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The map label for this gene is folD

Identifier: 16079487

GI number: 16079487

Start: 2528404

End: 2529255

Strand: Reverse

Name: folD

Synonym: BSU24310

Alternate gene names: 16079487

Gene position: 2529255-2528404 (Counterclockwise)

Preceding gene: 255767541

Following gene: 255767540

Centisome position: 60.0

GC content: 45.54

Gene sequence:

>852_bases
ATGACTGCAACAATCATCGACGGAAAAGAAACGGCTAGAGAAAAACGCGAACAATTGGCAAAAGAGGTAGAAGAGCTTAA
AAAGCAAGGCGTCACTCCCGGACTGGCGGTTATTTTGATTGGAGATGATCCTGCTTCTCACTCTTACGTGCGAGGAAAGA
AAAAAGCGGCAGAAACAATGGGAATGAATTTCAAGCTCGATCAATTTGACAGCAGCCTCACAGAAGCTGAACTGCTTTCC
ATTATTGATCAATATAACCAAGACCCTGAGTTTCATGGCATTCTCGTTCAGCTTCCGCTCCCAGACCATATTTCTGAAAA
AGCGGTGATCGAACGTATCTCCCCTGATAAAGATGTAGACGGTTTTCATCCGTTAAACGTAGGGAAAATGCTGCTTGGCG
AAGATACGTTTCTTCCTTGCACGCCTCATGGAATTGTTGAGCTTTTGAAGAAAACCAATATTGACCTTTCCGGTAAAGAG
GTTGTTGTAGTCGGCCGAAGCAACATTGTCGGCAAACCTGTCGGCCAGCTTTTATTAAATGAAAACGCGACGGTTACTTA
CTGTCATTCAAGAACAGAAAATATTACTGAACATACGAAAAAAGCGGACATCCTGGTGGTGGCAGTCGGCAGAGCCAACT
TCATCAGTGCAGACCAGATCAAAGAGGGCGCTGTTGTCATTGACGTCGGCGTTAACCGTCTGGAAAACGGAAAGCTTTGC
GGAGACGTTGAATTTGAAGGGGCAAAGGAAAAAGCGTCCTTCATAACGCCGGTTCCGGGCGGAGTAGGTCCGATGACCAT
CACGATGCTTGCGCATAATACTGTTAAATCTGCGAAACGTACGTTATCATAG

Upstream 100 bases:

>100_bases
AGCTGGCAAAACGGTTTGGTGATGACAAAGCGACAAAGTTTGTAAACGGGGTTCTTTCTAACATTAAATCTGATATTGAA
CAATCATAGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
ATAATTGGAGATATTCACAGGCGAAATGTTTACCCGCCTTGAATATGAGCACGATACCCGCTTCTTCAGGCTCCTAAAGC
CAGGGCGGGTTTTCTTTAGT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTATIIDGKETAREKREQLAKEVEELKKQGVTPGLAVILIGDDPASHSYVRGKKKAAETMGMNFKLDQFDSSLTEAELLS
IIDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNIDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLC
GDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS

Sequences:

>Translated_283_residues
MTATIIDGKETAREKREQLAKEVEELKKQGVTPGLAVILIGDDPASHSYVRGKKKAAETMGMNFKLDQFDSSLTEAELLS
IIDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNIDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLC
GDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS
>Mature_282_residues
TATIIDGKETAREKREQLAKEVEELKKQGVTPGLAVILIGDDPASHSYVRGKKKAAETMGMNFKLDQFDSSLTEAELLSI
IDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNIDLSGKEV
VVVGRSNIVGKPVGQLLLNENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLCG
DVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=47.972972972973, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=45.2702702702703, Blast_Score=253, Evalue=9e-68,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=47.2602739726027, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI36796743, Length=272, Percent_Identity=25.7352941176471, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=51.9713261648746, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=42.1232876712329, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=44.6666666666667, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.7916666666667, Blast_Score=252, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6322933, Length=308, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=45.360824742268, Blast_Score=247, Evalue=6e-66,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=45.360824742268, Blast_Score=247, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=45.360824742268, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=45.360824742268, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=43.5810810810811, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=43.5810810810811, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30686; Mature: 30554

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIIDGKETAREKREQLAKEVEELKKQGVTPGLAVILIGDDPASHSYVRGKKKAAETM
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GMNFKLDQFDSSLTEAELLSIIDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVD
CCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNIDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLC
CCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCEE
GDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS
ECEECCCCCCCCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
TATIIDGKETAREKREQLAKEVEELKKQGVTPGLAVILIGDDPASHSYVRGKKKAAETM
CEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GMNFKLDQFDSSLTEAELLSIIDQYNQDPEFHGILVQLPLPDHISEKAVIERISPDKDVD
CCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNIDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLC
CCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCEE
GDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS
ECEECCCCCCCCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA