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The map label for this gene is yrhB

Identifier: 16079779

GI number: 16079779

Start: 2785001

End: 2786140

Strand: Reverse

Name: yrhB

Synonym: BSU27250

Alternate gene names: 16079779

Gene position: 2786140-2785001 (Counterclockwise)

Preceding gene: 16079780

Following gene: 16079778

Centisome position: 66.09

GC content: 46.67

Gene sequence:

>1140_bases
ATGAAGAAAAAAACATTGATGATACATGGCGGAATCACAGGTGATGAGAAAACAGGCGCAGTTTCCGTGCCGATTTATCA
AGTAAGCACGTACAAGCAGCCGAAAGCAGGGCAGCATACAGGCTACGAGTATTCAAGAACGGCCAATCCGACTCGAACCG
CTCTCGAAGCACTTGTGACAGAACTGGAAAGCGGGGAAGCAGGCTATGCGTTCAGCTCAGGAATGGCTGCCATTACAGCG
GTTATGATGCTGTTTAACAGCGGAGATCATGTCGTGTTGACTGATGATGTGTACGGCGGAACATATCGCGTGATGACAAA
GGTGCTTAACCGTCTTGGCATTGAATCAACATTTGTTGATACGAGCAGCAGGGAAGAAGTTGAAAAAGCGATTCGCCCTA
ATACAAAAGCAATTTATATTGAAACACCGACAAACCCGTTGCTCAAAATCACCGACCTGACGCTCATGGCTGATATCGCA
AAAAAAGCGGGTGTTCTGCTTATCGTAGACAATACCTTTAATACTCCTTATTTTCAACAGCCGCTTACTTTAGGCGCTGA
TATCGTACTGCACAGTGCGACAAAATATCTTGGCGGACACAGTGATGTCGTCGGAGGTTTAGTTGTGACAGCTTCGAAAG
AGCTTGGAGAAGAGCTGCATTTTGTGCAAAACTCCACAGGCGGCGTGCTCGGCCCTCAAGATTCCTGGCTGTTAATGAGA
GGAATCAAAACGTTGGGACTCAGAATGGAAGCGATCGATCAAAATGCGCGGAAAATCGCAAGCTTTCTTGAGAATCACCC
TGCTGTCCAAACGTTATATTACCCTGGTTCTTCAAATCATCCCGGACATGAGCTTGCAAAAACGCAAGGAGCGGGCTTCG
GCGGCATGATCTCCTTTGATATTGGCAGTGAAGAACGGGTTGATGCGTTTTTAGGAAATCTGAAACTGTTTACCATTGCT
GAAAGCCTGGGGGCGGTTGAAAGCTTAATTTCTGTTCCTGCAAGAATGACACATGCCTCTATTCCGAGAGAACGCCGGCT
TGAGCTCGGCATTACGGACGGCTTGATCAGAATTTCTGTAGGAATTGAAGATGCGGAAGACTTGTTGGAAGATATCGGCC
AAGCGCTTGAAAATATATAA

Upstream 100 bases:

>100_bases
GAAGCGAAAAAAGCATCCGCTGGAACTAACATTGTCACAGTATTTCCTGACAGCAGCGATCGATATATCAGCAAACAAAT
ATACGAAGGAGGCATCTGAT

Downstream 100 bases:

>100_bases
CAAATGGATAGAATTACTTTCTCTGTAAGCCGCCGGATTATATTATAATAGTCGTATACTATGAATCTGGCAGGTGATTC
TTTTGAACAAAAATAGAATG

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVTELESGEAGYAFSSGMAAITA
VMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIA
KKAGVLLIVDNTFNTPYFQQPLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR
GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFDIGSEERVDAFLGNLKLFTIA
ESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISVGIEDAEDLLEDIGQALENI

Sequences:

>Translated_379_residues
MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVTELESGEAGYAFSSGMAAITA
VMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIA
KKAGVLLIVDNTFNTPYFQQPLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR
GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFDIGSEERVDAFLGNLKLFTIA
ESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISVGIEDAEDLLEDIGQALENI
>Mature_379_residues
MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVTELESGEAGYAFSSGMAAITA
VMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIA
KKAGVLLIVDNTFNTPYFQQPLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR
GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFDIGSEERVDAFLGNLKLFTIA
ESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISVGIEDAEDLLEDIGQALENI

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=376, Percent_Identity=50, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI299473759, Length=376, Percent_Identity=46.5425531914894, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI25453487, Length=375, Percent_Identity=44, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790375, Length=373, Percent_Identity=42.627345844504, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=27.2264631043257, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=49.1620111731844, Blast_Score=343, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=47.3544973544974, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=42.4324324324324, Blast_Score=296, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=48.3116883116883, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=34.8370927318296, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=35.8796296296296, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321170, Length=288, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321904, Length=343, Percent_Identity=33.2361516034985, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322970, Length=357, Percent_Identity=26.890756302521, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323557, Length=329, Percent_Identity=26.1398176291793, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322590, Length=259, Percent_Identity=25.0965250965251, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922554, Length=377, Percent_Identity=48.2758620689655, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MCCB_BACSU (O05394)

Other databases:

- EMBL:   U93874
- EMBL:   AL009126
- PIR:   A69974
- RefSeq:   NP_390603.1
- HSSP:   P31373
- ProteinModelPortal:   O05394
- SMR:   O05394
- EnsemblBacteria:   EBBACT00000003482
- GeneID:   936947
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27250
- NMPDR:   fig|224308.1.peg.2728
- GenoList:   BSU27250
- GeneTree:   EBGT00070000031827
- HOGENOM:   HBG754729
- OMA:   NQYEYSR
- PhylomeDB:   O05394
- ProtClustDB:   PRK07671
- BioCyc:   BSUB:BSU27250-MONOMER
- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.4.1.1; =4.4.1.2

Molecular weight: Translated: 40886; Mature: 40886

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVT
CCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELESGEAGYAFSSGMAAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVD
HHCCCCCCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEC
TSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQ
CCCHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
PLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR
CHHHCHHHHHHHHHHHHCCCCHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEE
IGSEERVDAFLGNLKLFTIAESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISV
CCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHCEEECCCCCEEEEEE
GIEDAEDLLEDIGQALENI
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVT
CCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELESGEAGYAFSSGMAAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVD
HHCCCCCCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEC
TSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQ
CCCHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
PLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR
CHHHCHHHHHHHHHHHHCCCCHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEE
IGSEERVDAFLGNLKLFTIAESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISV
CCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHCEEECCCCCEEEEEE
GIEDAEDLLEDIGQALENI
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377