Click here to switch to the map view.

The map label for this gene is xseA

Identifier: 255767540

GI number: 255767540

Start: 2526920

End: 2528266

Strand: Reverse

Name: xseA

Synonym: BSU24300

Alternate gene names: 255767540

Gene position: 2528266-2526920 (Counterclockwise)

Preceding gene: 16079487

Following gene: 255767539

Centisome position: 59.97

GC content: 44.25

Gene sequence:

>1347_bases
ATGGGTGAAGCAGCGTATGTGACCGTCTCAGCGCTGACAAAGTATATAAAACGAAAATTCGATGTAGATCCTCACCTTGA
AAATATTTGGATTAAAGGCGAATTGTCGAATGTAAAGATACACACACGAGGGCATATTTATTTCACTTTAAAAGACGAAA
ACGCAAGAATGCAGTCGGTGATGTTCGCGAGACAAAGCGAGCGTCTGCCTTTTAAACCTGAAAACGGAATGAAAGTGCTG
GTCAGAGGCGGAATTTCCGTATATGAACCGAGCGGAAACTATCAATTATATGCCAAAGAAATGCAGCCTGACGGGGTTGG
AGCGCTTTATTTAGCGTACGAAGAATTAAAAAAGAAGCTTGCCGGAGAAGGTTTATTTGACGACCGCTACAAAAAACAAA
TCCCCGCATTTCCAGCCACAATCGGGGTTGTGACATCTCCAACGGGTGCCGCTGTCAGAGACGTTATTACAACTCTAAAA
AGAAGATATCCCCTTGTAAAAGTCATTGTTCTTCCAGCGCTCGTACAAGGTGAAAATGCGAGCAGATCCATCGTTACGCG
CATTGAAGAGGCAAATGAAAAAGAGATTTGCGACGTATTAATTGTCGGGAGAGGCGGCGGTTCGATAGAAGAACTGTGGG
CATTCAATGAAGAAATTGTAGCACGCGCCATCTTTGCTTCCAATATTCCGATTATATCGGCAGTCGGACATGAGACGGAT
TTTACGATTAGTGATTTTGTCGCCGATATCAGAGCTGCGACGCCGACAGGAGCAGCTGAGATTGCTGTGCCTCATACCAC
TGATTTAATCGAACGGACAAAAACAGCGGAAGTCAGAATGACAAGAGCAATGCAGCAGCATCTCGGCCAGAAAAAAGAAC
GGATACAAACGCTGCAATCGTCCTATGCCTTTCGTTTTCCAAAGCGTTTGTATGCGCAAAAAGAGCAGCAGTTTGACCTC
GCGTACCAGCAGTTTCAAGCACAGCTGACCGCTCTTTTAGATCGGAAAAGCAGACAGCTGGAGCGTGAAACGTACAGATT
AGAGGCATTGCATCCTCATGAACAGCTGAAACAAGCGAGAACACGATACCAGGAACAAACAAATCAGCTGAGAAAAAATA
TGAATATCCAAATGAAGCAGCTGCATTCCCAATTTCAAACCGTTCTTGGCAAGCTGAATGCACTAAGTCCTCTTCAAGTG
ATGGAAAGAGGATACAGCTTGGCTTATAAAGAAGATAAACTCATTAAAAGTGTCAGTCAAATAGAAGAGCAGGACCGGCT
TGAAATCAAGCTGAAGGATGGCGTGCTGACCTGTGAAGTATTAGAAAAGAGAGGGGAAGAAAAATGA

Upstream 100 bases:

>100_bases
CTTGAATATGAGCACGATACCCGCTTCTTCAGGCTCCTAAAGCCAGGGCGGGTTTTCTTTAGTTATGGTGCAGGAATGCT
TAGGAGAGGAGTTTTTAGAC

Downstream 100 bases:

>100_bases
CAGACGTGAAAAAAAATGAAAACATGACATTTGAAGAAGCGATGAAAGGGCTTGAGAGCATTGTATCAAAGCTTGAAGAG
GGAGATGTGCCTCTAGAGCA

Product: exodeoxyribonuclease VII large subunit

Products: NA

Alternate protein names: Exodeoxyribonuclease VII large subunit; Exonuclease VII large subunit

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MGEAAYVTVSALTKYIKRKFDVDPHLENIWIKGELSNVKIHTRGHIYFTLKDENARMQSVMFARQSERLPFKPENGMKVL
VRGGISVYEPSGNYQLYAKEMQPDGVGALYLAYEELKKKLAGEGLFDDRYKKQIPAFPATIGVVTSPTGAAVRDVITTLK
RRYPLVKVIVLPALVQGENASRSIVTRIEEANEKEICDVLIVGRGGGSIEELWAFNEEIVARAIFASNIPIISAVGHETD
FTISDFVADIRAATPTGAAEIAVPHTTDLIERTKTAEVRMTRAMQQHLGQKKERIQTLQSSYAFRFPKRLYAQKEQQFDL
AYQQFQAQLTALLDRKSRQLERETYRLEALHPHEQLKQARTRYQEQTNQLRKNMNIQMKQLHSQFQTVLGKLNALSPLQV
MERGYSLAYKEDKLIKSVSQIEEQDRLEIKLKDGVLTCEVLEKRGEEK

Sequences:

>Translated_448_residues
MGEAAYVTVSALTKYIKRKFDVDPHLENIWIKGELSNVKIHTRGHIYFTLKDENARMQSVMFARQSERLPFKPENGMKVL
VRGGISVYEPSGNYQLYAKEMQPDGVGALYLAYEELKKKLAGEGLFDDRYKKQIPAFPATIGVVTSPTGAAVRDVITTLK
RRYPLVKVIVLPALVQGENASRSIVTRIEEANEKEICDVLIVGRGGGSIEELWAFNEEIVARAIFASNIPIISAVGHETD
FTISDFVADIRAATPTGAAEIAVPHTTDLIERTKTAEVRMTRAMQQHLGQKKERIQTLQSSYAFRFPKRLYAQKEQQFDL
AYQQFQAQLTALLDRKSRQLERETYRLEALHPHEQLKQARTRYQEQTNQLRKNMNIQMKQLHSQFQTVLGKLNALSPLQV
MERGYSLAYKEDKLIKSVSQIEEQDRLEIKLKDGVLTCEVLEKRGEEK
>Mature_447_residues
GEAAYVTVSALTKYIKRKFDVDPHLENIWIKGELSNVKIHTRGHIYFTLKDENARMQSVMFARQSERLPFKPENGMKVLV
RGGISVYEPSGNYQLYAKEMQPDGVGALYLAYEELKKKLAGEGLFDDRYKKQIPAFPATIGVVTSPTGAAVRDVITTLKR
RYPLVKVIVLPALVQGENASRSIVTRIEEANEKEICDVLIVGRGGGSIEELWAFNEEIVARAIFASNIPIISAVGHETDF
TISDFVADIRAATPTGAAEIAVPHTTDLIERTKTAEVRMTRAMQQHLGQKKERIQTLQSSYAFRFPKRLYAQKEQQFDLA
YQQFQAQLTALLDRKSRQLERETYRLEALHPHEQLKQARTRYQEQTNQLRKNMNIQMKQLHSQFQTVLGKLNALSPLQVM
ERGYSLAYKEDKLIKSVSQIEEQDRLEIKLKDGVLTCEVLEKRGEEK

Specific function: Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides

COG id: COG1570

COG function: function code L; Exonuclease VII, large subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xseA family

Homologues:

Organism=Escherichia coli, GI1788856, Length=437, Percent_Identity=40.7322654462243, Blast_Score=334, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EX7L_BACSU (P54521)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   G69960
- RefSeq:   NP_390310.2
- EnsemblBacteria:   EBBACT00000001644
- GeneID:   938650
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24300
- NMPDR:   fig|224308.1.peg.2434
- GenoList:   BSU24300
- GeneTree:   EBGT00050000001906
- HOGENOM:   HBG634411
- PhylomeDB:   P54521
- ProtClustDB:   PRK00286
- BioCyc:   BSUB:BSU24300-MONOMER
- BRENDA:   3.1.11.6
- GO:   GO:0005737
- HAMAP:   MF_00378
- InterPro:   IPR003753
- InterPro:   IPR020579
- InterPro:   IPR004365
- TIGRFAMs:   TIGR00237

Pfam domain/function: PF02601 Exonuc_VII_L; PF01336 tRNA_anti

EC number: =3.1.11.6

Molecular weight: Translated: 51048; Mature: 50917

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAAYVTVSALTKYIKRKFDVDPHLENIWIKGELSNVKIHTRGHIYFTLKDENARMQSV
CCCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECEEEEEECCEEEEEEECCCCHHHHH
MFARQSERLPFKPENGMKVLVRGGISVYEPSGNYQLYAKEMQPDGVGALYLAYEELKKKL
HHHHHCCCCCCCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
AGEGLFDDRYKKQIPAFPATIGVVTSPTGAAVRDVITTLKRRYPLVKVIVLPALVQGENA
CCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
SRSIVTRIEEANEKEICDVLIVGRGGGSIEELWAFNEEIVARAIFASNIPIISAVGHETD
CHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCC
FTISDFVADIRAATPTGAAEIAVPHTTDLIERTKTAEVRMTRAMQQHLGQKKERIQTLQS
CCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SYAFRFPKRLYAQKEQQFDLAYQQFQAQLTALLDRKSRQLERETYRLEALHPHEQLKQAR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TRYQEQTNQLRKNMNIQMKQLHSQFQTVLGKLNALSPLQVMERGYSLAYKEDKLIKSVSQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEHHHHHHHHHHH
IEEQDRLEIKLKDGVLTCEVLEKRGEEK
HHHCCCEEEEECCCEEEHHHHHHCCCCC
>Mature Secondary Structure 
GEAAYVTVSALTKYIKRKFDVDPHLENIWIKGELSNVKIHTRGHIYFTLKDENARMQSV
CCCCEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECEEEEEECCEEEEEEECCCCHHHHH
MFARQSERLPFKPENGMKVLVRGGISVYEPSGNYQLYAKEMQPDGVGALYLAYEELKKKL
HHHHHCCCCCCCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
AGEGLFDDRYKKQIPAFPATIGVVTSPTGAAVRDVITTLKRRYPLVKVIVLPALVQGENA
CCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
SRSIVTRIEEANEKEICDVLIVGRGGGSIEELWAFNEEIVARAIFASNIPIISAVGHETD
CHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCC
FTISDFVADIRAATPTGAAEIAVPHTTDLIERTKTAEVRMTRAMQQHLGQKKERIQTLQS
CCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SYAFRFPKRLYAQKEQQFDLAYQQFQAQLTALLDRKSRQLERETYRLEALHPHEQLKQAR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TRYQEQTNQLRKNMNIQMKQLHSQFQTVLGKLNALSPLQVMERGYSLAYKEDKLIKSVSQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEHHHHHHHHHHH
IEEQDRLEIKLKDGVLTCEVLEKRGEEK
HHHCCCEEEEECCCEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377