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The map label for this gene is queA

Identifier: 16079825

GI number: 16079825

Start: 2833899

End: 2834927

Strand: Reverse

Name: queA

Synonym: BSU27720

Alternate gene names: 16079825

Gene position: 2834927-2833899 (Counterclockwise)

Preceding gene: 255767633

Following gene: 16079824

Centisome position: 67.25

GC content: 45.77

Gene sequence:

>1029_bases
ATGAAAGTCGATTTATTTGATTTTGAACTACCAGAACGTTTAATTGCACAAGTACCGTTGGAGCAGCGTGATGCTTCAAG
GCTGATGGTGCTCGATAAACATACAGGAGAGCTTACTGACAGCTCGTTTAAACACATTATCAGCTTCTTTAACGAAGGCG
ATTGTCTAGTCCTGAACAATACCCGGGTACTGCCTGCGCGGTTATTCGGCACAAAAGAGGATACCGGCGCGAAAGTGGAA
CTTCTTTTGTTGAAACAGGAAACCGGTGATAAATGGGAAACACTTGCCAAACCGGCGAAACGGGTGAAAAAAGGAACTGT
TGTCACATTTGGAGACGGAAGATTGAAGGCAATCTGCACGGAAGAGCTTGAGCACGGCGGCAGAAAGATGGAATTTCAAT
ATGACGGTATTTTCTACGAGGTGCTTGAATCCCTTGGAGAGATGCCGCTCCCTCCATATATCAAAGAACAGCTTGATGAC
AAAGAGCGCTACCAGACAGTGTATTCAAAAGAAATTGGCTCTGCTGCTGCGCCTACAGCTGGCCTTCACTTCACGGAAGA
AATCTTACAGCAGCTGAAAGACAAAGGCGTGCAAATTGAGTTCATCACACTGCACGTAGGCCTTGGAACGTTTCGCCCTG
TGAGTGCCGATGAAGTGGAAGAGCATAATATGCATGCAGAGTTTTATCAAATGTCAGAAGAAACGGCTGCAGCCTTAAAC
AAAGTAAGAGAAAATGGCGGTCGGATCATTTCTGTCGGCACGACCTCGACACGCACGCTTGAGACGATTGCCGGAGAACA
TGACGGGCAATTTAAAGCATCAAGCGGCTGGACATCTATTTTTATTTATCCGGGCTATGAATTTAAAGCCATTGACGGAA
TGATTACAAACTTCCATTTGCCGAAATCGTCATTGATTATGCTGGTCAGCGCGCTTGCTGGAAGAGAAAACATTCTGCGT
GCCTATAACCATGCAGTGGAAGAGGAATACCGTTTCTTCAGCTTCGGAGACGCGATGCTGATTATATAA

Upstream 100 bases:

>100_bases
CGGTTGTCACTTGTATTATTATAAGTGTGGTATTATCAATTTTGCTTAATCTTTTTGGCAGAATGAAATAACAAAAGACT
AGAATAAAGGTGAAATCATC

Downstream 100 bases:

>100_bases
TTTGAGAACAGGAGGCCTCATCAAAAGTGGCTGAACAACCAATACGCTATGAATTTATAAAAGAATGCAAACAAACAGGA
GCGCGGCTGGGCAAAGTGCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICTEELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVYSKEIGSAAAPTAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALN
KVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGRENILR
AYNHAVEEEYRFFSFGDAMLII

Sequences:

>Translated_342_residues
MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICTEELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVYSKEIGSAAAPTAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALN
KVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGRENILR
AYNHAVEEEYRFFSFGDAMLII
>Mature_342_residues
MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICTEELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVYSKEIGSAAAPTAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALN
KVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGRENILR
AYNHAVEEEYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=45.7971014492754, Blast_Score=307, Evalue=7e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACSU (O32054)

Other databases:

- EMBL:   Y15896
- EMBL:   AL009126
- PIR:   A69688
- RefSeq:   NP_390650.1
- PDB:   1YY3
- PDBsum:   1YY3
- ProteinModelPortal:   O32054
- SMR:   O32054
- EnsemblBacteria:   EBBACT00000003591
- GeneID:   937975
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27720
- NMPDR:   fig|224308.1.peg.2775
- GenoList:   BSU27720
- GeneTree:   EBGT00050000002867
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- PhylomeDB:   O32054
- ProtClustDB:   PRK00147
- BioCyc:   BSUB:BSU27720-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38512; Mature: 38512

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNN
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLFGTKEDTGAKVELLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICT
CEEEEHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCEEHEEH
EELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVYSKEIGSAAAPTA
HHHHCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
GLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHL
HHHHCCCEEEEECCCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEECCCEEEEECC
PKSSLIMLVSALAGRENILRAYNHAVEEEYRFFSFGDAMLII
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCEEEECCEEEEC
>Mature Secondary Structure
MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNN
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLFGTKEDTGAKVELLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICT
CEEEEHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCEEHEEH
EELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVYSKEIGSAAAPTA
HHHHCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
GLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHL
HHHHCCCEEEEECCCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEECCCEEEEECC
PKSSLIMLVSALAGRENILRAYNHAVEEEYRFFSFGDAMLII
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377