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The map label for this gene is nadB

Identifier: 16079839

GI number: 16079839

Start: 2847871

End: 2849466

Strand: Reverse

Name: nadB

Synonym: BSU27870

Alternate gene names: 16079839

Gene position: 2849466-2847871 (Counterclockwise)

Preceding gene: 16079842

Following gene: 16079838

Centisome position: 67.59

GC content: 46.68

Gene sequence:

>1596_bases
ATGTCTAAAAAGACGATTGCAGTCATCGGTTCAGGGGCGGCGGCACTTTCCTTAGCTGCAGCTTTCCCGCCCTCATACGA
AGTGACTGTTATCACAAAAAAAAGCGTCAAAAACAGCAATTCTGTCTATGCACAAGGCGGGATTGCTGCGGCCTATGCAA
AAGATGACTCGATTGAAGCCCATCTGGAGGATACGTTATACGCAGGGTGCGGCCATAATAATCTAGCTATTGTAGCAGAC
GTATTACATGATGGAAAAATGATGGTTCAAAGCCTATTGGAGCGTGGATTTCCATTCGACCGCAATGAACGAGGCGGTGT
TTGTCTTGGAAGAGAAGGGGCCCACTCATACAACCGCATATTTCATGCAGGCGGAGACGCAACAGGCAGGCTGCTTATCG
ATTATTTGCTCAAACGGATCAACAGCAAAATCAAGTTAATCGAGAATGAAACGGCAGCAGATTTGCTTATAGAGGACGGA
CGATGTATCGGCGTCATGACAAAAGACAGCAAAGGGCGGCTCAAGGTAAGGCACGCAGACGAAGTTGTGCTGGCTGCCGG
CGGCTGTGGAAACCTGTTTCTTCACCATACGAATGATCTCACTGTCACAGGTGACGGTCTTTCTCTGGCTTACCGGGCCG
GGGCGGAATTAACGGATTTGGAGTTTACTCAGTTTCACCCGACACTGCTTGTGAAAAATGGTGTTTCCTACGGGCTTGTT
TCAGAAGCTGTCAGAGGGGAAGGCGGATGTTTAGTAGACGAAAACGGCCGCAGGATTATGGCTGAACGGCATCCTTTAGG
TGACCTGGCTCCAAGAGATATTGTCTCACGGGTTATTCACGAAGAAATGGCAAAAGGAAATCGCGTTTATATAGACTTCA
GTGCGATTTCTGATTTTGAAACGCGTTTCCCTACCATCACCGCTATTTGTGAAAAAGCAGGGATTGATATCCACAGCGGA
AAAATTCCTGTTGCTCCGGGAATGCATTTCTTAATGGGAGGTGTTTCAGTTAATCGCTGGGGAGAAACGACAGTGCCGGG
GCTTTATGCGATTGGCGAGACAGCATGCTCCGGTTTACATGGAGCAAACCGGCTTGCAAGCAATTCTTTATTGGAAGCGC
TGGTATTTGGAAAAAGGGCAGCAGAGCATATTATCCAAAAACCGGTTTATAACAGGCAATATCAATCAGGGCTAGAAACC
AGTGTCTTCTACGAGGTGCCGGATATAGAGGGGCATGAACTGCAAAGCAAAATGACAAGCCACATGTCTATTCTGCGGGA
ACAAAGCAGTTTGATTGAGCTTAGCATCTGGCTTCATACGCTGCCTTTTCAGGAAGTAAATGTGAAGGATATCACAATTC
GGCAGATGGAACTTTCTCATCTATGGCAAACTGCAAAGCTGATGACGTTTTCTGCGCTTTTGCGGGAGGAAAGCAGAGGG
GCTCACTTCCGCACCGATTTTCCTCATGCTGAGGTGAGCTGGCAAGGAAGACAAATTGTCCATACAAAAAAAGGAACGAA
GATCAGAAAAAACGAGGGGATTTGGAACAATGAATCATTTACAGCTGAAAAAATTACTGAATCACTTTTTTCTTGA

Upstream 100 bases:

>100_bases
GGTCTTCCTCCATCCGTTCTCCATAAAAAACTCTTGAGTTTATTTTATCCTTGTGTAAATATAGGTGTCAAGACAGGTGT
AAACAACAGGAGGATGGCAT

Downstream 100 bases:

>100_bases
GGATATTGGGACAGGGGATCTTACATCGCAGTCTATTTTTGGAGAACAGAGCTGTGAAGCGGAGATTGTTGCAAAGTCAG
AGGGTATTTTTGCAGGAGCA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQGGIAAAYAKDDSIEAHLEDTLYAGCGHNNLAIVAD
VLHDGKMMVQSLLERGFPFDRNERGGVCLGREGAHSYNRIFHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDG
RCIGVMTKDSKGRLKVRHADEVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEFTQFHPTLLVKNGVSYGLV
SEAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHEEMAKGNRVYIDFSAISDFETRFPTITAICEKAGIDIHSG
KIPVAPGMHFLMGGVSVNRWGETTVPGLYAIGETACSGLHGANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLET
SVFYEVPDIEGHELQSKMTSHMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMELSHLWQTAKLMTFSALLREESRG
AHFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNNESFTAEKITESLFS

Sequences:

>Translated_531_residues
MSKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQGGIAAAYAKDDSIEAHLEDTLYAGCGHNNLAIVAD
VLHDGKMMVQSLLERGFPFDRNERGGVCLGREGAHSYNRIFHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDG
RCIGVMTKDSKGRLKVRHADEVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEFTQFHPTLLVKNGVSYGLV
SEAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHEEMAKGNRVYIDFSAISDFETRFPTITAICEKAGIDIHSG
KIPVAPGMHFLMGGVSVNRWGETTVPGLYAIGETACSGLHGANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLET
SVFYEVPDIEGHELQSKMTSHMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMELSHLWQTAKLMTFSALLREESRG
AHFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNNESFTAEKITESLFS
>Mature_530_residues
SKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQGGIAAAYAKDDSIEAHLEDTLYAGCGHNNLAIVADV
LHDGKMMVQSLLERGFPFDRNERGGVCLGREGAHSYNRIFHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDGR
CIGVMTKDSKGRLKVRHADEVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEFTQFHPTLLVKNGVSYGLVS
EAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHEEMAKGNRVYIDFSAISDFETRFPTITAICEKAGIDIHSGK
IPVAPGMHFLMGGVSVNRWGETTVPGLYAIGETACSGLHGANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLETS
VFYEVPDIEGHELQSKMTSHMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMELSHLWQTAKLMTFSALLREESRGA
HFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNNESFTAEKITESLFS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=519, Percent_Identity=31.7919075144509, Blast_Score=192, Evalue=9e-49,
Organism=Escherichia coli, GI1788928, Length=512, Percent_Identity=38.28125, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1790597, Length=510, Percent_Identity=31.7647058823529, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786942, Length=519, Percent_Identity=31.4065510597302, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17505833, Length=519, Percent_Identity=30.8285163776493, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17550100, Length=520, Percent_Identity=33.2692307692308, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71986328, Length=455, Percent_Identity=25.4945054945055, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=536, Percent_Identity=30.2238805970149, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322416, Length=540, Percent_Identity=30.3703703703704, Blast_Score=193, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6320788, Length=478, Percent_Identity=27.4058577405858, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322511, Length=479, Percent_Identity=27.1398747390397, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137288, Length=520, Percent_Identity=31.9230769230769, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655642, Length=520, Percent_Identity=31.9230769230769, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655647, Length=520, Percent_Identity=31.9230769230769, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24663005, Length=520, Percent_Identity=30, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_BACSU (P38032)

Other databases:

- EMBL:   M98822
- EMBL:   AL009126
- PIR:   C47071
- RefSeq:   NP_390665.1
- ProteinModelPortal:   P38032
- SMR:   P38032
- EnsemblBacteria:   EBBACT00000001646
- GeneID:   938051
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27870
- NMPDR:   fig|224308.1.peg.2790
- GenoList:   BSU27870
- GeneTree:   EBGT00050000000837
- HOGENOM:   HBG293998
- OMA:   HLWQTAK
- PhylomeDB:   P38032
- ProtClustDB:   PRK08071
- BioCyc:   BSUB:BSU27870-MONOMER
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 58240; Mature: 58109

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: ACT_SITE 226-226 ACT_SITE 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQGGIAAAYAKDDSIEA
CCCCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCEEEEEECCCCCHH
HLEDTLYAGCGHNNLAIVADVLHDGKMMVQSLLERGFPFDRNERGGVCLGREGAHSYNRI
HCCHHEEECCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHE
FHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDGRCIGVMTKDSKGRLKVRHAD
EECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCEEEEECC
EVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEFTQFHPTLLVKNGVSYGLV
EEEEEECCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCEEHCCCCEEEEECCCCHHHH
SEAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHEEMAKGNRVYIDFSAISDFE
HHHHCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
TRFPTITAICEKAGIDIHSGKIPVAPGMHFLMGGVSVNRWGETTVPGLYAIGETACSGLH
HCCCHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCC
GANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLETSVFYEVPDIEGHELQSKMTS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHH
HMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMELSHLWQTAKLMTFSALLREESRG
HHHHHHHHHHEEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
AHFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNNESFTAEKITESLFS
CCEECCCCCCEECCCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQGGIAAAYAKDDSIEA
CCCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCEEEEEECCCCCHH
HLEDTLYAGCGHNNLAIVADVLHDGKMMVQSLLERGFPFDRNERGGVCLGREGAHSYNRI
HCCHHEEECCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHE
FHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDGRCIGVMTKDSKGRLKVRHAD
EECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCEEEEECC
EVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEFTQFHPTLLVKNGVSYGLV
EEEEEECCCCCEEEEECCCEEEECCCCEEEEECCCCCCCCEEHCCCCEEEEECCCCHHHH
SEAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHEEMAKGNRVYIDFSAISDFE
HHHHCCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
TRFPTITAICEKAGIDIHSGKIPVAPGMHFLMGGVSVNRWGETTVPGLYAIGETACSGLH
HCCCHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCC
GANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLETSVFYEVPDIEGHELQSKMTS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHH
HMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMELSHLWQTAKLMTFSALLREESRG
HHHHHHHHHHEEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
AHFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNNESFTAEKITESLFS
CCEECCCCCCEECCCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377