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The map label for this gene is pfkA

Identifier: 16079971

GI number: 16079971

Start: 2986588

End: 2987547

Strand: Reverse

Name: pfkA

Synonym: BSU29190

Alternate gene names: 16079971

Gene position: 2987547-2986588 (Counterclockwise)

Preceding gene: 16079972

Following gene: 16079970

Centisome position: 70.87

GC content: 45.62

Gene sequence:

>960_bases
ATGAAACGAATAGGGGTATTAACGAGCGGCGGGGATTCCCCGGGAATGAACGCAGCAGTTCGCGCAGTAGTCAGAAAAGC
GATCTATCATGACGTTGAAGTTTACGGTATTTACAACGGATACGCGGGATTGATCAGCGGAAAGATTGAAAAGCTTGAAC
TCGGATCAGTAGGCGATATTATACATCGTGGAGGGACTAAGCTTTATACGGCGAGATGTCCTGAATTCAAAACAGTTGAA
GGCCGTGAAAAAGGGATAGCAAACTTGAAGAAGCTTGGTATTGAAGGCCTTGTTGTTATCGGTGGAGACGGTTCCTATAT
GGGTGCGAAAAAATTAACGGAACACGGGTTTCCATGTGTAGGTGTACCGGGTACAATTGATAATGACATTCCGGGCACTG
ATTTTACAATCGGTTTCGATACAGCTTTAAATACAGTAATTGACGCAATTGATAAGATTCGCGATACAGCGACTTCTCAT
GAACGTACATATGTAATCGAAGTAATGGGCCGTCATGCCGGCGATATCGCATTGTGGGCCGGTCTTGCAGGGGGCGCAGA
ATCGATCTTAATCCCTGAGGCAGACTATGACATGCACGAAATCATTGCCCGCTTAAAACGCGGCCACGAACGCGGCAAGA
AGCACAGTATTATTATTGTTGCCGAAGGTGTAGGCAGCGGTGTTGAATTCGGGAAACGCATTGAAGAAGAAACAAATCTT
GAAACTAGGGTATCTGTATTGGGCCATATCCAGCGCGGAGGTTCTCCGAGTGCTGCTGACCGTGTGTTGGCAAGCCGTCT
CGGCGCATATGCAGTTGAACTGCTGCTTGAAGGAAAAGGCGGACGCTGTGTAGGTATACAAAACAATAAGCTTGTAGACC
ATGATATTATAGAAATACTTGAGACAAAACACACAGTTGAGCAAAACATGTATCAGCTTTCAAAAGAACTGTCTATCTAA

Upstream 100 bases:

>100_bases
TAGGTAAGGTTTCATAGGGAGGATGGAGATCCCTTTTCATTGTTTTTAGGGCAATGATCATGTTATGTTTAATCATATAT
GTTGCTGAGGTGAATGGAGA

Downstream 100 bases:

>100_bases
TGTACAGCTGAAGGCTGAAGATTTCAGAAGGAAGTGAACCAAATGAGAAAAACTAAAATTGTTTGTACCATCGGTCCGGC
AAGTGAAAGTATTGAAATGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEILETKHTVEQNMYQLSKELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEILETKHTVEQNMYQLSKELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVE
GREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNL
ETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEILETKHTVEQNMYQLSKELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=42.6512968299712, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=42.3631123919308, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI266453619, Length=350, Percent_Identity=42.5714285714286, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453768, Length=350, Percent_Identity=42.5714285714286, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI266453748, Length=350, Percent_Identity=42.5714285714286, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI4505749, Length=350, Percent_Identity=42.5714285714286, Blast_Score=225, Evalue=3e-59,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=56.2893081761006, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=40.9221902017291, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=41.1003236245955, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI72003601, Length=311, Percent_Identity=32.475884244373, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=39.8255813953488, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=348, Percent_Identity=36.4942528735632, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=41.2103746397695, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=41.6666666666667, Blast_Score=233, Evalue=9e-62,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=41.6666666666667, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34255; Mature: 34255

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCHHHHHCCCCEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCH
ETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEIL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEHHEEECCCCEEEECCCCCCCHHHHHHHH
ETKHTVEQNMYQLSKELSI
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCHHHHHCCCCEEEECCCHHHH
IHRGGTKLYTARCPEFKTVEGREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCV
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCH
ETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEIL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEHHEEECCCCEEEECCCCCCCHHHHHHHH
ETKHTVEQNMYQLSKELSI
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA