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The map label for this gene is tgt

Identifier: 16079824

GI number: 16079824

Start: 2832727

End: 2833872

Strand: Reverse

Name: tgt

Synonym: BSU27710

Alternate gene names: 16079824

Gene position: 2833872-2832727 (Counterclockwise)

Preceding gene: 16079825

Following gene: 255767632

Centisome position: 67.22

GC content: 47.12

Gene sequence:

>1146_bases
GTGGCTGAACAACCAATACGCTATGAATTTATAAAAGAATGCAAACAAACAGGAGCGCGGCTGGGCAAAGTGCATACGCC
TCACGGCTCCTTCGAAACGCCGGTTTTTATGCCGGTCGGCACACTGGCGACAGTCAAAACAATGTCGCCTGAAGAGCTGA
AAGCAATGGATGCAGGCATCATTTTGAGCAACACTTATCATCTATGGCTTCGTCCGGGCCAAGACATTGTCAAAGAAGCG
GGCGGGCTCCATAAATTTATGAACTGGGACCGCGCAATTTTAACAGATTCAGGCGGTTTTCAGGTCTTTTCTTTAAGCAA
GTTTCGTAATATAGAGGAAGAGGGTGTCCATTTCCGCAATCATCTAAATGGAGACAAGCTGTTTTTATCTCCGGAAAAAG
CGATGGAGATCCAAAACGCACTCGGCTCTGATATTATGATGGCGTTTGACGAATGCCCGCCATACCCGGCGGAGTACGAC
TACATGAAGAGGTCAGTTGAACGGACAAGCCGCTGGGCGGAACGCTGCCTGAATGCCCACAATCGTCAGGATGAACAAGG
GCTGTTTGGTATTGTACAAGGCGGAGAATATGAAGATCTGCGGACACAAAGTGCGAAGGACTTGATTTCGCTTGATTTTC
CGGGATATGCTATAGGCGGATTGTCTGTAGGTGAACCGAAGGATGTCATGAACCGTGTACTCGAATTCACAACACCGCTC
TTGCCGAAGGATAAACCGAGATATCTGATGGGAGTAGGTTCTCCGGACGCACTGATCGACGGAGCGATCCGCGGGGTCGA
TATGTTTGACTGTGTGCTGCCTACACGGATTGCAAGAAACGGCACAGTGTTTACTGCTGAAGGACGACTCAATATGAAAA
ATGCGAAATTTGAAAGAGACTTCCGTCCGATTGATGAAGAGTGTGATTGCTATACATGTAAAAACTACACTCGCGCGTAC
ATTAGGCACTTGATTCGCTGCAATGAAACATTCGGCCTTCGATTAACAACTTACCATAACCTTCATTTTCTGTTACACTT
AATGGAGCAGGTAAGACAGGCTATTCGCGAGGATCGTCTGGGTGATTTCAGAGAAGAGTTCTTTGAGCGTTACGGCTATA
ACAAGCCAAATGCGAAAAGCTTCTAA

Upstream 100 bases:

>100_bases
TGCGTGCCTATAACCATGCAGTGGAAGAGGAATACCGTTTCTTCAGCTTCGGAGACGCGATGCTGATTATATAATTTGAG
AACAGGAGGCCTCATCAAAA

Downstream 100 bases:

>100_bases
ATAAACATAACAGGCTTACAGAAAGGGGTGAAAATGATGACTGGCACTTTAGGTACATTGGTTCCTATTATTTTAATGTT
TGCGGTCCTTTACTTCTTGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGIILSNTYHLWLRPGQDIVKEA
GGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKRSVERTSRWAERCLNAHNRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERDFRPIDEECDCYTCKNYTRAY
IRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF

Sequences:

>Translated_381_residues
MAEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGIILSNTYHLWLRPGQDIVKEA
GGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKRSVERTSRWAERCLNAHNRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERDFRPIDEECDCYTCKNYTRAY
IRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF
>Mature_380_residues
AEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGIILSNTYHLWLRPGQDIVKEAG
GLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDY
MKRSVERTSRWAERCLNAHNRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPLL
PKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERDFRPIDEECDCYTCKNYTRAYI
RHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=45.9239130434783, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=26.2237762237762, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=57.3816155988858, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=49.8607242339833, Blast_Score=337, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI25146502, Length=245, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=45.9677419354839, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=25.78125, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43711; Mature: 43580

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGI
CCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHHEECCCHHHHHHHHCCE
ILSNTYHLWLRPGQDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRN
EEECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLNAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
CCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHCC
FRPIDEECDCYTCKNYTRAYIRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
GDFREEFFERYGYNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGI
CCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHHEECCCHHHHHHHHCCE
ILSNTYHLWLRPGQDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRN
EEECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLNAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
CCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHCC
FRPIDEECDCYTCKNYTRAYIRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
GDFREEFFERYGYNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA