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The map label for this gene is araM

Identifier: 16079928

GI number: 16079928

Start: 2943006

End: 2944190

Strand: Reverse

Name: araM

Synonym: BSU28760

Alternate gene names: 16079928

Gene position: 2944190-2943006 (Counterclockwise)

Preceding gene: 16079929

Following gene: 255767663

Centisome position: 69.84

GC content: 47.76

Gene sequence:

>1185_bases
ATGAATCGTATCGCAGCTGACGTTCAGCGTGCTTTTGAAAACGCCGGAGAAAAGACGTTGCCTATAAAAGTTGAAGAAAT
TGTTCTCGGTAAGCAAGCAGCTGATTCGCTTTTGGATTATGTAAAACGAAAAAACAATCAACATATTGTCCTTGTCTGCG
ACGCGAATACACACCGCATTGCAGGAATTGATTTAGAAAACCGACTGAATCAAGAAGGATTTCAGGCCGAGTGCCTGATC
ATTCCAGAAAATGAAGCCGGAGATGTGACAGCTGATGAACGATCGCTCATTCATGTGCTGATCCATACGAAACAACCAAC
GGATGTCATGATCGCAGTCGGTTCGGGCACGATTCATGATATCGTCCGCTTTGCGGCGTTTCAAAGAGATTTGCCGTTTA
TTTCTTATCCGACTGCTCCATCTGTAGACGGTTTTACATCAGCCGGTGCGCCGATTATTTTATACGGCACGAAAACAACC
ATTCAAACGAAGGCCCCATCTGCGCTGTTCGCTGATCTGGATCTATTAAAAGCGGCACCGCAGTCAATGGTGGCGGCTGG
CTTTGGTGACATGCTCGGTAAAATCACGTCTTTAGCAGATTGGGAAATATCCCGGCATCTTGCCGGTGAGCCTTATTCGC
CTGCAGGAGCTAAGATCGTTCAGGAGGCGCTTGCTGCCTGCATTGAACACACAGAAGACATTGCGATGAAAACGGAAACT
GGCATACGGGTTTTGATGGAGTCTTTACTTGTATCGGGGCTTGTCATGCTGGCATTAGATCATTCCCGACCGGCATCAGG
CGGCGAGCATCATATTTCACATTGGATTGAAATGGAGTTAATGGAGAAAAAACGGCCTCAGATTCTTCATGGGGCAAAGG
TGGGCTGTGCCGCTGTTTTATTAACTGACACATACAGAAAGCTCGCTCAGGATGACGGGCTGAACGAATTTTCACCAAGC
CGCCGGGAAGCCATCCAATCGGCTTATCAAACACTCCCGAGAGGAGAAGTGCTGGCTGATTGGCTGAGATCAGCCGGAGG
CCCTGCGTATTTTGACGAAATCGGTGTCGGGCAGGATTCCGTCAAAAATGCCTTCAGACACGCGCACACCTTAAGAGACC
GATGCACCGGATTAAGAATCATCAATGAAAACAAAACGCTGATCAACCATGGTCTATATGAATAG

Upstream 100 bases:

>100_bases
CTGGTTCTGCGAAACAGGGTGAACAGCGTTTGTACACGCCGGATTATGTGCTGGATTCTATTAAGGATGTAACCAAATTG
GCTGAGGAGGGGATTCTGAT

Downstream 100 bases:

>100_bases
CCCGCACCTCGAATGGAAGGGGTAACGCAGATGAAAAAAATGACTGTCTGTTTTCTTGTGCTCATGATGTTGCTGACATT
AGTCATTGCCGGGTGTTCAG

Product: metabolite-phosphate dehydrogenase

Products: NA

Alternate protein names: G1P dehydrogenase; G1PDH; Arabinose operon protein AraM; Enantiomeric glycerophosphate synthase; sn-glycerol-1-phosphate dehydrogenase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKNNQHIVLVCDANTHRIAGIDLENRLNQEGFQAECLI
IPENEAGDVTADERSLIHVLIHTKQPTDVMIAVGSGTIHDIVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTT
IQTKAPSALFADLDLLKAAPQSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQEALAACIEHTEDIAMKTET
GIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELMEKKRPQILHGAKVGCAAVLLTDTYRKLAQDDGLNEFSPS
RREAIQSAYQTLPRGEVLADWLRSAGGPAYFDEIGVGQDSVKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE

Sequences:

>Translated_394_residues
MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKNNQHIVLVCDANTHRIAGIDLENRLNQEGFQAECLI
IPENEAGDVTADERSLIHVLIHTKQPTDVMIAVGSGTIHDIVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTT
IQTKAPSALFADLDLLKAAPQSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQEALAACIEHTEDIAMKTET
GIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELMEKKRPQILHGAKVGCAAVLLTDTYRKLAQDDGLNEFSPS
RREAIQSAYQTLPRGEVLADWLRSAGGPAYFDEIGVGQDSVKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE
>Mature_394_residues
MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKNNQHIVLVCDANTHRIAGIDLENRLNQEGFQAECLI
IPENEAGDVTADERSLIHVLIHTKQPTDVMIAVGSGTIHDIVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTT
IQTKAPSALFADLDLLKAAPQSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQEALAACIEHTEDIAMKTET
GIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELMEKKRPQILHGAKVGCAAVLLTDTYRKLAQDDGLNEFSPS
RREAIQSAYQTLPRGEVLADWLRSAGGPAYFDEIGVGQDSVKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE

Specific function: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species. Prefe

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycerol-1-phosphate dehydrogenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): G1PDH_BACSU (P94527)

Other databases:

- EMBL:   X89810
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   H69587
- RefSeq:   NP_390754.1
- ProteinModelPortal:   P94527
- SMR:   P94527
- EnsemblBacteria:   EBBACT00000001156
- GeneID:   938011
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28760
- NMPDR:   fig|224308.1.peg.2879
- GenoList:   BSU28760
- GeneTree:   EBGT00050000001321
- HOGENOM:   HBG313183
- OMA:   TQAPIAL
- ProtClustDB:   CLSK873152
- BioCyc:   BSUB:BSU28760-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00497_B
- InterPro:   IPR002658
- InterPro:   IPR023003

Pfam domain/function: PF01761 DHQ_synthase

EC number: =1.1.1.261

Molecular weight: Translated: 43058; Mature: 43058

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 143-143 BINDING 147-147 BINDING 190-190 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKNNQHIVLVCDANTHRI
CCHHHHHHHHHHHCCCCCCCCEEHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
AGIDLENRLNQEGFQAECLIIPENEAGDVTADERSLIHVLIHTKQPTDVMIAVGSGTIHD
ECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHH
IVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTTIQTKAPSALFADLDLLKAAP
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHCH
QSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQEALAACIEHTEDIAMKTET
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELMEKKRPQILHGAKVGCAAVL
HHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHH
LTDTYRKLAQDDGLNEFSPSRREAIQSAYQTLPRGEVLADWLRSAGGPAYFDEIGVGQDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCCHHH
VKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE
HHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCC
>Mature Secondary Structure
MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKNNQHIVLVCDANTHRI
CCHHHHHHHHHHHCCCCCCCCEEHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
AGIDLENRLNQEGFQAECLIIPENEAGDVTADERSLIHVLIHTKQPTDVMIAVGSGTIHD
ECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHH
IVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTTIQTKAPSALFADLDLLKAAP
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHCH
QSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQEALAACIEHTEDIAMKTET
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELMEKKRPQILHGAKVGCAAVL
HHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHH
LTDTYRKLAQDDGLNEFSPSRREAIQSAYQTLPRGEVLADWLRSAGGPAYFDEIGVGQDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCCHHH
VKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE
HHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9084180; 8969504; 9384377; 10417639