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The map label for this gene is ppnK

Identifier: 16080006

GI number: 16080006

Start: 3021233

End: 3022036

Strand: Direct

Name: ppnK

Synonym: BSU29540

Alternate gene names: 16080006

Gene position: 3021233-3022036 (Clockwise)

Preceding gene: 16079992

Following gene: 16080012

Centisome position: 71.67

GC content: 44.53

Gene sequence:

>804_bases
ATGACCGATCAACGCCGTAATGTTTATTTTTTTCACAAACAGGATCAGGAAACGAACGAACAAGCCCGCTCGTTAACACA
ATTAGCGGAAGAGCATGGATTTACCGTTGTGAACCAGCACTCAGACGCCAATATTATCGCTAGCATTGGCGGGGACGGCA
CATTTTTGCAGGCAGTCAGAAAGACGAATTTTCGTGACGACTGCTTGTACGTCGGCATTACCAAAAAGGGGAAAGCTCAT
TTATATTGCGACTTTCATAGTGATGAGCGAGAAAAAATGGTCGATGCAATGACGTTTGAACAAATTGAAGTGAGAAAATA
CCCGCTGATTGAAGTGACAGTGGATCAAGCCAGTCCTTTTCACTGCTTAAACGAGGTATCCATCCGCTCAAGCATCATCA
AAACCTTTGTGATGGACGTGCTGATTGACGATCTGCACTTTGAAACGTTTAGAGGTGACGGAATGATTATCTCAACGCCT
ACAGGGAGCACCGCCTATAACAAGTCTGTTGCAGGAGCTGTTGTTGATCCATTGCTTCCATGCATGCAGGTATCAGAGCT
TGCATCCCTTAACAATAATACCTACCGGACACTCGGCTCGCCATTTGTCCTCAGCTCTGACCGAAAGCTGACCCTTCGTG
TGGTGCAGGACGGCAACGAACACCCAATCATCGGCTTGGACAATGAAGCACTCAGCACGAGAAATGTGAAAACGATCGAA
ATCAAACTGTCTAATAAGAAAATCAAAACAGTCAAACTAAAGGACAATTCATTTTGGGAAAAAGTGAAGCGTACTTTTTT
ATAG

Upstream 100 bases:

>100_bases
GGGCTGCCGGCAAACGTGGTACACTTATGGCCAAAGGTATTTTATCACAAATACTGGATGGCAGAAAAACTTAAATATGA
CAGCAAAGGGGAAATGGGAT

Downstream 100 bases:

>100_bases
AAAATAAAAGGACAGGCTGCTGCCTGTCCTTTTATGATTTCTCATATACAATCTGGCCGTTAATCACGGTTTTTTTAATC
TCTAAAAGATGAAGCTGTGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFRDDCLYVGITKKGKAH
LYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTP
TGSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIE
IKLSNKKIKTVKLKDNSFWEKVKRTFL

Sequences:

>Translated_267_residues
MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFRDDCLYVGITKKGKAH
LYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTP
TGSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIE
IKLSNKKIKTVKLKDNSFWEKVKRTFL
>Mature_266_residues
TDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFRDDCLYVGITKKGKAHL
YCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPT
GSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIEI
KLSNKKIKTVKLKDNSFWEKVKRTFL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=227, Percent_Identity=27.3127753303965, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_BACSU (O34934)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   B69990
- RefSeq:   NP_390832.1
- ProteinModelPortal:   O34934
- SMR:   O34934
- EnsemblBacteria:   EBBACT00000002091
- GeneID:   936767
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29540
- NMPDR:   fig|224308.1.peg.2957
- GenoList:   BSU29540
- GeneTree:   EBGT00050000002705
- HOGENOM:   HBG314020
- OMA:   RGDGMII
- ProtClustDB:   PRK03501
- BioCyc:   BSUB:BSU29540-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30254; Mature: 30123

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVR
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHHHH
KTNFRDDCLYVGITKKGKAHLYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQASPF
HCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCCC
HCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVDPLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHH
CMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIE
HHHHHHHHHCCCCCEEECCCCEEEECCCEEEEEEEECCCCCCEEEECCCCCCCCCEEEEE
IKLSNKKIKTVKLKDNSFWEKVKRTFL
EEECCCEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
TDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHHHH
KTNFRDDCLYVGITKKGKAHLYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQASPF
HCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCCC
HCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVDPLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHH
CMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIE
HHHHHHHHHCCCCCEEECCCCEEEECCCEEEEEEEECCCCCCEEEECCCCCCCCCEEEEE
IKLSNKKIKTVKLKDNSFWEKVKRTFL
EEECCCEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377