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The map label for this gene is mmgD

Identifier: 16079470

GI number: 16079470

Start: 2509654

End: 2510772

Strand: Reverse

Name: mmgD

Synonym: BSU24140

Alternate gene names: 16079470

Gene position: 2510772-2509654 (Counterclockwise)

Preceding gene: 255767530

Following gene: 255767529

Centisome position: 59.56

GC content: 47.36

Gene sequence:

>1119_bases
ATGGAGGAGAAACAGCATTATTCTCCAGGGTTGGACGGTGTGATTGCGGCAGAAACTCATATTTCTTATCTTGACACGCA
GTCCAGCCAGATTTTGATAAGAGGCTATGATTTAATAGAACTTTCTGAAACAAAAAGCTATCTGGAGCTTGTCCATTTAT
TGCTGGAGGGCAGGCTGCCAGAAGAAAGTGAAATGGAAACGCTGGAACGTAAAATAAACAGTGCCTCAAGCCTGCCTGCT
GATCATCTCCGTCTGCTTGAGCTGCTCCCTGAGGACACTCATCCAATGGATGGTCTGCGTACCGGCCTGTCGGCACTTGC
TGGCTATGATCGGCAAATTGATGATCGGTCTCCTTCCGCAAACAAAGAACGCGCCTATCAGCTTCTTGGGAAAATGCCTG
CCCTCACTGCTGCCAGCTACAGGATCATTAATAAGAAAGAACCGATTCTTCCGCTTCAAACCCTATCCTACAGTGCGAAT
TTTCTCTACATGATGACAGGGAAATTGCCGTCTTCATTGGAAGAACAGATTTTTGACCGGTCGCTCGTCCTGTACAGTGA
GCATGAGATGCCGAATTCAACCTTTGCGGCCAGGGTCATTGCATCTACACATTCAGATCTTTATGGGGCTTTGACTGGGG
CTGTCGCTTCCCTGAAAGGGAATCTTCACGGAGGAGCAAACGAAGCTGTGATGTATTTGCTTTTGGAGGCAAAAACGACA
TCTGATTTTGAGCAGCTTTTGCAAACAAAACTGAAAAGAAAAGAAAAAATCATGGGCTTCGGCCACCGGGTGTATATGAA
AAAAATGGACCCGAGAGCACTCATGATGAAAGAGGCGCTGCAGCAATTATGCGATAAAGCCGGTGACCATCGTTTATATG
AGATGTGCGAAGCGGGTGAGCGGCTGATGGAGAAGGAAAAAGGGCTTTACCCGAATTTGGATTACTATGCTGCCCCAGTG
TATTGGATGCTCGGCATACCGATTCCTTTATATACGCCGATCTTTTTCAGCGCGAGAACGTCCGGTTTATGCGCCCATGT
GATCGAACAGCATGCCAATAATCGGCTCTTCCGTCCCCGTGTCAGTTACATGGGCCCGCGATATCAAACAAAATCATGA

Upstream 100 bases:

>100_bases
AATTTATGAAGGGACGAACGAGATTCAAAGACTGATTATTTCCAAGTATTTGCTTGGCGGAACCTGATAGACAAAAAACG
CAAAAACGGAGGTTGAGGAT

Downstream 100 bases:

>100_bases
AAGGAGCTGGTAGAATGCCGAAAACGGATCGTGTTATCGAAGAAATCACAGACTATGTGCTTGAGAAAGAGATTACTAGT
GCCGAAGCGTATACAACAGC

Product: citrate synthase 3

Products: NA

Alternate protein names: Citrate synthase 3; Citrate synthase III; Methylcitrate synthase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLPEESEMETLERKINSASSLPA
DHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSANKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSAN
FLYMMTGKLPSSLEEQIFDRSLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTT
SDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGERLMEKEKGLYPNLDYYAAPV
YWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPRVSYMGPRYQTKS

Sequences:

>Translated_372_residues
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLPEESEMETLERKINSASSLPA
DHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSANKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSAN
FLYMMTGKLPSSLEEQIFDRSLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTT
SDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGERLMEKEKGLYPNLDYYAAPV
YWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPRVSYMGPRYQTKS
>Mature_372_residues
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLPEESEMETLERKINSASSLPA
DHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSANKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSAN
FLYMMTGKLPSSLEEQIFDRSLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTT
SDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGERLMEKEKGLYPNLDYYAAPV
YWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPRVSYMGPRYQTKS

Specific function: Catalyzes the synthesis of citrate from acetyl-CoA and oxaloacetate. The genomic context suggests that this protein probably functions as a 2-methylcitrate synthase, i.e. catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=326, Percent_Identity=25.1533742331288, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786527, Length=360, Percent_Identity=35.8333333333333, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1786939, Length=374, Percent_Identity=31.0160427807487, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17555174, Length=367, Percent_Identity=26.9754768392371, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=349, Percent_Identity=28.080229226361, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=350, Percent_Identity=24.5714285714286, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640124, Length=377, Percent_Identity=25.9946949602122, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640126, Length=377, Percent_Identity=25.4641909814324, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356863, Length=331, Percent_Identity=22.6586102719033, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CISY3_BACSU (P45858)

Other databases:

- EMBL:   U29084
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   E69658
- RefSeq:   NP_390294.1
- ProteinModelPortal:   P45858
- SMR:   P45858
- EnsemblBacteria:   EBBACT00000002129
- GeneID:   938665
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24140
- NMPDR:   fig|224308.1.peg.2418
- GenoList:   BSU24140
- GeneTree:   EBGT00050000001635
- HOGENOM:   HBG649489
- OMA:   SANKERA
- PhylomeDB:   P45858
- ProtClustDB:   PRK12349
- BioCyc:   BSUB:BSU24140-MONOMER
- BRENDA:   2.3.3.1
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.5; =2.3.3.1

Molecular weight: Translated: 42107; Mature: 42107

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 262-262 ACT_SITE 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLP
CCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHCCCC
EESEMETLERKINSASSLPADHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSANFLYMMTGKLPSSLEEQIFDR
CHHHHHHHHHCCCHHHHHHHHEECCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHCC
SLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
SDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGE
HHHHHHHHHHHHHHHHHHCCCHHEEHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RLMEKEKGLYPNLDYYAAPVYWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPR
HHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCH
VSYMGPRYQTKS
HHHCCCCCCCCC
>Mature Secondary Structure
MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLP
CCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHCCCC
EESEMETLERKINSASSLPADHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
NKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSANFLYMMTGKLPSSLEEQIFDR
CHHHHHHHHHCCCHHHHHHHHEECCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHCC
SLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
SDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGE
HHHHHHHHHHHHHHHHHHCCCHHEEHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RLMEKEKGLYPNLDYYAAPVYWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPR
HHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCH
VSYMGPRYQTKS
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377