Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is ispG

Identifier: 16079562

GI number: 16079562

Start: 2589123

End: 2590256

Strand: Direct

Name: ispG

Synonym: BSU25070

Alternate gene names: 16079562

Gene position: 2589123-2590256 (Clockwise)

Preceding gene: 16079561

Following gene: 16079564

Centisome position: 61.42

GC content: 46.38

Gene sequence:

>1134_bases
TTGCAAGTGAGTGAAATCACACATCGTACAAAAACGCGTCCCGTCAAAGTGGGACCTTTAACAATAGGCGGCAACAATGA
AGTTGTCATCCAAAGCATGACAACAACAAAAACACATGATGTAGAAGCAACGGTTGCGGAAATTAACCGTTTGGCTGAAG
CCGGATGCCAAATCGTTCGGGTAGCATGTCCGGATGAACGGGCGGCAAACGCCATTGCGGATATTAAAAAGCGCATTTCC
ATTCCTCTCGTTGTTGACATACATTTCGATTATAAACTTGCGTTGAAAGCCATTGAAGGCGGCGCAGATAAAATCCGAAT
CAACCCCGGCAATATCGGCCGGCGCGAAAAAGTTGAAGCGGTTGTTAAAGCGGCCAAAGACAAAGGCATTCCGATCAGAA
TCGGAGTAAACGCCGGTTCATTGGAAAAACGGATTTTAGAAAAATACGGTTATCCGACTGCCGATGGAATGGTAGAAAGC
GCACTTCATCACATTAAAATTCTTGAGGATCTTGATTTTCACGATATTATTGTCAGCATGAAGGCCTCTGACGTGAACCT
TGCAATCGAGGCTTATGAAAAAGCAGCGAAAGCGTTTGACTACCCGCTTCACCTCGGGATCACCGAGTCAGGAACACTGT
TTGCCGGCACAGTAAAGAGCGCAGCAGGACTCGGCGCCATTTTAAGCAAAGGCATCGGGAACACCATGCGCATTTCACTA
AGCGCAGACCCTGTAGAAGAGGTAAAAGTAGCAAGGGAGCTTCTGAAATCTTTCGGCTTAGCCTCCAATGCTGCCACGCT
CATCTCATGCCCGACTTGCGGCCGTATTGAGATTGATCTAATCAGCATTGCCAATGAAGTGGAAGAGTATATTTCTAAGA
TAAAAGCGCCGATTAAAGTTGCTGTTCTCGGCTGCGCTGTAAACGGACCTGGTGAAGCGAGAGAAGCTGATATCGGAATC
GCCGGCGCACGCGGTGAAGGGCTGCTGTTCCGGAAAGGGAAAATTGTCCGTAAGGTTCCAGAGGAAACGATGGTAGAAGA
ACTCAAAAAAGAAATTGACATTCTTGCTGAGGAACACTACGCAAAACTTGAAGCTGAAAAAGCAAAATTAAAAGAAGAAA
CACAAAAAGCTTGA

Upstream 100 bases:

>100_bases
TTCGGCGAAGACACTGTTACAATGTAATTTGTAAAATAAATTCACATAAACCATACTAGAATTGTTATATCTAGAAGCGG
CAACTACAATTAAGGAGCGA

Downstream 100 bases:

>100_bases
CGGGAACCCGTCAAGCTTTTTTGTGTTAGAAAAATGGAATAATAAATATTCCAAGCACTAATGAAACGACACCGATACCC
ATTGCCCAGGCTCCAAGGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAANAIADIKKRIS
IPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEAVVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVES
ALHHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISL
SADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKVAVLGCAVNGPGEAREADIGI
AGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHYAKLEAEKAKLKEETQKA

Sequences:

>Translated_377_residues
MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAANAIADIKKRIS
IPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEAVVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVES
ALHHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISL
SADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKVAVLGCAVNGPGEAREADIGI
AGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHYAKLEAEKAKLKEETQKA
>Mature_377_residues
MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAANAIADIKKRIS
IPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEAVVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVES
ALHHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISL
SADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKVAVLGCAVNGPGEAREADIGI
AGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHYAKLEAEKAKLKEETQKA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=345, Percent_Identity=48.9855072463768, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BACSU (P54482)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69955
- RefSeq:   NP_390386.1
- ProteinModelPortal:   P54482
- EnsemblBacteria:   EBBACT00000003088
- GeneID:   937984
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25070
- NMPDR:   fig|224308.1.peg.2511
- GenoList:   BSU25070
- GeneTree:   EBGT00050000002545
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   P54482
- ProtClustDB:   PRK00366
- BioCyc:   BSUB:BSU25070-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40584; Mature: 40584

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVR
CCCHHHHHHHCCCCEEECCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEE
VACPDERAANAIADIKKRISIPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEA
EECCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCEEEECCCCCCHHHHHHH
VVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSM
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEE
KASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISL
ECCCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEE
SADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCEEE
AVLGCAVNGPGEAREADIGIAGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHY
EEEEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHH
AKLEAEKAKLKEETQKA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVR
CCCHHHHHHHCCCCEEECCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEE
VACPDERAANAIADIKKRISIPLVVDIHFDYKLALKAIEGGADKIRINPGNIGRREKVEA
EECCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCEEEECCCCCCHHHHHHH
VVKAAKDKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSM
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEE
KASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTMRISL
ECCCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEE
SADPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISKIKAPIKV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCEEE
AVLGCAVNGPGEAREADIGIAGARGEGLLFRKGKIVRKVPEETMVEELKKEIDILAEEHY
EEEEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHH
AKLEAEKAKLKEETQKA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377