Click here to switch to the map view.

The map label for this gene is pyk

Identifier: 16079970

GI number: 16079970

Start: 2984788

End: 2986545

Strand: Reverse

Name: pyk

Synonym: BSU29180

Alternate gene names: 16079970

Gene position: 2986545-2984788 (Counterclockwise)

Preceding gene: 16079971

Following gene: 16079969

Centisome position: 70.84

GC content: 45.51

Gene sequence:

>1758_bases
ATGAGAAAAACTAAAATTGTTTGTACCATCGGTCCGGCAAGTGAAAGTATTGAAATGCTTACGAAATTAATGGAGTCAGG
AATGAACGTGGCTCGATTAAACTTTTCTCACGGAGATTTTGAGGAGCACGGTGCAAGAATTAAAAATATCCGCGAAGCAA
GTAAAAAACTTGGCAAGAACGTTGGAATTCTGCTTGATACAAAAGGTCCTGAAATCCGCACACATACAATGGAAAACGGC
GGTATTGAGCTTGAAACAGGCAAAGAGCTCATTATTTCAATGGACGAGGTTGTAGGAACAACAGATAAAATTTCAGTGAC
ATATGAAGGTTTAGTCCATGACGTTGAACAAGGTTCAACGATTCTGTTAGATGACGGCCTTATCGGTCTTGAGGTACTTG
ATGTAGATGCCGCTAAACGCGAAATCAAAACAAAAGTATTAAACAACGGAACACTCAAAAATAAAAAAGGTGTTAACGTA
CCGGGCGTAAGTGTCAATCTTCCGGGGATTACTGAAAAGGATGCGCGAGACATCGTTTTCGGTATTGAGCAAGGAGTAGA
CTTCATCGCACCATCTTTCATTCGACGTTCTACGGATGTGCTCGAAATCCGTGAGCTTCTTGAAGAGCACAACGCTCAGG
ATATTCAAATCATCCCTAAAATCGAAAACCAAGAGGGCGTTGACAACATCGATGCGATTCTCGAAGTGTCTGACGGCTTA
ATGGTTGCACGCGGAGACTTAGGTGTGGAAATTCCAGCTGAAGAAGTGCCGCTTGTGCAAAAAGAACTGATCAAAAAATG
CAACGCGCTGGGCAAACCTGTTATTACAGCGACACAAATGCTTGACAGCATGCAGCGCAACCCGCGTCCGACTCGTGCGG
AAGCAAGTGACGTTGCAAACGCGATCTTCGACGGAACAGATGCGATCATGCTTTCTGGTGAAACTGCTGCCGGAAGTTAC
CCGGTTGAAGCAGTTCAAACAATGCATAACATCGCGTCCCGTTCTGAAGAAGCATTAAATTATAAAGAAATTCTCTCAAA
ACGCAGAGACCAAGTGGGCATGACAATTACAGACGCAATTGGACAATCTGTCGCACATACGGCGATTAACCTGAATGCTG
CTGCGATCGTAACGCCGACAGAAAGCGGCCATACAGCACGTATGATTGCAAAATACCGTCCGCAGGCTCCGATTGTTGCG
GTTACTGTAAATGACTCTATTTCCAGAAAGCTTGCCCTCGTATCTGGCGTATTCGCGGAAAGCGGCCAAAATGCGAGCTC
AACAGATGAGATGCTTGAGGATGCTGTCCAAAAATCATTGAACAGCGGAATTGTAAAACACGGCGATCTTATCGTTATTA
CAGCTGGCACTGTCGGTGAGTCCGGCACTACGAACTTAATGAAGGTTCATACTGTCGGCGATATCATCGCTAAAGGCCAA
GGCATTGGACGCAAATCAGCTTACGGTCCGGTTGTCGTTGCACAAAATGCAAAAGAAGCTGAGCAAAAAATGACTGACGG
TGCGGTACTTGTTACCAAAAGCACTGACCGTGATATGATTGCATCCCTTGAAAAAGCGTCTGCTCTTATTACAGAAGAAG
GCGGTTTGACTAGCCATGCTGCGGTAGTCGGATTAAGCCTTGGCATCCCGGTTATCGTGGGTCTGGAAAATGCGACATCT
ATTTTGACAGATGGCCAGGATATTACAGTTGACGCTTCCAGAGGCGCAGTCTATCAAGGCCGTGCGAGCGTTCTTTAA

Upstream 100 bases:

>100_bases
GACAAAACACACAGTTGAGCAAAACATGTATCAGCTTTCAAAAGAACTGTCTATCTAATGTACAGCTGAAGGCTGAAGAT
TTCAGAAGGAAGTGAACCAA

Downstream 100 bases:

>100_bases
TTACAGGTGAAAATGGAAGGGGAATCCCTTCCTTTTCTCTTTATCATGCCTTTTGTTGAACAGAACGTGTTACACGTGTG
GGGGGCTTGGATATGAGATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHTMENG
GIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGSTILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNV
PGVSVNLPGITEKDARDIVFGIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGSY
PVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAIGQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNDSISRKLALVSGVFAESGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
ILTDGQDITVDASRGAVYQGRASVL

Sequences:

>Translated_585_residues
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHTMENG
GIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGSTILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNV
PGVSVNLPGITEKDARDIVFGIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGSY
PVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAIGQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNDSISRKLALVSGVFAESGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
ILTDGQDITVDASRGAVYQGRASVL
>Mature_585_residues
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHTMENG
GIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGSTILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNV
PGVSVNLPGITEKDARDIVFGIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGSY
PVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAIGQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNDSISRKLALVSGVFAESGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
ILTDGQDITVDASRGAVYQGRASVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=46.530612244898, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=46.530612244898, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=46.3265306122449, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=43.4077079107505, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=43.4077079107505, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=50, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=50, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=47.0588235294118, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=47.0588235294118, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=45.6221198156682, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=52.3206751054852, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=40, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1787994, Length=107, Percent_Identity=42.0560747663551, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=46.7908902691511, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=43.9918533604888, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=43.9918533604888, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=43.9918533604888, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=43.9918533604888, Blast_Score=369, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=43.5051546391753, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=41.9753086419753, Blast_Score=355, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=45.213849287169, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=45.213849287169, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=45.4545454545455, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=33.8144329896907, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24646914, Length=265, Percent_Identity=44.1509433962264, Blast_Score=214, Evalue=2e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_BACSU (P80885)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69685
- RefSeq:   NP_390796.1
- ProteinModelPortal:   P80885
- SMR:   P80885
- PhosSite:   P80885
- EnsemblBacteria:   EBBACT00000004009
- GeneID:   936596
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29180
- NMPDR:   fig|224308.1.peg.2921
- GenoList:   BSU29180
- GeneTree:   EBGT00050000001069
- HOGENOM:   HBG734376
- OMA:   TMENAVE
- PhylomeDB:   P80885
- ProtClustDB:   PRK06354
- BioCyc:   BSUB:BSU29180-MONOMER
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C; SSF52009 PEP_mobile; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 62175; Mature: 62175

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 32-32 BINDING 245-245 BINDING 246-246 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKN
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VGILLDTKGPEIRTHTMENGGIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGST
CEEEEECCCCCEEEEEECCCCEEEECCCEEEEEHHHHHCCCCEEEEEHHHHHHHHCCCCE
ILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNVPGVSVNLPGITEKDARDIVF
EEEECCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
GIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
HHHHCHHHHCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHEECCCE
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGSYPVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHH
GQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVAVTVNDSISRKLALVSGVFAE
HHHHHHHEEECCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
SGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
CCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCEEEEEEHHHHHHCCC
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHEEECCCCCCHHH
AVVGLSLGIPVIVGLENATSILTDGQDITVDASRGAVYQGRASVL
HHEEEECCCEEEEECCCCHHHEECCCEEEEECCCCCEECCCCCCC
>Mature Secondary Structure
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKN
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VGILLDTKGPEIRTHTMENGGIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGST
CEEEEECCCCCEEEEEECCCCEEEECCCEEEEEHHHHHCCCCEEEEEHHHHHHHHCCCCE
ILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNVPGVSVNLPGITEKDARDIVF
EEEECCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
GIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
HHHHCHHHHCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHEECCCE
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGSYPVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHH
GQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVAVTVNDSISRKLALVSGVFAE
HHHHHHHEEECCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
SGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
CCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCEEEEEEHHHHHHCCC
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHA
CCCCCCCCCCEEEECCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHEEECCCCCCHHH
AVVGLSLGIPVIVGLENATSILTDGQDITVDASRGAVYQGRASVL
HHEEEECCCEEEEECCCCHHHEECCCEEEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659