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The map label for this gene is prpD

Identifier: 255767529

GI number: 255767529

Start: 2508221

End: 2509639

Strand: Reverse

Name: prpD

Synonym: BSU24130

Alternate gene names: 255767529

Gene position: 2509639-2508221 (Counterclockwise)

Preceding gene: 16079470

Following gene: 16079468

Centisome position: 59.53

GC content: 47.43

Gene sequence:

>1419_bases
ATGCCGAAAACGGATCGTGTTATCGAAGAAATCACAGACTATGTGCTTGAGAAAGAGATTACTAGTGCCGAAGCGTATAC
AACAGCGGGGCATGTATTGCTGGATACCCTGGGCTGCGGAATCTTGGCGCTACGGTATCCGGAATGCACAAAGCTGCTTG
GCCCGATTGTTCCAGGAACGACTGTGCCGAATGGAAGCAAAGTGCCGGGAACGTCATATGTTCTCGATCCTGTGAGGGCA
GCGTTTAATATTGGCTGCATGATTCGCTGGCTCGATTACAACGATACGTGGCTGGCGGCGGAGTGGGGGCATCCATCCGA
TAACTTGGGAGGAATCCTTGCCGCTGCTGATTATGTATCAAGAGTGAGGCTGTCTGAAGGAAAAGAGCCGTTAACGGTAC
GCGACGTACTTGAGATGATGATCAAAGCACACGAGATCCAAGGTGTGCTGGCGTTAGAAAACAGCTTAAATCGAGTTGGC
CTTGACCATGTGCTGTTTGTCAAAGTTGCGACAACTGCTGTTGCAGCAAAGCTTTTGGGCGGCGGAAGAGAAGAAATCAA
AAATGCGCTTTCGAATGCATGGATTGACAATGCAGCATTGAGGACATACCGCCATTCACCGAATACAGGCTCGCGTAAAT
CATGGGCGGCAGGAGATGCAACGAGCAGAGGTGTTCATTTGGCGCTGATGTCGTTAAAAGGGGAAATGGGTTATCCGACA
GCCTTGAGTGCACCGGGTTGGGGGTTCCAAGACGTTTTATTCAATAAGAAAGAGATCAAGCTTGCCCGTCCGCTCGACGC
TTATGTCATGGAAAATGTGTTATTTAAAGTGTCTTATCCTGCGGAATTTCACGCCCAGACAGCGGCGGAGTCCGCCGTCA
TTCTTCACCCGCAGGTAAAGAACCGGATTGACGAGATTGACCGAGTTGTCATCAGAACGCATGAATCAGCTATCCGAATC
ATTGATAAAAAAGGCCCGCTCCACAATCCGGCAGACCGTGATCACTGCCTCCAATACATTACAGCAATCGGTTTGCTTTT
TGGTGATATCACCGCACAGCATTATGAAGCTGAAACAGCGAATGATCCGAGAATAGATAAACTTCGTGATAAAATGGAAG
TGACTGAAAATAAAACATATACCGAGGATTATTTGAAGCCTGATAAACGCTCCATCTCAAATGCCGTTCAGGTTCATTTT
AAAGATGGAACGAGCACGGAAATGGTGGAATGTGAGTTTCCCCTCGGCCATCGTTTTCGAAGAGAGGAAGCAGTGCCGAA
ACTGCTGGAAAAGTTTTCTGACAATCTCAAAACCCATTTTCCTGATAAACAGCACAAACACATTTATGAGCGCTGTACCA
GCTATGAGACATTACAAACGATGCGCGTAAATGAATTCGTAGACATGTTCTGCATGTAA

Upstream 100 bases:

>100_bases
CCCATGTGATCGAACAGCATGCCAATAATCGGCTCTTCCGTCCCCGTGTCAGTTACATGGGCCCGCGATATCAAACAAAA
TCATGAAAGGAGCTGGTAGA

Downstream 100 bases:

>100_bases
AAGGGGGATTTTGTTTCATGTCGTGGATCGTCAATAAGCAATCGTCACAAGAGGAGCTTGCCGGGCGTTTCCGCAAGCTG
ATGTCAGCACCGGACATTCT

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGTTVPNGSKVPGTSYVLDPVRA
AFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVSRVRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVG
LDHVLFVKVATTAVAAKLLGGGREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPT
ALSAPGWGFQDVLFNKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAAESAVILHPQVKNRIDEIDRVVIRTHESAIRI
IDKKGPLHNPADRDHCLQYITAIGLLFGDITAQHYEAETANDPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHF
KDGTSTEMVECEFPLGHRFRREEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVDMFCM

Sequences:

>Translated_472_residues
MPKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGTTVPNGSKVPGTSYVLDPVRA
AFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVSRVRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVG
LDHVLFVKVATTAVAAKLLGGGREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPT
ALSAPGWGFQDVLFNKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAAESAVILHPQVKNRIDEIDRVVIRTHESAIRI
IDKKGPLHNPADRDHCLQYITAIGLLFGDITAQHYEAETANDPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHF
KDGTSTEMVECEFPLGHRFRREEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVDMFCM
>Mature_471_residues
PKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGTTVPNGSKVPGTSYVLDPVRAA
FNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVSRVRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVGL
DHVLFVKVATTAVAAKLLGGGREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPTA
LSAPGWGFQDVLFNKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAAESAVILHPQVKNRIDEIDRVVIRTHESAIRII
DKKGPLHNPADRDHCLQYITAIGLLFGDITAQHYEAETANDPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHFK
DGTSTEMVECEFPLGHRFRREEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVDMFCM

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family

Homologues:

Organism=Escherichia coli, GI1786528, Length=474, Percent_Identity=60.9704641350211, Blast_Score=613, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6325258, Length=482, Percent_Identity=52.6970954356847, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PRPD_BACSU (P45859)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   U29084
- PIR:   F69658
- RefSeq:   NP_390293.2
- ProteinModelPortal:   P45859
- SMR:   P45859
- EnsemblBacteria:   EBBACT00000000463
- GeneID:   938663
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24130
- NMPDR:   fig|224308.1.peg.2417
- GenoList:   BSU24130
- GeneTree:   EBGT00050000002507
- HOGENOM:   HBG298383
- PhylomeDB:   P45859
- ProtClustDB:   PRK09425
- BioCyc:   BSUB:BSU24130-MONOMER
- InterPro:   IPR012705
- InterPro:   IPR005656
- PANTHER:   PTHR16943
- TIGRFAMs:   TIGR02330

Pfam domain/function: PF03972 MmgE_PrpD; SSF103378 MmgE_PrpD

EC number: =4.2.1.79

Molecular weight: Translated: 52879; Mature: 52748

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCC
TVPNGSKVPGTSYVLDPVRAAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVS
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
RVRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVGLDHVLFVKVATTAVAAKLLG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHC
GGREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPT
CCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
ALSAPGWGFQDVLFNKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAAESAVILHPQVK
CCCCCCCCHHHHHCCHHHHEECCCHHHHHHHHHHEEECCCHHHHHHHHCCCEEEECHHHH
NRIDEIDRVVIRTHESAIRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITAQHYEAETA
HHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NDPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHFKDGTSTEMVECEFPLGHRFR
CCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCEEEEEECCCCCCCEEEECCCCCHHHH
REEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVDMFCM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCC
TVPNGSKVPGTSYVLDPVRAAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVS
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
RVRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVGLDHVLFVKVATTAVAAKLLG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHC
GGREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPT
CCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
ALSAPGWGFQDVLFNKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAAESAVILHPQVK
CCCCCCCCHHHHHCCHHHHEECCCHHHHHHHHHHEEECCCHHHHHHHHCCCEEEECHHHH
NRIDEIDRVVIRTHESAIRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITAQHYEAETA
HHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NDPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHFKDGTSTEMVECEFPLGHRFR
CCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHCCEEEEEECCCCCCCEEEECCCCCHHHH
REEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVDMFCM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838