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The map label for this gene is araL

Identifier: 16079929

GI number: 16079929

Start: 2944187

End: 2945005

Strand: Reverse

Name: araL

Synonym: BSU28770

Alternate gene names: 16079929

Gene position: 2945005-2944187 (Counterclockwise)

Preceding gene: 16079930

Following gene: 16079928

Centisome position: 69.86

GC content: 49.08

Gene sequence:

>819_bases
ATGCGTATTATGGCCAGTCATGATACGCCTGTGTCACCGGCTGGCATTCTGATTGACTTGGACGGTACTGTATTCAGAGG
AAATGAGTTGATCGAAGGAGCAAGAGAAGCGATCAAAACGCTTAGGAGAATGGGAAAGAAAATCGTCTTTTTAAGCAACC
GGGGGAATATCTCCCGTGCCATGTGCAGAAAAAAACTTCTTGGCGCGGGGATTGAAACGGACGTAAACGACATTGTTCTG
TCATCAAGCGTGACAGCGGCTTTTCTGAAAAAACATTATCGTTTTTCAAAGGTATGGGTGCTTGGGGAGCAAGGCTTGGT
TGACGAGCTGAGGCTGGCCGGTGTGCAGAACGCGAGCGAACCGAAGGAAGCGGATTGGCTCGTGATCTCCCTTCATGAAA
CGCTCACGTACGACGATTTAAATCAAGCCTTTCAAGCGGCTGCCGGCGGCGCTCGTATTATCGCTACAAACAAAGACCGC
TCTTTTCCGAACGAAGACGGAAATGCCATTGATGTGGCCGGAATGATCGGGGCAATTGAGACTTCTGCACAAGCGAAGAC
TGAACTTGTTGTCGGAAAACCGTCATGGCTGATGGCGGAGGCTGCCTGTACAGCAATGGGGCTGTCCGCACATGAATGCA
TGATTATAGGAGACAGCATTGAATCTGACATTGCGATGGGGAAGCTTTATGGCATGAAAAGCGCCTTAGTGCTAACTGGT
TCTGCGAAACAGGGTGAACAGCGTTTGTACACGCCGGATTATGTGCTGGATTCTATTAAGGATGTAACCAAATTGGCTGA
GGAGGGGATTCTGATATGA

Upstream 100 bases:

>100_bases
GTTGCCGAAATGGCCTATCACTCCATTATGCTGAACAAGGATGTAACCCCAATCAATACAGTCCTGCATGAAAAGCATTT
TTATCGAAAACACGGAGCAA

Downstream 100 bases:

>100_bases
ATCGTATCGCAGCTGACGTTCAGCGTGCTTTTGAAAACGCCGGAGAAAAGACGTTGCCTATAAAAGTTGAAGAAATTGTT
CTCGGTAAGCAAGCAGCTGA

Product: phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MRIMASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKIVFLSNRGNISRAMCRKKLLGAGIETDVNDIVL
SSSVTAAFLKKHYRFSKVWVLGEQGLVDELRLAGVQNASEPKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDR
SFPNEDGNAIDVAGMIGAIETSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIAMGKLYGMKSALVLTG
SAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI

Sequences:

>Translated_272_residues
MRIMASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKIVFLSNRGNISRAMCRKKLLGAGIETDVNDIVL
SSSVTAAFLKKHYRFSKVWVLGEQGLVDELRLAGVQNASEPKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDR
SFPNEDGNAIDVAGMIGAIETSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIAMGKLYGMKSALVLTG
SAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI
>Mature_272_residues
MRIMASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKIVFLSNRGNISRAMCRKKLLGAGIETDVNDIVL
SSSVTAAFLKKHYRFSKVWVLGEQGLVDELRLAGVQNASEPKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDR
SFPNEDGNAIDVAGMIGAIETSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIAMGKLYGMKSALVLTG
SAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI

Specific function: Unknown

COG id: COG0647

COG function: function code G; Predicted sugar phosphatases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI10092677, Length=276, Percent_Identity=29.3478260869565, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI108796653, Length=286, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI14149777, Length=244, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI269847098, Length=254, Percent_Identity=25.5905511811024, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786890, Length=252, Percent_Identity=26.1904761904762, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560956, Length=289, Percent_Identity=26.9896193771626, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558880, Length=290, Percent_Identity=26.2068965517241, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562458, Length=289, Percent_Identity=26.9896193771626, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557870, Length=266, Percent_Identity=25.9398496240602, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319965, Length=237, Percent_Identity=23.6286919831224, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24666141, Length=278, Percent_Identity=24.1007194244604, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920940, Length=242, Percent_Identity=27.6859504132231, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24656326, Length=276, Percent_Identity=24.6376811594203, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24656330, Length=254, Percent_Identity=26.3779527559055, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221329670, Length=280, Percent_Identity=26.7857142857143, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARAL_BACSU (P94526)

Other databases:

- EMBL:   X89408
- EMBL:   X89810
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   G69587
- RefSeq:   NP_390755.1
- ProteinModelPortal:   P94526
- SMR:   P94526
- EnsemblBacteria:   EBBACT00000003624
- GeneID:   937431
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28770
- NMPDR:   fig|224308.1.peg.2880
- GenoList:   BSU28770
- GeneTree:   EBGT00050000000481
- HOGENOM:   HBG646794
- OMA:   LVISLHE
- PhylomeDB:   P94526
- ProtClustDB:   CLSK887697
- BioCyc:   BSUB:BSU28770-MONOMER
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006357
- InterPro:   IPR023215
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:3.40.50.10410
- TIGRFAMs:   TIGR01460

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 29315; Mature: 29315

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIMASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKIVFLSNRGNISRA
CEEECCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
MCRKKLLGAGIETDVNDIVLSSSVTAAFLKKHYRFSKVWVLGEQGLVDELRLAGVQNASE
HHHHHHHCCCCCCCHHHEEECCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCC
PKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDRSFPNEDGNAIDVAGMIGAIE
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHH
TSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIAMGKLYGMKSALVLTG
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEC
SAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIMASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKIVFLSNRGNISRA
CEEECCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
MCRKKLLGAGIETDVNDIVLSSSVTAAFLKKHYRFSKVWVLGEQGLVDELRLAGVQNASE
HHHHHHHCCCCCCCHHHEEECCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCC
PKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDRSFPNEDGNAIDVAGMIGAIE
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHH
TSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIAMGKLYGMKSALVLTG
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEC
SAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084180; 8969504; 9384377; 10417639