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The map label for this gene is mccA

Identifier: 16079780

GI number: 16079780

Start: 2786142

End: 2787065

Strand: Reverse

Name: mccA

Synonym: BSU27260

Alternate gene names: 16079780

Gene position: 2787065-2786142 (Counterclockwise)

Preceding gene: 16079781

Following gene: 16079779

Centisome position: 66.11

GC content: 46.43

Gene sequence:

>924_bases
ATGACAGTGATTACGGACATTACCGAGCTGATTGGGAATACACCGCTTCTCCGCTTGAAGAACTTTGATGTTCCAGAAGG
AGTCGCGGTGTATGCCAAGCTTGAAATGATGAATCCAGGGGGAAGCATCAAAGACAGACTGGGAGATATGCTGATTCGTG
ACGCGCTCGATTCAGGCAAAGTGAAACCCGGCGGAGTGATTATAGAAGCTACGGCAGGCAATACAGGGATCGGCCTTGCG
TTAAGTGCCAGAAAATATGGGCTGAAAGCGATTTTCTGTGTCCCTGAACATTTCAGCAGAGAGAAACAGCAAATTATGCA
AGCGCTTGGCGCATCAATCATTCATACACCGCGTCAAGACGGAATGCAGGGAGCCATTCAAAAAGCCATTCAGCTGGAGA
CTGAAATTGAAAATTCTTATTGCGTTTTGCAATTTAAAAACCGAGTAAATCCATCGACTTACTATAAGACGCTTGGACCT
GAAATGTGGGAAGCGCTTGACGGGAATATTCATACATTTGTCGCAGGGGCAGGATCAGGAGGCACATTCGCAGGGACAGC
TTCCTTCTTAAAAGAAAAAAATCCAGCAGTCAAAACGGTGATTGTTGAACCGGTGGGCTCGATTTTAAACGGAGGAGAGC
CCCACGCTCATAAAACAGAGGGCATCGGCATGGAGTTTATCCCCGATTATATGGATAAGAGCCATTTTGACGAGATCTAC
ACCGTAACAGATGAGAATGCGTTTAGACTTGTGAAAGAAGCGGCAGAAAAAGAAGGCCTGCTGATCGGAAGCTCTTCAGG
TGCAGCGCTTTATGCGGCATTGGAGGAAGCGAAAAAAGCATCCGCTGGAACTAACATTGTCACAGTATTTCCTGACAGCA
GCGATCGATATATCAGCAAACAAATATACGAAGGAGGCATCTGA

Upstream 100 bases:

>100_bases
GAGCTTGTGTTAAAGGTGATCAAACGAATTCATTAAAACGGGGAGAACGTTCTCCTCCGTTTTTCTCTAAAAACAACCTC
TATTAAGGAGGAGGACATAT

Downstream 100 bases:

>100_bases
TATGAAGAAAAAAACATTGATGATACATGGCGGAATCACAGGTGATGAGAAAACAGGCGCAGTTTCCGTGCCGATTTATC
AAGTAAGCACGTACAAGCAG

Product: cystathionine beta-synthase for the reverse transsulfuration pathway

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLA
LSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGP
EMWEALDGNIHTFVAGAGSGGTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIY
TVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPDSSDRYISKQIYEGGI

Sequences:

>Translated_307_residues
MTVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLA
LSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGP
EMWEALDGNIHTFVAGAGSGGTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIY
TVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPDSSDRYISKQIYEGGI
>Mature_306_residues
TVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLAL
SARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGPE
MWEALDGNIHTFVAGAGSGGTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIYT
VTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPDSSDRYISKQIYEGGI

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=38.8349514563107, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=38.8349514563107, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=38.8349514563107, Blast_Score=218, Evalue=4e-57,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=38.4615384615385, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=35.2159468438538, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=40.9395973154362, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17562970, Length=304, Percent_Identity=34.8684210526316, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=35.1170568561873, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=38.7959866220736, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17561720, Length=304, Percent_Identity=35.1973684210526, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=37.1794871794872, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=35.6687898089172, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=34.4339622641509, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=38.0952380952381, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=34.0490797546012, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=39.1585760517799, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=39.1585760517799, Blast_Score=201, Evalue=5e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): MCCA_BACSU (O05393)

Other databases:

- EMBL:   U93874
- EMBL:   AL009126
- PIR:   H69973
- RefSeq:   NP_390604.1
- HSSP:   P35520
- ProteinModelPortal:   O05393
- SMR:   O05393
- EnsemblBacteria:   EBBACT00000003385
- GeneID:   937576
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27260
- NMPDR:   fig|224308.1.peg.2729
- GenoList:   BSU27260
- GeneTree:   EBGT00070000031817
- HOGENOM:   HBG748215
- OMA:   ANSHIVT
- PhylomeDB:   O05393
- ProtClustDB:   CLSK2752460
- BioCyc:   BSUB:BSU27260-MONOMER
- InterPro:   IPR001216
- InterPro:   IPR001926

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: 2.5.1.47

Molecular weight: Translated: 33019; Mature: 32888

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 74-74 BINDING 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGK
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
VKPGGVIIEATAGNTGIGLALSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQD
CCCCCEEEEECCCCCCEEEEEECHHCCCEEEEECCHHHHHHHHHHHHHHCHHHCCCCCCC
GMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGPEMWEALDGNIHTFVAGAGSG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEEEEEECCCC
GTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIY
CCCHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHCCCEE
TVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPDSSDRYISK
EECCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
QIYEGGI
HHHCCCC
>Mature Secondary Structure 
TVITDITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGK
CHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
VKPGGVIIEATAGNTGIGLALSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQD
CCCCCEEEEECCCCCCEEEEEECHHCCCEEEEECCHHHHHHHHHHHHHHCHHHCCCCCCC
GMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGPEMWEALDGNIHTFVAGAGSG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEEEEEECCCC
GTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIY
CCCHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHCCCEE
TVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPDSSDRYISK
EECCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
QIYEGGI
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 3.6 {S2-}} 0.1 {S2-}} 0.12 {acetyl-CoA}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377