The gene/protein map for NC_000964 is currently unavailable.
Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is folC

Identifier: 16079860

GI number: 16079860

Start: 2865312

End: 2866604

Strand: Reverse

Name: folC

Synonym: BSU28080

Alternate gene names: 16079860

Gene position: 2866604-2865312 (Counterclockwise)

Preceding gene: 255767644

Following gene: 255767643

Centisome position: 68.0

GC content: 43.85

Gene sequence:

>1293_bases
TTGTTTACTGCATATCAAGATGCGCGCAGCTGGATTCACGGGAGGCTGAAATTCGGGGTGAAGCCCGGACTTGGTCGAAT
GAAACAGCTGATGGCGCGGTTAGGACATCCTGAAAAAAAAATCCGAGCGTTCCACGTCGCAGGAACAAACGGTAAAGGAT
CAACTGTCGCTTTTATCCGTTCTATGCTGCAGGAAGCCGGATATACGGTTGGAACATTTACATCGCCTTATATTATTACG
TTTAATGAACGGATCAGCGTAAACGGGATACCGATTTCAGATGAGGAATGGACAGCACTCGTCAATCAAATGAAACCGCA
CGTTGAAGCCCTTGATCAAACGGAATACGGACAGCCGACAGAATTTGAAATTATGACAGCTTGTGCATTTTTATATTTTG
CAGAGTTTCATAAGGTCGATTTTGTTATTTTTGAAACAGGTCTGGGCGGTAGATTCGATTCTACTAATGTGGTTGAACCG
CTCTTAACTGTGATTACAAGCATCGGACACGATCATATGAACATTTTGGGAAACACCATTGAAGAAATTGCAGGAGAAAA
GGCCGGCATTATTAAAGAAGGTATTCCAATCGTTACAGCAGTTACCCAGCCGGAAGCTTTACAGGTCATCCGCCATGAAG
CCGAACGGCATGCTGCGCCGTTCCAGTCATTGCATGATGCATGTGTTATTTTCAATGAAGAGGCTCTGCCTGCAGGAGAA
CAGTTTTCATTCAAAACGGAAGAGAAATGCTATGAAGATATTCGGACATCTCTGATTGGCACCCATCAAAGACAAAATGC
TGCTTTGTCCATTTTGGCTGCGGAGTGGCTCAATAAGGAGAATATCGCCCATATCAGTGACGAAGCGTTAAGGAGCGGGC
TTGTGAAGGCTGCTTGGCCGGGACGGTTAGAGCTTGTTCAGGAACATCCTCCGGTATATTTAGACGGTGCGCATAACGAA
GAAGGTGTTGAAAAGCTGGCGGAAACGATGAAGCAGCGTTTCGCCAATTCACGCATTTCTGTGGTGTTTAGCGCGTTAAA
GGACAAACCGTATCAGAACATGATTAAAAGACTGGAAACCATTGCTCATGCGATTCATTTTGCTTCTTTTGATTTCCCGC
GTGCTTCCCTTGCAAAAGATCTCTATGATGCAAGTGAAATCAGCAATAAAAGCTGGAGTGAAGATCCAGACGATGTAATC
AAATTCATAGAAAGTAAAAAGGGCTCAAATGAAATTGTGCTGATTACCGGATCTCTTTACTTTATTTCTGACATTCGAAA
AAGGTTGAAATAA

Upstream 100 bases:

>100_bases
GTGACGCTGTTCAAAAACGAATGGCTGAGCTGAAGGGTTAATGAAAAGCCGGGGCGGTTTCTAGAGAAACCGCCTTGTCT
TATAAAAGAGGGGATTATCA

Downstream 100 bases:

>100_bases
AAGCATCCGGCTCCGGCAGAATCAAAAAAAGATTCTGCCGTTTTTTTCATGTGTAAAATTGAATATTCCCAATTGGAGCT
CAGGATGTCCATTTTGCTAC

Product: folyl-polyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIIT
FNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEP
LLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGE
QFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNE
EGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVI
KFIESKKGSNEIVLITGSLYFISDIRKRLK

Sequences:

>Translated_430_residues
MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIIT
FNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEP
LLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGE
QFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNE
EGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVI
KFIESKKGSNEIVLITGSLYFISDIRKRLK
>Mature_430_residues
MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIIT
FNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEP
LLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGE
QFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNE
EGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVI
KFIESKKGSNEIVLITGSLYFISDIRKRLK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family

Homologues:

Organism=Homo sapiens, GI66932990, Length=385, Percent_Identity=31.1688311688312, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI66932984, Length=385, Percent_Identity=31.1688311688312, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1788654, Length=403, Percent_Identity=30.0248138957816, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17553150, Length=438, Percent_Identity=28.9954337899543, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71984923, Length=441, Percent_Identity=28.7981859410431, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17553148, Length=441, Percent_Identity=28.7981859410431, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6323760, Length=430, Percent_Identity=27.6744186046512, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324815, Length=328, Percent_Identity=30.1829268292683, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=292, Percent_Identity=23.6301369863014, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641571, Length=319, Percent_Identity=34.4827586206897, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24581568, Length=182, Percent_Identity=34.6153846153846, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLC_BACSU (Q05865)

Other databases:

- EMBL:   L04520
- EMBL:   AL009126
- EMBL:   M30805
- PIR:   B40646
- RefSeq:   NP_390686.1
- ProteinModelPortal:   Q05865
- SMR:   Q05865
- EnsemblBacteria:   EBBACT00000001837
- GeneID:   936327
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28080
- NMPDR:   fig|224308.1.peg.2811
- GenoList:   BSU28080
- GeneTree:   EBGT00050000000631
- HOGENOM:   HBG744947
- OMA:   QRWPETK
- PhylomeDB:   Q05865
- ProtClustDB:   CLSK2460946
- BioCyc:   BSUB:BSU28080-MONOMER
- BRENDA:   6.3.2.17
- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR11136
- TIGRFAMs:   TIGR01499

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.17

Molecular weight: Translated: 48165; Mature: 48165

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHHH
SMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPT
HHHHHCCCEEECCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTI
CHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
EEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGE
HHHCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCC
QFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWP
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHCCCCCCCCC
GRLELVQEHPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLET
CHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHH
IAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLY
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHH
FISDIRKRLK
HHHHHHHHCC
>Mature Secondary Structure
MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHHH
SMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPT
HHHHHCCCEEECCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTI
CHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
EEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGE
HHHCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCC
QFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWP
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHCCCCCCCCC
GRLELVQEHPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLET
CHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHH
IAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLY
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHH
FISDIRKRLK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669