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The map label for this gene is prpB

Identifier: 16079468

GI number: 16079468

Start: 2507298

End: 2508203

Strand: Reverse

Name: prpB

Synonym: BSU24120

Alternate gene names: 16079468

Gene position: 2508203-2507298 (Counterclockwise)

Preceding gene: 255767529

Following gene: 16079466

Centisome position: 59.5

GC content: 49.78

Gene sequence:

>906_bases
ATGTCGTGGATCGTCAATAAGCAATCGTCACAAGAGGAGCTTGCCGGGCGTTTCCGCAAGCTGATGTCAGCACCGGACAT
TCTTCAAATTCCCGGCGCCCATGACGGGATGGCCGCTTTGCTTGCGAAAGAAGCCGGTTTTTCTGCTATTTATTTGTCCG
GTGCTGCCTATACAGCCAGCAGGGGGCTGCCTGATTTAGGGATCATCACATCGGCAGAGATAGCTGAACGGGCCAAAGAT
CTTGTGCGTGCAGCTGATCTGCCTTTGCTTGTGGATATCGATACGGGATTTGGCGGTGTCTTAAATGCCGCCCGAACAGC
CCGGGAGATGCTTGAAGCCAGGGTAGCAGCCGTTCAAATGGAAGACCAGCAGCTCCCAAAAAAATGCGGACATTTAAATG
GCAAACAGCTAGTTCCGATAAAGGAAATGGCGCAAAAAATAAAAGCCATTAAACAAGCGGCACCCTCCCTTATTGTTGTT
GCCCGTACTGATGCCAGGGCGCAGGAAGGGCTTGATGCCGCAATAAAACGTTCAGAGGCTTACATTGAGGCGGGAGCGGA
CGCAATTTTCCCGGAAGCGCTACAGGCGGAGAATGAATTCCGCCAGTTTGCTGAGCGTATCCCCGTTCCGCTCCTTGCGA
ATATGACTGAATTTGGGAAAACGCCATATTATCGTGCCGATGAATTCGAAGATATGGGGTTTCACATGGTGATATATCCT
GTCACCTCGCTTCGAGCGGCGGCAAAAGCTTGTGAGCGGATGTTTGGTTTGATGAAGGAGCACGGCTCACAAAAAGAAGG
ACTTCATGATATGCAGACACGAAAAGAACTGTACGATACGATTTCTTATTATGACTATGAAGCGTTAGACAAAACGATTG
CCAAGACCGTTTTGCCTGACGAATAA

Upstream 100 bases:

>100_bases
AACACATTTATGAGCGCTGTACCAGCTATGAGACATTACAAACGATGCGCGTAAATGAATTCGTAGACATGTTCTGCATG
TAAAAGGGGGATTTTGTTTC

Downstream 100 bases:

>100_bases
TGCAACAAAAAAAGCCGGAGCATTCTCCGGCTTTTTTTCAGCTATCGTTTTCTGAATCGCGGGCTGACTTGATTGCGTTT
TCTGTCTCTGTATAAAATGA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGAAYTASRGLPDLGIITSAEIAERAKD
LVRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQMEDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAAPSLIVV
ARTDARAQEGLDAAIKRSEAYIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRADEFEDMGFHMVIYP
VTSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPDE

Sequences:

>Translated_301_residues
MSWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGAAYTASRGLPDLGIITSAEIAERAKD
LVRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQMEDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAAPSLIVV
ARTDARAQEGLDAAIKRSEAYIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRADEFEDMGFHMVIYP
VTSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPDE
>Mature_300_residues
SWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGAAYTASRGLPDLGIITSAEIAERAKDL
VRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQMEDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAAPSLIVVA
RTDARAQEGLDAAIKRSEAYIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRADEFEDMGFHMVIYPV
TSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPDE

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=284, Percent_Identity=44.3661971830986, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1790445, Length=256, Percent_Identity=31.640625, Blast_Score=87, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71982926, Length=204, Percent_Identity=32.3529411764706, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=204, Percent_Identity=32.3529411764706, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320908, Length=104, Percent_Identity=33.6538461538462, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPB_BACSU (P54528)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   G69961
- RefSeq:   NP_390292.1
- ProteinModelPortal:   P54528
- SMR:   P54528
- EnsemblBacteria:   EBBACT00000002241
- GeneID:   938668
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24120
- NMPDR:   fig|224308.1.peg.2416
- GenoList:   BSU24120
- GeneTree:   EBGT00050000001597
- HOGENOM:   HBG728656
- OMA:   GMTSLND
- PhylomeDB:   P54528
- ProtClustDB:   CLSK873755
- BioCyc:   BSUB:BSU24120-MONOMER
- BRENDA:   2.7.8.23
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR02317

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.30

Molecular weight: Translated: 33097; Mature: 32966

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGAAYTAS
CCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHH
RGLPDLGIITSAEIAERAKDLVRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQM
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAAPSLIVVARTDARAQEGLDAAIKRSEA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
YIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRADEFEDMGFHMVIYP
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCCCCCCCHHCCCCEEEEEH
VTSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
E
C
>Mature Secondary Structure 
SWIVNKQSSQEELAGRFRKLMSAPDILQIPGAHDGMAALLAKEAGFSAIYLSGAAYTAS
CCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHH
RGLPDLGIITSAEIAERAKDLVRAADLPLLVDIDTGFGGVLNAARTAREMLEARVAAVQM
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EDQQLPKKCGHLNGKQLVPIKEMAQKIKAIKQAAPSLIVVARTDARAQEGLDAAIKRSEA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
YIEAGADAIFPEALQAENEFRQFAERIPVPLLANMTEFGKTPYYRADEFEDMGFHMVIYP
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCCCCCCCHHCCCCEEEEEH
VTSLRAAAKACERMFGLMKEHGSQKEGLHDMQTRKELYDTISYYDYEALDKTIAKTVLPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377