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The map label for this gene is yqfS

Identifier: 16079568

GI number: 16079568

Start: 2593281

End: 2594174

Strand: Reverse

Name: yqfS

Synonym: BSU25130

Alternate gene names: 16079568

Gene position: 2594174-2593281 (Counterclockwise)

Preceding gene: 16079569

Following gene: 255767571

Centisome position: 61.54

GC content: 42.06

Gene sequence:

>894_bases
TTGCTGAGAATAGGCTCACACGTCTCTATGAGCGGAAAACATATGCTTCTGGCTGCAAGCCAGGAAGCAGTCTCTTATGG
GGCGAATACGTTTATGATCTATACAGGCGCACCGCAAAATACGCGCCGAAAGAAAATTGAAGACTTAAATATAGAAGCAG
GCCGGGCACATATGCAAGAAAACGGCATTGACGAGATTATCGTACACGCGCCTTATATTATTAATATTGGCAACACGACA
AATCCGTCCACTTTTGAGCTGGGTGTTGATTTTCTTCGTTCAGAAATTGAACGGACAGCGGCCATCGGAGCGAAGCAAAT
TGTCCTTCATCCTGGCGCTCACGTAGGGGCAGGAGCTGAAGCCGGCATCAAAAAAATTATTGAAGGCTTAAATGAAGTGA
TTGATCCGAATCAAAATGTCCAGATTGCATTAGAAACAATGGCTGGAAAAGGATCAGAATGCGGCAGAAGCTTTGAGGAG
CTTGCGCAGATTATCGAAGGCGTTACACACAATGAGCAGCTGTCAGTGTGCTTTGACACGTGCCATACACATGATGCCGG
GTATAACATTGTCGAAGACTTTGACGGAGTGCTAAATGAGTTCGATAAAATCATTGGCATTGACCGTATTAAAGTGCTTC
ATATCAATGACAGCAAAAATGTCAAAGGTGCGAGAAAAGACCGCCATGAAAATATTGGCTTTGGTGAAATAGGTTTTGAC
GCACTGCAATATGTTGTTCACCATGAGCAGTTAAAAGACATTCCAAAAATTCTTGAAACACCATACGTCGGTGAAGATAA
AAAGAACAAAAAACCGCCATACCGTTTTGAGATTGAAATGCTGAAAGAAAAACAATTTGATGATACATTGCTTGAAAAGA
TTTTACAGCAATAA

Upstream 100 bases:

>100_bases
GGTTAAGCCTGGGTATAAAAAGAAAATGAGCTATGAGATGGAGAAAATCAAGAAAAAACAGAGAAGAAACCAATCTAAGA
AAAGAAAGTAGGGGAACCTG

Downstream 100 bases:

>100_bases
AAAATGGGGGATAACACCCCCATTTTTTAGCTTGTAAATTGTTTGAATAGTTCATTAACTGTTTGAGCTGTTTCTTTCGA
TGTAATCGTCTCTACTTGTT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQENGIDEIIVHAPYIINIGNTT
NPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAEAGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEE
LAQIIEGVTHNEQLSVCFDTCHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFD
ALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ

Sequences:

>Translated_297_residues
MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQENGIDEIIVHAPYIINIGNTT
NPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAEAGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEE
LAQIIEGVTHNEQLSVCFDTCHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFD
ALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ
>Mature_297_residues
MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQENGIDEIIVHAPYIINIGNTT
NPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAEAGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEE
LAQIIEGVTHNEQLSVCFDTCHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFD
ALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=265, Percent_Identity=31.6981132075472, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=32.6996197718631, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322735, Length=265, Percent_Identity=30.9433962264151, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33069; Mature: 33069

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQE
CCEECCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
NGIDEIIVHAPYIINIGNTTNPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAE
CCCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
AGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEELAQIIEGVTHNEQLSVCFDT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
CHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFD
HHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCCCCHHHHH
ALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQE
CCEECCCCCCCCCEEEEEECHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
NGIDEIIVHAPYIINIGNTTNPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAE
CCCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHH
AGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEELAQIIEGVTHNEQLSVCFDT
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
CHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFD
HHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCCCCHHHHH
ALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA