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The map label for this gene is ysbB

Identifier: 16079942

GI number: 16079942

Start: 2954492

End: 2955187

Strand: Reverse

Name: ysbB

Synonym: BSU28900

Alternate gene names: 16079942

Gene position: 2955187-2954492 (Counterclockwise)

Preceding gene: 16079943

Following gene: 16079939

Centisome position: 70.1

GC content: 45.55

Gene sequence:

>696_bases
ATGGAAAGCACAATGAGTCCTTATTTTGGGATTGTCGTATCACTTGCTGCATTCGGCATCGGCACCTTTTTATTTAAGAA
AACGAAAGGCTTCTTCCTTTTCACTCCTTTGTTTGTTGCCATGGTGCTGGGGATCGCATTTTTAAAAATCGGCGGTTTTT
CCTACGCGGATTATAATAACGGGGGAGAGATCATTAAGTTTTTTCTTGAACCGGCAACGATCGCATTTGCAATTCCTTTG
TACAAACAAAGAGATAAGCTTAAAAAGTACTGGTGGCAAATCATGGCCTCCATTATTGCCGGCTCTATCTGCTCTGTCAC
GATTGTTTATCTGCTCGCTAAAGGCATTCATCTCGATTCGGCCGTAATGAAAAGCATGCTTCCGCAGGCGGCTACAACGG
CAATTGCGCTTCCTTTATCAAAAGGCATCGGGGGCATCAGCGACATCACAGCCTTTGCGGTCATCTTTAACGCTGTCATC
GTATATGCGCTCGGAGCATTGTTCTTGAAGGTGTTTAAAGTGAAAAATCCGATTTCTAAAGGATTGGCTCTTGGAACATC
CGGACATGCACTTGGTGTAGCGGTCGGGATTGAGATGGGAGAAGTTGAAGCTGCGATGGCAAGTATTGCTGTTGTCGTAG
TAGGAGTGGTAACGGTTCTGGTCATCCCTGTGTTCGTGCAGCTGATCGGAGGATAA

Upstream 100 bases:

>100_bases
AAAACGCAAAACCGAAGCGGACATGAAAACAAAAACAGTGCAAAGCCCGCAGAACAATAACGAACTCGTTCACCATTAAG
GGAAACAGGAGGTTCTTCAC

Downstream 100 bases:

>100_bases
GCCAAGGCTGAATGCCTTGGCTTTTTTTATTTTATGGTGCGTCTTCGGGATCGATATACCCTTGTTTAATGCCCCATTCT
ACTTGATCGTCATGCCACTT

Product: antiholin-like protein LrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MESTMSPYFGIVVSLAAFGIGTFLFKKTKGFFLFTPLFVAMVLGIAFLKIGGFSYADYNNGGEIIKFFLEPATIAFAIPL
YKQRDKLKKYWWQIMASIIAGSICSVTIVYLLAKGIHLDSAVMKSMLPQAATTAIALPLSKGIGGISDITAFAVIFNAVI
VYALGALFLKVFKVKNPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG

Sequences:

>Translated_231_residues
MESTMSPYFGIVVSLAAFGIGTFLFKKTKGFFLFTPLFVAMVLGIAFLKIGGFSYADYNNGGEIIKFFLEPATIAFAIPL
YKQRDKLKKYWWQIMASIIAGSICSVTIVYLLAKGIHLDSAVMKSMLPQAATTAIALPLSKGIGGISDITAFAVIFNAVI
VYALGALFLKVFKVKNPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
>Mature_231_residues
MESTMSPYFGIVVSLAAFGIGTFLFKKTKGFFLFTPLFVAMVLGIAFLKIGGFSYADYNNGGEIIKFFLEPATIAFAIPL
YKQRDKLKKYWWQIMASIIAGSICSVTIVYLLAKGIHLDSAVMKSMLPQAATTAIALPLSKGIGGISDITAFAVIFNAVI
VYALGALFLKVFKVKNPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG

Specific function: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgA, with the holin-like protein CidA. The LrgAB and CidA proteins may affect the proton motive force of the membrane. May be involved in programmed cell

COG id: COG1346

COG function: function code M; Putative effector of murein hydrolase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CidB/LrgB family. LrgB subfamily

Homologues:

Organism=Escherichia coli, GI1788464, Length=199, Percent_Identity=35.1758793969849, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LRGB_BACSU (P94516)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   D69983
- RefSeq:   NP_390768.1
- ProteinModelPortal:   P94516
- EnsemblBacteria:   EBBACT00000002298
- GeneID:   937418
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28900
- NMPDR:   fig|224308.1.peg.2893
- GenoList:   BSU28900
- GeneTree:   EBGT00050000000862
- HOGENOM:   HBG363009
- OMA:   IINFFLE
- PhylomeDB:   P94516
- ProtClustDB:   PRK04288
- BioCyc:   BSUB:BSU28900-MONOMER
- GO:   GO:0019835
- HAMAP:   MF_01142
- InterPro:   IPR007300

Pfam domain/function: PF04172 LrgB

EC number: NA

Molecular weight: Translated: 24398; Mature: 24398

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x117f1784)-; HASH(0x10ac5c3c)-; HASH(0xe6ced10)-; HASH(0x11142860)-; HASH(0x11111384)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTMSPYFGIVVSLAAFGIGTFLFKKTKGFFLFTPLFVAMVLGIAFLKIGGFSYADYNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCC
GGEIIKFFLEPATIAFAIPLYKQRDKLKKYWWQIMASIIAGSICSVTIVYLLAKGIHLDS
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AVMKSMLPQAATTAIALPLSKGIGGISDITAFAVIFNAVIVYALGALFLKVFKVKNPISK
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
GLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
CCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MESTMSPYFGIVVSLAAFGIGTFLFKKTKGFFLFTPLFVAMVLGIAFLKIGGFSYADYNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCC
GGEIIKFFLEPATIAFAIPLYKQRDKLKKYWWQIMASIIAGSICSVTIVYLLAKGIHLDS
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AVMKSMLPQAATTAIALPLSKGIGGISDITAFAVIFNAVIVYALGALFLKVFKVKNPISK
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
GLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
CCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377