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The map label for this gene is cwlA

Identifier: 16079643

GI number: 16079643

Start: 2664573

End: 2665391

Strand: Reverse

Name: cwlA

Synonym: BSU25900

Alternate gene names: 16079643

Gene position: 2665391-2664573 (Counterclockwise)

Preceding gene: 16079644

Following gene: 16079642

Centisome position: 63.23

GC content: 40.66

Gene sequence:

>819_bases
ATGGCCATTAAAGTTGTAAAGAATCTAGTCTCTAAATCAAAGTATGGATTGAAATGTCCTAATCCAATGAAAGCTGAATA
TATCACTATTCATAACACTGCGAATGATGCTTCAGCAGCCAATGAGATTTCTTACATGAAGAATAACTCTAGCTCAACAA
GTTTTCACTTTGCAGTAGACGATAAACAAGTCATTCAAGGTATTCCAACGAATCGTAACGCTTGGCACACAGGAGATGGA
ACAAACGGTACAGGGAATCGCAAGTCTATTGGTGTCGAAATTTGTTATAGCAAGTCAGGAGGGGTACGATATAAGGCAGC
GGAAAAGCTTGCTATTAAGTTTGTGGCTCAGCTACTTAAAGAACGTGGATGGGGTATTGATCGAGTCCGCAAACATCAAG
ACTGGAATGGTAAGTATTGCCCGCACCGCATTTTGTCAGAGGGAAGATGGATTCAAGTTAAGACTGCAATTGAAGCAGAA
TTGAAAAAGTTGGGCGGAAAAACAAACTCAAGCAAAGCAAGTGTAGCTAAAAAGAAAACAACAAACACAAGCAGCAAAAA
AACGTCATATGCGCTACCATCCGGTATTTTTAAAGTGAAGAGCCCAATGATGAGAGGGGAAAAGGTAACACAAATTCAAA
AAGCACTGGCTGCACTATACTTTTACCCGGATAAAGGAGCAAAAAACAACGGCATTGACGGCGTGTATGGTCCGAAAACA
GCAGATGCAATTAGACGATTCCAGTCTATGTACGGGCTTACTCAAGACGGTATTTACGGACCAAAAACGAAAGCGAAACT
TGAAGCTCTCTTGAAGTAA

Upstream 100 bases:

>100_bases
CAATTGAGAACGAAAAAGAACAGAGTAAGAATAACGCAGACAAAGCTGCTGGCTAAGCCAGTGGCTTTTTTTATCTTACT
GACAGAAGGAGAGAGGGTAT

Downstream 100 bases:

>100_bases
ATAAATAGTCTCCTTGAGTATCTTTCTCAAGGAGATACTTATGTTTATTTTGTTACAGATAGTTCAAAATTATTCTAATA
TTTGAGTAAAAAGTATTATT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISYMKNNSSSTSFHFAVDDKQVIQGIPTNRNAWHTGDG
TNGTGNRKSIGVEICYSKSGGVRYKAAEKLAIKFVAQLLKERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAE
LKKLGGKTNSSKASVAKKKTTNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAALYFYPDKGAKNNGIDGVYGPKT
ADAIRRFQSMYGLTQDGIYGPKTKAKLEALLK

Sequences:

>Translated_272_residues
MAIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISYMKNNSSSTSFHFAVDDKQVIQGIPTNRNAWHTGDG
TNGTGNRKSIGVEICYSKSGGVRYKAAEKLAIKFVAQLLKERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAE
LKKLGGKTNSSKASVAKKKTTNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAALYFYPDKGAKNNGIDGVYGPKT
ADAIRRFQSMYGLTQDGIYGPKTKAKLEALLK
>Mature_271_residues
AIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISYMKNNSSSTSFHFAVDDKQVIQGIPTNRNAWHTGDGT
NGTGNRKSIGVEICYSKSGGVRYKAAEKLAIKFVAQLLKERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAEL
KKLGGKTNSSKASVAKKKTTNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAALYFYPDKGAKNNGIDGVYGPKTA
DAIRRFQSMYGLTQDGIYGPKTKAKLEALLK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLA_BACSU (P24808)

Other databases:

- EMBL:   X51424
- EMBL:   M59232
- EMBL:   D32216
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   S26671
- RefSeq:   NP_390467.1
- ProteinModelPortal:   P24808
- EnsemblBacteria:   EBBACT00000000013
- GeneID:   937772
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25900
- NMPDR:   fig|224308.1.peg.2592
- GenoList:   BSU25900
- GeneTree:   EBGT00050000000162
- HOGENOM:   HBG712727
- OMA:   IDENEVH
- ProtClustDB:   CLSK887114
- BioCyc:   BSUB:BSU25900-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002502
- InterPro:   IPR002477
- Gene3D:   G3DSA:3.40.80.10
- Gene3D:   G3DSA:1.10.101.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1; SSF55846 Amidase_2; SSF47090 PGBD_like

EC number: =3.5.1.28

Molecular weight: Translated: 29958; Mature: 29827

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISYMKNNSSSTSFHFAVD
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEC
DKQVIQGIPTNRNAWHTGDGTNGTGNRKSIGVEICYSKSGGVRYKAAEKLAIKFVAQLLK
HHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHH
ERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAELKKLGGKTNSSKASVAKKKT
HCCCCHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHC
TNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAALYFYPDKGAKNNGIDGVYGPKT
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
ADAIRRFQSMYGLTQDGIYGPKTKAKLEALLK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISYMKNNSSSTSFHFAVD
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEC
DKQVIQGIPTNRNAWHTGDGTNGTGNRKSIGVEICYSKSGGVRYKAAEKLAIKFVAQLLK
HHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHH
ERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAELKKLGGKTNSSKASVAKKKT
HCCCCHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHC
TNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAALYFYPDKGAKNNGIDGVYGPKT
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
ADAIRRFQSMYGLTQDGIYGPKTKAKLEALLK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2127796; 1683402; 7704261; 8969508; 9384377