARSADEVKDYQRNGDGGGIDLLEVVRQAKPTILIGTSTVSGA
FTEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIEWTEGRALITTGSPFPPVEYNGVTYHIGQANNALVFPGLGLGTI
VTKSKLITDGMFEACARAIAGMVNVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVATEEPEDIIQAVQDAMWYPVY
KPIRAI
>Mature_566_residues
MKQFRVTNEGDIQTTLRGLEVLSVPFLNKGVAFTEEERKELGLKGFLPPKVLTIDDQAKRAYEQYSAQPDDLSKNVYLTA
LHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEYRKPRGLYLSIDDPDGMKEAFKQYKDQSDTIDLIVATDAEG
ILGIGDWGVGGIAISIGKLAVYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARET
FPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHRVVIFGAGTAGIGIAEQLREA
LVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYARSADEVKDYQRNGDGGGIDLLEVVRQAKPTILIGTSTVSGA
FTEEIVKEMASHVKRPAILPMSNPTTLSEAKPEDLIEWTEGRALITTGSPFPPVEYNGVTYHIGQANNALVFPGLGLGTI
VTKSKLITDGMFEACARAIAGMVNVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVATEEPEDIIQAVQDAMWYPVY
KPIRAI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI62420882, Length=549, Percent_Identity=40.8014571948998, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI62420880, Length=549, Percent_Identity=40.8014571948998, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI239049447, Length=549, Percent_Identity=40.8014571948998, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI4505143, Length=536, Percent_Identity=40.1119402985075, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4505145, Length=551, Percent_Identity=38.1125226860254, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI270265879, Length=462, Percent_Identity=40.9090909090909, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI87081919, Length=546, Percent_Identity=44.5054945054945, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17537199, Length=532, Percent_Identity=42.8571428571429, Blast_Score=409, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322823, Length=585, Percent_Identity=41.7094017094017, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI281362672, Length=542, Percent_Identity=39.6678966789668, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281362674, Length=542, Percent_Identity=39.6678966789668, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI21356279, Length=542, Percent_Identity=39.6678966789668, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24646388, Length=545, Percent_Identity=37.6146788990826, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24646386, Length=545, Percent_Identity=37.6146788990826, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281363505, Length=563, Percent_Identity=35.1687388987567, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78707236, Length=563, Percent_Identity=35.1687388987567, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78707232, Length=517, Percent_Identity=36.7504835589942, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281363503, Length=535, Percent_Identity=35.7009345794393, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78707242, Length=546, Percent_Identity=35.8974358974359, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI78707238, Length=546, Percent_Identity=35.8974358974359, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI78707240, Length=522, Percent_Identity=36.3984674329502, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI19922384, Length=556, Percent_Identity=28.9568345323741, Blast_Score=262, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO3_BACSU (O34389)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   D69655
- RefSeq:   NP_390866.1
- ProteinModelPortal:   O34389
- SMR:   O34389
- EnsemblBacteria:   EBBACT00000001690
- GeneID:   938071
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29880
- NMPDR:   fig|224308.1.peg.2991
- GenoList:   BSU29880
- GeneTree:   EBGT00050000001535
- HOGENOM:   HBG289821
- OMA:   IQDNNET
- PhylomeDB:   O34389
- ProtClustDB:   PRK13529
- BioCyc:   BSUB:BSU29880-MONOMER
- BRENDA:   1.1.1.38
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 62149; Mature: 62149

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 105-105 ACT_SITE 178-178 BINDING 273-273 BINDING 423-423

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFRVTNEGDIQTTLRGLEVLSVPFLNKGVAFTEEERKELGLKGFLPPKVLTIDDQAKR
CCCEECCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHH
AYEQYSAQPDDLSKNVYLTALHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEY
HHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGLYLSIDDPDGMKEAFKQYKDQSDTIDLIVATDAEGILGIGDWGVGGIAISIGKLA
CCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHH
VYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARET
HHHHHCCCCHHHEEEEEEECCCCHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
FPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHR
CCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVIFGAGTAGIGIAEQLREALVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYAR
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHCC
SADEVKDYQRNGDGGGIDLLEVVRQAKPTILIGTSTVSGAFTEEIVKEMASHVKRPAILP
CHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
MSNPTTLSEAKPEDLIEWTEGRALITTGSPFPPVEYNGVTYHIGQANNALVFPGLGLGTI
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHH
VTKSKLITDGMFEACARAIAGMVNVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TEEPEDIIQAVQDAMWYPVYKPIRAI
CCCHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MKQFRVTNEGDIQTTLRGLEVLSVPFLNKGVAFTEEERKELGLKGFLPPKVLTIDDQAKR
CCCEECCCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHH
AYEQYSAQPDDLSKNVYLTALHDRNETLFYRLLNDHLGEMLPIVYTPTVGTAIQRYSHEY
HHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGLYLSIDDPDGMKEAFKQYKDQSDTIDLIVATDAEGILGIGDWGVGGIAISIGKLA
CCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHH
VYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARET
HHHHHCCCCHHHEEEEEEECCCCHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
FPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHR
CCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVIFGAGTAGIGIAEQLREALVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYAR
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHCC
SADEVKDYQRNGDGGGIDLLEVVRQAKPTILIGTSTVSGAFTEEIVKEMASHVKRPAILP
CHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
MSNPTTLSEAKPEDLIEWTEGRALITTGSPFPPVEYNGVTYHIGQANNALVFPGLGLGTI
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCHHHH
VTKSKLITDGMFEACARAIAGMVNVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TEEPEDIIQAVQDAMWYPVYKPIRAI
CCCHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377